Strain identifier

BacDive ID: 8379

Type strain: Yes

Species: Mycobacterium aurum

Strain Designation: 358, 14 121 0001

Strain history: CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 121 0001 <- ATCC <- M. Tsukamura: strain 358

NCBI tax ID(s): 1791 (species)

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General

@ref: 11447

BacDive-ID: 8379

DSM-Number: 43999

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Mycobacterium aurum 358 is an aerobe, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 1791
  • Matching level: species

strain history

@refhistory
11447<- J.R. Andreesen, Univ. Göttingen <- ATCC <- M. Tsukamura, 358
67770M. Tsukamura 15006.
116641CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 121 0001 <- ATCC <- M. Tsukamura: strain 358

doi: 10.13145/bacdive8379.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium aurum
  • full scientific name: Mycobacterium aurum Tsukamura 1966 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium aurum

@ref: 11447

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium aurum

full scientific name: Mycobacterium aurum Tsukamura 1966 emend. Nouioui et al. 2018

strain designation: 358, 14 121 0001

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.885
69480100positive
116641norod-shaped

multimedia

@refmultimedia contentintellectual property rightscaption
11447https://www.dsmz.de/microorganisms/photos/DSM_43999-1.jpg© Leibniz-Institut DSMZ
11447https://www.dsmz.de/microorganisms/photos/DSM_43999.jpg© Leibniz-Institut DSMZMedium 645 37°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11447MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
11447LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
41184MEDIUM 55 - for Mycobacteriumyes
116641CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
11447positivegrowth37mesophilic
41184positivegrowth37mesophilic
63730positivegrowth30mesophilic
67770positivegrowth37mesophilic
116641positivegrowth25-37mesophilic
116641nogrowth10psychrophilic
116641nogrowth41thermophilic
116641nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
63730aerobe
116641obligate aerobe

spore formation

@refspore formationconfidence
69481no94
69480no99.801

halophily

@refsaltgrowthtested relationconcentration
116641NaClpositivegrowth0-4 %
116641NaClnogrowth6 %
116641NaClnogrowth8 %
116641NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
1166414853esculin+hydrolysis
11664117632nitrate+reduction
11664116301nitrite-reduction
11664117632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11664135581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11664115688acetoin-
11664117234glucose-

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
116641oxidase-
116641beta-galactosidase-3.2.1.23
116641alcohol dehydrogenase-1.1.1.1
116641gelatinase-
116641amylase-
116641DNase-
116641caseinase-3.4.21.50
116641catalase+1.11.1.6
116641gamma-glutamyltransferase+2.3.2.2
116641lecithinase-
116641lysine decarboxylase-4.1.1.18
116641ornithine decarboxylase-4.1.1.17
116641phenylalanine ammonia-lyase-4.3.1.24
116641tryptophan deaminase-
116641urease+3.5.1.5
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382alpha-chymotrypsin-3.4.21.1
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
11447--+/-+-+-+/--------+/-----
116641-+++++-++-+---------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
11447---------+-+--+--+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116641++------------------++----------++---------------+----------+------+----+-----------+---+-------+-+

Isolation, sampling and environmental information

isolation

@refsample type
11447soil
63730Soil
67770Soil
116641Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
114471Risk group (German classification)
1166411Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium aurum strain ATCC 23366 16S ribosomal RNA gene, partial sequenceFJ1722981453ena1791
20218M.aurum 16S ribosomal RNAM295581349ena1791
20218M.aurum 16S ribosomal RNA, partX555951458ena1791
20218Mycobacterium aurum 16S ribosomal RNA gene, partial sequenceAY438068540ena1791

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium aurum NCTC10437GCA_900637195completencbi1791
66792Mycolicibacterium aurum strain NCTC104371791.6completepatric1791
66792Mycolicibacterium aurum NCTC 104372740892265draftimg1791
67770Mycolicibacterium aurum NCTC 10437GCA_001049355scaffoldncbi1791
66792Mycolicibacterium aurum DSM 43999GCA_025821345scaffoldncbi1791

GC content

  • @ref: 67770
  • GC-content: 67.5
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno94no
flagellatedno97.362no
gram-positiveyes89.805no
anaerobicno99.295yes
aerobicyes92.247yes
halophileno90.983no
spore-formingno78.507no
thermophileno99.748no
glucose-utilyes84.72yes
motileno90.536no
glucose-fermentno90.561yes

