Strain identifier

BacDive ID: 8339

Type strain: Yes

Species: Mycobacterium lehmannii

Strain Designation: 40

Strain history: <- I. Tarnok, SN 1900 (Mycobacterium flavescens) <- J. Grange, Inst. of Pathology, London; 40

NCBI tax ID(s): 2048550 (species)

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General

@ref: 10876

BacDive-ID: 8339

DSM-Number: 43219

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, human pathogen

description: Mycobacterium lehmannii 40 is a facultative anaerobe, mesophilic, Gram-positive human pathogen of the family Mycobacteriaceae.

NCBI tax id

  • NCBI tax id: 2048550
  • Matching level: species

strain history

  • @ref: 10876
  • history: <- I. Tarnok, SN 1900 (Mycobacterium flavescens) <- J. Grange, Inst. of Pathology, London; 40

doi: 10.13145/bacdive8339.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium lehmannii
  • full scientific name: Mycobacterium lehmannii Nouioui et al. 2017

@ref: 10876

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium lehmannii

full scientific name: Mycobacterium lehmannii Nouioui et al. 2017

strain designation: 40

type strain: yes

Morphology

cell morphology

  • @ref: 65228
  • gram stain: positive
  • motility: no

colony morphology

  • @ref: 65228
  • colony color: yellow-orange
  • incubation period: 5 days
  • medium used: Middelbrook 7H10

multimedia

  • @ref: 10876
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43219.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10876MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
10876LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
65228Middelbrook 7H10yes
65228proteose peptone-meat extract-glycerol agaryes
65228glucose-yeast extract-malt extractyes
65228tryptic soy agaryes

culture temp

@refgrowthtypetemperaturerange
10876positivegrowth37mesophilic
65228positivegrowth28-37mesophilic
65228positiveoptimum37mesophilic

culture pH

  • @ref: 65228
  • ability: positive
  • type: growth
  • pH: 7

Physiology and metabolism

oxygen tolerance

  • @ref: 65228
  • oxygen tolerance: facultative anaerobe

halophily

  • @ref: 65228
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 4 %(w/v)

observation

@refobservation
65228Meso-diaminopimelicacid, arabinose, galactose, glucose and ribose are present in the whole-organism hydrolysates. The predominant menaquinone is MK-9(H 2 ).
65228Contains dicarboxy mycolic acids with 61-64 carbon atoms.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6522815588D-malate+assimilation
6522816651(S)-lactate-assimilation
6522815589L-malate+assimilation
65228167632-oxobutanoate-assimilation
65228309162-oxoglutarate+assimilation
65228370543-hydroxybutyrate+assimilation
65228739183-O-methyl-D-glucose-assimilation
65228181014-hydroxyphenylacetic acid-assimilation
6522830089acetate+assimilation
6522813705acetoacetate+assimilation
65228645522-hydroxybutyrate+assimilation
6522871422beta-gentiobiose-assimilation
6522873706bromosuccinate-assimilation
6522827689decanoate+assimilation
6522817057cellobiose+assimilation
6522816947citrate+assimilation
6522818333D-arabitol+assimilation
6522829990D-aspartate-assimilation
6522815824D-fructose+assimilation
6522878697D-fructose 6-phosphate+assimilation
6522828847D-fucose+assimilation
6522812936D-galactose-assimilation
6522818024D-galacturonic acid-assimilation
6522830612D-glucarate-assimilation
652288391D-gluconate+assimilation
6522817634D-glucose+assimilation
6522814314D-glucose 6-phosphate-assimilation
6522815748D-glucuronate+assimilation
6522816899D-mannitol+assimilation
6522816024D-mannose+assimilation
6522816523D-serine+assimilation
6522817924D-sorbitol+assimilation
6522832528turanose-assimilation
6522823652dextrin-assimilation
6522816537galactarate-assimilation
6522816865gamma-aminobutyric acid-assimilation
652285291gelatin-assimilation
6522832323glucuronamide-assimilation
6522817754glycerol+assimilation
6522870744glycine-proline+assimilation
6522817596inosine-assimilation
6522816977L-alanine-assimilation
6522816467L-arginine-assimilation
6522829991L-aspartate-assimilation
6522818287L-fucose-assimilation
6522817464L-galactonic acid gamma-lactone-assimilation
6522829985L-glutamate+assimilation
6522815971L-histidine-assimilation
6522818183L-pyroglutamic acid-assimilation
6522862345L-rhamnose-assimilation
6522817115L-serine-assimilation
6522817716lactose-assimilation
6522817306maltose-assimilation
6522828053melibiose-assimilation
6522874611methyl (R)-lactate-assimilation
65228320055methyl beta-D-glucopyranoside-assimilation
6522851850methyl pyruvate+assimilation
6522817268myo-inositol-assimilation
6522863154N-acetyl-beta-D-mannosamine-assimilation
6522828037N-acetylgalactosamine-assimilation
65228506227N-acetylglucosamine-assimilation
6522835418n-acetylneuraminate-assimilation
6522817632nitrate+reduction
6522817309pectin-assimilation
6522817272propionate+assimilation
6522826490quinate-assimilation
6522816634raffinose-assimilation
6522817814salicin+assimilation
6522817164stachyose-assimilation
6522817992sucrose-assimilation
6522827082trehalose+assimilation
6522853423tween 40+degradation
6522853426tween 80-degradation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
6522871321fusidateyesyes
652286472lincomycinyesyes
6522850694minocyclineyesyes
6522875273niaproofyesyes
6522845735troleandomycinyesyes
6522875229sodium bromateyesyes
6522832735guanidinium chlorideyesyes
6522875198tetrazolium blueyesyes
6522875193tetrazolium violetyesyes
6522828001vancomycinyesyes
65228161680aztreonamyesyes
6522848607lithium chlorideyesyes
65228100147nalidixic acidyesyes
6522829673rifamycin svyesyes
6522875248potassium telluriteyesyes
6522862965sodium formateyesyes
652281 % sodium lactateyesyes

