Strain identifier

BacDive ID: 8074

Type strain: No

Species: Acinetobacter johnsonii

Strain Designation: 5BO2, 5B02

Strain history: CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 5B02

NCBI tax ID(s): 472 (species)

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General

@ref: 5640

BacDive-ID: 8074

DSM-Number: 14965

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Acinetobacter johnsonii 5BO2 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from Wastewater treatment plant.

NCBI tax id

  • NCBI tax id: 472
  • Matching level: species

strain history

@refhistory
5640<- P. Kämpfer; 5BO2 <- G. Knight
331322002, P. Kämpfer, Univ., Giessen, Germany: strain 5B02
120357CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain 5B02

doi: 10.13145/bacdive8074.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter johnsonii
  • full scientific name: Acinetobacter johnsonii Bouvet and Grimont 1986

@ref: 5640

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter johnsonii

full scientific name: Acinetobacter sp.

strain designation: 5BO2, 5B02

type strain: no

Morphology

cell morphology

  • @ref: 120357
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 120357

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5640CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
5640TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
33132MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120357CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5640positivegrowth30mesophilic
33132positivegrowth30mesophilic
120357positivegrowth10-37
120357nogrowth41thermophilic
120357nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120357
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120357NaClpositivegrowth0-4 %
120357NaClnogrowth6 %
120357NaClnogrowth8 %
120357NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12035717632nitrate-reduction
12035716301nitrite-reduction
12035717234glucose-degradation
12035717632nitrate-respiration

antibiotic resistance

  • @ref: 120357
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12035735581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
12035715688acetoin-
12035717234glucose-

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
120357oxidase-
120357beta-galactosidase-3.2.1.23
120357alcohol dehydrogenase-1.1.1.1
120357gelatinase-
120357catalase+1.11.1.6
120357gamma-glutamyltransferase-2.3.2.2
120357lysine decarboxylase-4.1.1.18
120357ornithine decarboxylase-4.1.1.17
120357urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120357-+++-+----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5640---------------+-++/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120357-------------------------------------------------+---+-+-------------------+++-++---------++++--+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5640Wastewater treatment plantVictoriaAustraliaAUSAustralia and Oceania
120357Environment, Bendigo waste water treatment plantVictoriaAustraliaAUSAustralia and Oceania1991

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Wastewater
#Engineered#Waste#Water treatment plant

Safety information

risk assessment

  • @ref: 120357
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5640
  • description: Acinetobacter johnsonii 16S ribosomal RNA gene, partial sequence
  • accession: AF509831
  • length: 1325
  • database: ena
  • NCBI tax ID: 40214

External links

@ref: 5640

culture collection no.: DSM 14965, CIP 107467

straininfo link

  • @ref: 77501
  • straininfo: 113566

Reference

@idauthorscataloguedoi/urltitle
5640Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14965)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14965
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33132Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4906
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
77501Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID113566.1StrainInfo: A central database for resolving microbial strain identifiers
120357Curators of the CIPCollection of Institut Pasteur (CIP 107467)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107467