Strain identifier

BacDive ID: 8087

Type strain: No

Species: Acinetobacter junii

Strain Designation: 17AO4

Strain history: CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 17A04, Acinetobacter grimontii

NCBI tax ID(s): 1217666 (strain), 40215 (species)

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General

@ref: 5643

BacDive-ID: 8087

DSM-Number: 14968

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative

description: Acinetobacter junii 17AO4 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from Wastewater treatment plant.

NCBI tax id

NCBI tax idMatching level
1217666strain
40215species

strain history

@refhistory
5643<- P. Kämpfer; 17AO4 <- G. Knight
421352002, P. Kämpfer, Univ., Giessen, Germany: strain 17A04, Acinetobacter grimontii
120366CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 17A04, Acinetobacter grimontii

doi: 10.13145/bacdive8087.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter junii
  • full scientific name: Acinetobacter junii Bouvet and Grimont 1986

  • synonyms

    • @ref: 20215
    • synonym: Acinetobacter grimontii

@ref: 5643

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter junii

full scientific name: Acinetobacter junii Bouvet and Grimont 1986

strain designation: 17AO4

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.885
120366negativerod-shapedno

colony morphology

  • @ref: 120366

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5643CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
5643TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
42135MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120366CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5643positivegrowth30mesophilic
42135positivegrowth30mesophilic
58980positivegrowth30-42
120366positivegrowth25-41
120366nogrowth10psychrophilic
120366nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58980aerobe
120366obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.779

halophily

@refsaltgrowthtested relationconcentration
120366NaClpositivegrowth0-4 %
120366NaClnogrowth6 %
120366NaClnogrowth8 %
120366NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12036616947citrate+carbon source
12036617632nitrate-reduction
12036616301nitrite-reduction
12036617234glucose-degradation
12036617632nitrate-respiration

antibiotic resistance

  • @ref: 120366
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12036635581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
12036615688acetoin-
12036617234glucose-

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
120366oxidase-
120366beta-galactosidase-3.2.1.23
120366gelatinase-
120366catalase+1.11.1.6
120366gamma-glutamyltransferase-2.3.2.2
120366urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120366--++-+----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5643---------------+--+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120366------------------------------------------------++---+-------------------+--++-++---------++----+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
5643Wastewater treatment plantVictoriaAustraliaAUSAustralia and Oceania
58980Activated sludge (Bendigo waste water treatment)VictoriaAustraliaAUSAustralia and Oceania1991
120366Environment, Bendigo waste water treatment plantVictoriaAustraliaAUSAustralia and Oceania1991

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Wastewater
#Engineered#Waste#Water treatment plant

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56432Risk group (German classification)
1203661Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acinetobacter grimontii strain DSM14968 16S ribosomal RNA gene, partial sequenceEF611411452ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 01HE651881707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 02HE651882713ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 03HE651883707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 18HE651884728ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 19HE651885713ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 20HE651886728ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 21HE651887728ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 22HE651888728ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 23HE651889707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 24HE651890707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 25HE651891728ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 26HE651892707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 28HE651893728ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 29HE651894713ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 30HE651895728ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 31HE651896728ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 33HE651897707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 34HE651898707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 35HE651899713ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 36HE651900707ena40215
20218Acinetobacter junii genomic DNA containing 16S-23S IGS, strain DSM 14968, clone 37HE651901707ena40215
20218Acinetobacter junii genomic DNA containing partial 16S-23S IGS, strain DSM 14968, clone 38HE651902706ena40215
5643Acinetobacter grimontii 16S ribosomal RNA gene, partial sequenceAF5098281325ena40215

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter junii CIP 107470 = MTCC 11364GCA_000368665scaffoldncbi1217666
66792Acinetobacter junii CIP 107470 = MTCC 11364GCA_000430225contigncbi1217666
66792Acinetobacter junii CIP 1074701217666.3wgspatric1217666
66792Acinetobacter junii CIP 1074701217666.5wgspatric1217666
66792Acinetobacter junii CIP 1074702534682023draftimg1217666

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno90.208no
flagellatedno92.303no
gram-positiveno98.178no
anaerobicno99.106no
aerobicyes94.902yes
halophileno89.394no
spore-formingno96.371no
thermophileno99.87yes
glucose-utilno85.367yes
glucose-fermentno91.605yes

External links

@ref: 5643

culture collection no.: CCUG 50767, DSM 14968, CIP 107470, CCM 7198

straininfo link

  • @ref: 77514
  • straininfo: 113569

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12892111Seven novel species of Acinetobacter isolated from activated sludge.Carr EL, Kampfer P, Patel BKC, Gurtler V, Seviour RJInt J Syst Evol Microbiol10.1099/ijs.0.02486-02003Acinetobacter/*classification/genetics/*isolation & purification, Base Sequence, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology, Terminology as Topic, VictoriaGenetics
Phylogeny18398198Reclassification of Acinetobacter grimontii Carr et al. 2003 as a later synonym of Acinetobacter junii Bouvet and Grimont 1986.Vaneechoutte M, De Baere T, Nemec A, Musilek M, van der Reijden TJ, Dijkshoorn LInt J Syst Evol Microbiol10.1099/ijs.0.65129-02008Acinetobacter/*classification/*genetics/metabolism, Amplified Fragment Length Polymorphism Analysis, Arginine/metabolism, DNA, Bacterial/genetics, Genes, Bacterial, Glutamic Acid/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species Specificity, Terminology as TopicMetabolism
Phylogeny25801684The impact of horizontal gene transfer on targeting the internal transcribed spacer region (ITS) to identify Acinetobacter junii strains.Maslunka C, Gurtler V, Seviour RJJ Appl Microbiol10.1111/jam.128002015Acinetobacter/classification/*genetics/isolation & purification, Base Sequence, DNA, Intergenic/*genetics, *Gene Transfer, Horizontal, Genetic Variation, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Reproducibility of ResultsGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5643Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14968)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14968
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42135Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4909
58980Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 50767)https://www.ccug.se/strain?id=50767
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77514Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID113569.1StrainInfo: A central database for resolving microbial strain identifiers
120366Curators of the CIPCollection of Institut Pasteur (CIP 107470)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107470