enzymes

@refvalueactivityec
65228arylsulfatase+3.1.6.1
65228esterase (C 4)+
65228alkaline phosphatase+3.1.3.1
65228alpha-glucosidase+3.2.1.20
65228beta-glucosidase+3.2.1.21
65228catalase+1.11.1.6
65228urease+3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    65228C16:017.4
    65228C16:1ω6c7.5
    65228C18:02.9
    65228C18:1ω9c8.9
    65228C18:2ω6,9c3.3
    65228C10Me-C18:06.1
    65228C14:02.2
    65228C17:1ω7c / C17:1ω6c18.5
    65228unknown31.319
  • type of FA analysis: whole cell analysis
  • incubation medium: MD7H10 broth
  • incubation temperature: 37
  • incubation time: 7
  • software version: Sherlock 4.5
  • library/peak naming table: Myco5
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 10876
  • country: United Kingdom
  • origin.country: GBR
  • continent: Europe

Safety information

risk assessment

  • @ref: 10876
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 10876
  • description: Mycobacterium lehmannii strain CECT 8763 16S ribosomal RNA gene, partial sequence
  • accession: KY933300
  • length: 1530
  • database: ena
  • NCBI tax ID: 2048550

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium lehmannii CECT 8763GCA_002245535contigncbi2048550
66792Mycobacterium sp. CECT 87632015070.3wgspatric2048550

GC content

@refGC-contentmethod
1087666.7sequence analysis
6522866.7genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.916yes
gram-positiveyes87.556yes
anaerobicno99.393no
aerobicyes89.69no
halophileno88.944no
spore-formingno87.007no
glucose-utilyes85.9yes
flagellatedno98.729yes
thermophileno99.298yes
glucose-fermentno91.724no

External links

@ref: 10876

culture collection no.: DSM 43219, CECT 8763, SN 1900

straininfo link

  • @ref: 77741
  • straininfo: 50205

literature

  • topic: Phylogeny
  • Pubmed-ID: 29058645
  • title: Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.
  • authors: Nouioui I, Sangal V, Carro L, Teramoto K, Jando M, Montero-Calasanz MDC, Igual JM, Sutcliffe I, Goodfellow M, Klenk HP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002350
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Multilocus Sequence Typing, Mycobacterium/*classification/isolation & purification, Mycolic Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10876Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43219)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43219
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
65228Imen Nouioui, Vartul Sangal, Lorena Carro, Kanae Teramoto, Marlen Jando, Maria del Carmen Montero-Calasanz, José Mariano Igual, Iain Sutcliffe, Michael Goodfellow, Hans-Peter Klenk10.1099/ijsem.0.002350Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.IJSEM 67: 4948-4955 201729058645
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77741Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50205.1StrainInfo: A central database for resolving microbial strain identifiers