Strain identifier

BacDive ID: 8393

Type strain: Yes

Species: Mycobacterium kansasii

Strain Designation: G133 Bostrom, 14 011 0001

Strain history: CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 011 0001 <- 1968, ATCC <- A. Pollack: strain G133 Bostrom

NCBI tax ID(s): 557599 (strain), 1768 (species)

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General

@ref: 11560

BacDive-ID: 8393

DSM-Number: 44162

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, human pathogen

description: Mycobacterium kansasii G133 Bostrom is an obligate aerobe, mesophilic human pathogen that was isolated from fatal case.

NCBI tax id

NCBI tax idMatching level
557599strain
1768species

strain history

@refhistory
11560<- ATCC; ATCC 12478 <- A. Pollak; G133 Bostrom
67770M. Tsukamura 07002 <-- T. Takeya <-- E. H. Runyon P-16.
120378CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 011 0001 <- 1968, ATCC <- A. Pollack: strain G133 Bostrom

doi: 10.13145/bacdive8393.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium kansasii
  • full scientific name: Mycobacterium kansasii Hauduroy 1955 (Approved Lists 1980)

@ref: 11560

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium kansasii

full scientific name: Mycobacterium kansasii Hauduroy 1955 emend. Nouioui et al. 2018

strain designation: G133 Bostrom, 14 011 0001

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no90.643
69480100positive
120378norod-shaped

multimedia

@refmultimedia contentintellectual property rightscaption
11560https://www.dsmz.de/microorganisms/photos/DSM_44162-1.jpg© Leibniz-Institut DSMZ
11560https://www.dsmz.de/microorganisms/photos/DSM_44162.jpg© Leibniz-Institut DSMZMedium 645 37°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11560PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250)yeshttps://mediadive.dsmz.de/medium/250Name: PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250) Composition: Agar 20.0 g/l Proteose peptone no. 3 5.0 g/l Meat extract 3.0 g/l Glycerol Distilled water
11560MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
36241MEDIUM 55 - for Mycobacteriumyes
120378CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
11560positivegrowth37mesophilic
36241positivegrowth37mesophilic
67770positivegrowth37mesophilic
120378positivegrowth25-41
120378nogrowth10psychrophilic
120378nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120378
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.799

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
120378606565hippurate-hydrolysis
12037817632nitrate+reduction
12037816301nitrite-reduction
12037817632nitrate-respiration

metabolite production

  • @ref: 120378
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12037815688acetoin-
12037817234glucose+

enzymes

@refvalueactivityec
120378oxidase-
120378beta-galactosidase-3.2.1.23
120378alcohol dehydrogenase-1.1.1.1
120378gelatinase-
120378amylase-
120378DNase-
120378caseinase-3.4.21.50
120378catalase+1.11.1.6
120378tween esterase-
120378gamma-glutamyltransferase-2.3.2.2
120378lecithinase-
120378lipase-
120378lysine decarboxylase-4.1.1.18
120378ornithine decarboxylase-4.1.1.17
120378protease-
120378tryptophan deaminase-
120378urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120378-+++-+-+--++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11560fatal case
67770Human inguinal sinusKansas City, MOUSAUSANorth America
120378Fatal case

taxonmaps

  • @ref: 69479
  • File name: preview.99_75.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_37;97_39;98_43;99_75&stattab=map
  • Last taxonomy: Mycobacterium
  • 16S sequence: AJ536035
  • Sequence Identity:
  • Total samples: 1520
  • soil counts: 881
  • aquatic counts: 382
  • animal counts: 203
  • plant counts: 54

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
11560yesyes2Risk group (German classification)
1203782Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium kansasii strain ATCC12478 16S ribosomal RNA (rrs) gene, partial sequenceAF059848113ena557599
20218Mycobacterium kansasii 16S ribosomal RNA gene, partial sequenceAF4806011469ena557599
20218Mycobacterium kansasii 16S ribosomal RNA (16S rRNA) gene and 23S ribosomal RNA (23S rRNA) geneL42262432ena1768
20218M.kansasii 16S ribosomal RNAM295751369ena1768
20218Mycobacterium kansasii strain CIP 104589 16S ribosomal RNA gene, partial sequenceAF547940554ena1768
20218Mycobacterium kansasii DNA for 16S rRNA, internal transcribed spacer, 23S rRNAAB026695432ena1768
11560Mycobacterium kansasii 16S rRNA geneAJ5360351470ena1768

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium kansasii ATCC 12478GCA_000157895chromosomencbi557599
66792Mycobacterium kansasii ATCC 12478557599.3wgspatric557599
66792Mycobacterium kansasii ATCC 12478557599.62completepatric557599
66792Mycobacterium kansasii ATCC 12478557599.57plasmidpatric557599
66792Mycobacterium kansasii ATCC 124782563366550completeimg557599