External links

@ref: 11447

culture collection no.: DSM 43999, ATCC 23366, JCM 6366, NCTC 10437, CCUG 70546, LMG 19255, CIP 104465, KCTC 9955, NRRL B-4037, Tsukamura 358, CCUG 37666, HAMBI 2275

straininfo link

  • @ref: 77778
  • straininfo: 13221

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism2306483Mycolic acid synthesis by Mycobacterium aurum cell-free extracts.Lacave C, Laneelle MA, Laneelle GBiochim Biophys Acta10.1016/0005-2760(90)90159-u1990Acetates/metabolism, Buffers, Cell-Free System, Chemical Phenomena, Chemistry, Mycobacterium/*metabolism/ultrastructure, Mycolic Acids/*isolation & purification, Time FactorsEnzymology
Phylogeny12508859Classification of a polycyclic aromatic hydrocarbon-metabolizing bacterium, Mycobacterium sp. strain PYR-1, as Mycobacterium vanbaalenii sp. nov.Khan AA, Kim SJ, Paine DD, Cerniglia CEInt J Syst Evol Microbiol10.1099/00207713-52-6-19972002Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Environmental Pollutants/metabolism, Fatty Acids/analysis, Geologic Sediments/microbiology, Mycobacterium/*classification/genetics/isolation & purification/*metabolism, Mycolic Acids/analysis, Petroleum/metabolism, Phenotype, Phylogeny, Polycyclic Aromatic Hydrocarbons/*metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityMetabolism
Pathogenicity24786571Antibacterial activities of plants from Central Africa used traditionally by the Bakola pygmies for treating respiratory and tuberculosis-related symptoms.Fomogne-Fodjo MC, Van Vuuren S, Ndinteh DT, Krause RW, Olivier DKJ Ethnopharmacol10.1016/j.jep.2014.04.0322014Africa, Central, Anti-Bacterial Agents/isolation & purification/*pharmacology, Bacteria/drug effects, Cameroon, Ethnopharmacology, Medicine, African Traditional/methods, Microbial Sensitivity Tests, Mycobacterium/*drug effects, Phytotherapy/methods, Plant Extracts/*pharmacology, Plants, Medicinal/*chemistry, Respiratory Tract Infections/drug therapy/microbiologyEnzymology
Pathogenicity28705154Phenotypic and genomic comparison of Mycobacterium aurum and surrogate model species to Mycobacterium tuberculosis: implications for drug discovery.Namouchi A, Cimino M, Favre-Rochex S, Charles P, Gicquel BBMC Genomics10.1186/s12864-017-3924-y2017*Drug Discovery, Drug Resistance, Bacterial/genetics, *Genomics, Molecular Sequence Annotation, Mycobacterium tuberculosis/*drug effects/*genetics/metabolism, *Phenotype, Species SpecificityGenetics
32650510Antimicrobial and Efflux Pump Inhibitory Activity of Carvotacetones from Sphaeranthus africanus Against Mycobacteria.Tran HT, Solnier J, Pferschy-Wenzig EM, Kunert O, Martin L, Bhakta S, Huynh L, Le TM, Bauer R, Bucar FAntibiotics (Basel)10.3390/antibiotics90703902020
34131377Synthesis, structural characterization and antimycobacterial evaluation of several halogenated non-nitro benzothiazinones.Madikizela B, Eckhardt T, Goddard R, Richter A, Lins A, Lehmann C, Imming P, Seidel RWMed Chem Res10.1007/s00044-021-02735-42021
Phylogeny34878372Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species.Cheng Y, Lei W, Wang X, Tian Z, Liu H, Yang J, Lu S, Lai XH, Pu J, Huang Y, Zhang S, Yang C, Lian X, Bai Y, Wan K, Wang S, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0050192021Animals, Antelopes/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Feces/microbiology, Mycobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TibetTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11447Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43999)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43999
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41184Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16376
63730Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 70546)https://www.ccug.se/strain?id=70546
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77778Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13221.1StrainInfo: A central database for resolving microbial strain identifiers
116641Curators of the CIPCollection of Institut Pasteur (CIP 104465)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104465