GC content

  • @ref: 67770
  • GC-content: 66.2
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno81no
motileno95.271no
flagellatedno98.891no
gram-positiveyes87.015no
anaerobicno98.552no
aerobicyes88.473no
halophileno93.533no
spore-formingno86.064no
thermophileno98.457yes
glucose-utilyes63.78no
glucose-fermentno91.155no

External links

@ref: 11560

culture collection no.: DSM 44162, ATCC 12478, JCM 6379, TMC 1204, CCUG 20997, CCUG 27787, CECT 3030, CIP 104589, KCTC 9515, NCTC 13024, RIMD 1320001

straininfo link

  • @ref: 77792
  • straininfo: 44426

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7629856Demonstration of Mycobacterium kansasii species heterogeneity by the amplification of the 16S-23S spacer region.Abed Y, Bollet C, De Micco PJ Med Microbiol10.1099/00222615-43-2-1561995Base Sequence, DNA Primers/chemistry, DNA, Ribosomal/*chemistry, Genetic Variation, Humans, Molecular Sequence Data, Nontuberculous Mycobacteria/classification/*genetics, Polymerase Chain Reaction, RNA, Ribosomal, 16S/*genetics, RNA, Ribosomal, 23S/*genetics, RNA, Ribosomal, 5S/geneticsPathogenicity
Phylogeny9041396Large-restriction-fragment analysis of Mycobacterium kansasii genomic DNA and its application in molecular typing.Iinuma Y, Ichiyama S, Hasegawa Y, Shimokata K, Kawahara S, Matsushima TJ Clin Microbiol10.1128/jcm.35.3.596-599.19971997*Bacterial Typing Techniques/statistics & numerical data, Base Sequence, DNA Primers/genetics, Evaluation Studies as Topic, Humans, Japan/epidemiology, Mycobacterium Infections, Nontuberculous/epidemiology/microbiology, Nontuberculous Mycobacteria/*classification/*genetics/isolation & purification, Occupational Diseases/epidemiology/microbiology, *Polymorphism, Restriction Fragment Length, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Reproducibility of ResultsPathogenicity
Cultivation14744453Characterization of the susceptibility of mycobacteria in BACTEC 12B media containing PANTA that had been supplemented with ceftazidime, and characterization of the individual components of PANTA in the presence of C18-carboxypropylbetaine.Thornton CG, MacLellan KM, Brink TL, Passen SJ Microbiol Methods10.1016/j.mimet.2003.10.0202004Anti-Bacterial Agents/*pharmacology, Azlocillin/pharmacology, Bacteriological Techniques/*methods, Betaine/*analogs & derivatives/*pharmacology, Ceftazidime/*pharmacology, Culture Media, Mycobacterium/*drug effects/growth & development, Nalidixic Acid/pharmacology, Polymyxin B/pharmacology, Trimethoprim/pharmacologyPathogenicity
Enzymology15722145Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean.Leao SC, Bernardelli A, Cataldi A, Zumarraga M, Robledo J, Realpe T, Mejia GI, da Silva Telles MA, Chimara E, Velazco M, Fernandez J, Rodrigues PA, Guerrero MI, Leon CI, Porras TB, Rastogi N, Goh KS, Suffys P, da Silva Rocha A, dos Santos Netto D, Ritacco V, Lopez B, Barrera L, Palomino JC, Martin A, Portaels FJ Microbiol Methods10.1016/j.mimet.2004.11.0152004Bacterial Proteins/chemistry/genetics, Chaperonin 60, Chaperonins/chemistry/genetics, DNA, Bacterial/chemistry/genetics, Guadeloupe, Humans, Mycobacterium/genetics/*isolation & purification, Mycobacterium Infections/*diagnosis, Polymerase Chain Reaction/*methods/standards, Restriction Mapping, South AmericaPathogenicity
Phylogeny20833878Mycobacterium shinjukuense sp. nov., a slowly growing, non-chromogenic species isolated from human clinical specimens.Saito H, Iwamoto T, Ohkusu K, Otsuka Y, Akiyama Y, Sato S, Taguchi O, Sueyasu Y, Kawabe Y, Fujimoto H, Ezaki T, Butler RInt J Syst Evol Microbiol10.1099/ijs.0.025478-02010Aged, Aged, 80 and over, Bacterial Proteins/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Female, Humans, Male, Middle Aged, Molecular Sequence Data, Mycobacterium/*classification/genetics/growth & development/*isolation & purification, Mycobacterium Infections/*microbiology, Phylogeny, RNA, Ribosomal, 16S/geneticsPathogenicity
Metabolism23264129Mycobacterium kansasii-induced death of murine macrophages involves endoplasmic reticulum stress responses mediated by reactive oxygen species generation or calpain activation.Lim YJ, Choi HH, Choi JA, Jeong JA, Cho SN, Lee JH, Park JB, Kim HJ, Song CHApoptosis10.1007/s10495-012-0792-42013Animals, Calpain/*metabolism, Caspases/metabolism, Cell Line, Endoplasmic Reticulum Stress/*physiology, Enzyme Activation, Female, Macrophages/microbiology/*pathology, Mice, Mice, Inbred C57BL, Mycobacterium Infections, Nontuberculous/*physiopathology, Mycobacterium kansasii/*pathogenicity, Reactive Oxygen Species/metabolism, VirulenceStress
Genetics25716827Insights on the emergence of Mycobacterium tuberculosis from the analysis of Mycobacterium kansasii.Wang J, McIntosh F, Radomski N, Dewar K, Simeone R, Enninga J, Brosch R, Rocha EP, Veyrier FJ, Behr MAGenome Biol Evol10.1093/gbe/evv0352015Animals, *Evolution, Molecular, Female, Genome, Bacterial, Genomics, Mice, Inbred C57BL, Mycobacterium kansasii/classification/*genetics, Mycobacterium tuberculosis/classification/*genetics/pathogenicity, Phenotype, Phylogeny, Virulence/geneticsPhylogeny
Metabolism25893968MKAN27435 is required for the biosynthesis of higher subclasses of lipooligosaccharides in Mycobacterium kansasii.Nataraj V, Pang PC, Haslam SM, Veerapen N, Minnikin DE, Dell A, Besra GS, Bhatt APLoS One10.1371/journal.pone.01228042015Gene Knockout Techniques, Glycosyltransferases/deficiency/genetics/*metabolism, Lipopolysaccharides/*biosynthesis/chemistry, Multigene Family/genetics, Mutation, Mycobacterium kansasii/enzymology/genetics/*metabolism, Open Reading Frames/geneticsEnzymology
Genetics27375559Comparative Genomics and Proteomic Analysis of Four Non-tuberculous Mycobacterium Species and Mycobacterium tuberculosis Complex: Occurrence of Shared Immunogenic Proteins.Gcebe N, Michel A, Gey van Pittius NC, Rutten VFront Microbiol10.3389/fmicb.2016.007952016
27681720The Structure-Antimicrobial Activity Relationships of a Promising Class of the Compounds Containing the N-Arylpiperazine Scaffold.Malik I, Csollei J, Jampilek J, Stanzel L, Zadrazilova I, Hosek J, Pospisilova S, Cizek A, Coffey A, O'Mahony JMolecules10.3390/molecules211012742016
Enzymology29535391Molecular typing of Mycobacterium kansasii using pulsed-field gel electrophoresis and a newly designed variable-number tandem repeat analysis.Bakula Z, Brzostek A, Borowka P, Zaczek A, Szulc-Kielbik I, Podpora A, Parniewski P, Strapagiel D, Dziadek J, Proboszcz M, Bielecki J, van Ingen J, Jagielski TSci Rep10.1038/s41598-018-21562-z2018Bacterial Typing Techniques/methods, DNA, Bacterial/genetics, Electrophoresis, Gel, Pulsed-Field, *Minisatellite Repeats, Molecular Typing/*methods, Mycobacterium kansasii/*classification/geneticsPhylogeny
34504483A Murine Model of Mycobacterium kansasii Infection Reproducing Necrotic Lung Pathology Reveals Considerable Heterogeneity in Virulence of Clinical Isolates.Mussi VO, Simao TLBV, Almeida FM, Machado E, de Carvalho LD, Calixto SD, Sales GAM, Carvalho ECQ, Vasconcellos SEG, Catanho M, Suffys PN, Lasunskaia EBFront Microbiol10.3389/fmicb.2021.7184772021
Pathogenicity34933140An overview of drugs for the treatment of Mycobacterium kansasii pulmonary disease.Srivastava S, Pasipanodya JG, Heysell SK, Boorgula GD, Gumbo T, McShane PJ, Philley JVJ Glob Antimicrob Resist10.1016/j.jgar.2021.12.0102021Anti-Bacterial Agents/pharmacology/therapeutic use, Humans, *Lung Diseases/drug therapy/microbiology, Microbial Sensitivity Tests, Moxifloxacin/pharmacology, *Mycobacterium kansasii
Pathogenicity35976006Omadacycline Pharmacokinetics/Pharmacodynamics in the Hollow Fiber System Model and Potential Combination Regimen for Short Course Treatment of Mycobacterium kansasii Pulmonary Disease.Singh S, Gumbo T, Boorgula GD, Shankar P, Heysell SK, Srivastava SAntimicrob Agents Chemother10.1128/aac.00687-222022Anti-Bacterial Agents/therapeutic use, Humans, *Lung Diseases/microbiology, Microbial Sensitivity Tests, Moxifloxacin/pharmacology/therapeutic use, *Mycobacterium kansasii, TetracyclinesEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11560Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44162)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44162
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36241Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16514
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77792Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44426.1StrainInfo: A central database for resolving microbial strain identifiers
120378Curators of the CIPCollection of Institut Pasteur (CIP 104589)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104589