Strain identifier

BacDive ID: 8048

Type strain: Yes

Species: Rugosimonospora acidiphila

Strain Designation: Delta1, ID 143706

Strain history: DSM 45227 <-- P. Monciardini strain Delta1 <-- L. Cavaletti.

NCBI tax ID(s): 556531 (species)

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General

@ref: 15806

BacDive-ID: 8048

DSM-Number: 45227

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Rugosimonospora acidiphila Delta1 is an aerobe, spore-forming, mesophilic bacterium that was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 556531
  • Matching level: species

strain history

@refhistory
15806<- P. Monciardini, Vicuron Pharmaceuticals, Gerenzano, Italy; Delta1 <- L. Cavaletti
67770DSM 45227 <-- P. Monciardini strain Delta1 <-- L. Cavaletti.

doi: 10.13145/bacdive8048.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Rugosimonospora
  • species: Rugosimonospora acidiphila
  • full scientific name: Rugosimonospora acidiphila Monciardini et al. 2009

@ref: 15806

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Rugosimonospora

species: Rugosimonospora acidiphila

full scientific name: Rugosimonospora acidiphila Monciardini et al. 2009

strain designation: Delta1, ID 143706

type strain: yes

Morphology

cell morphology

  • @ref: 29286
  • gram stain: positive
  • motility: no

colony morphology

  • @ref: 19924
  • colony color: Beige
  • incubation period: 10-14 days
  • medium used: ISP 2

multicellular morphology

  • @ref: 19924
  • forms multicellular complex: no
  • medium name: ISP 2

pigmentation

  • @ref: 29286
  • production: yes

multimedia

  • @ref: 15806
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45227.jpg
  • caption: Medium 65 pH5.5 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15806TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
15806BTT MEDIUM (DSMZ Medium 1109)yeshttps://mediadive.dsmz.de/medium/1109Name: BTT MEDIUM (DSMZ Medium 1109) Composition: Agar 20.0 g/l Glucose 10.0 g/l Casitone 2.0 g/l Meat extract 1.0 g/l Yeast extract 1.0 g/l Distilled water
19924ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
15806GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
15806ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15806positivegrowth28mesophilic
19924positiveoptimum28mesophilic
29286positivegrowth17-32
29286positiveoptimum25mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepH
29286positivegrowth4.5-7.2
29286positiveoptimum5.5

Physiology and metabolism

oxygen tolerance

  • @ref: 29286
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29286
  • spore formation: yes

observation

@refobservation
29286aggregates in clumps
67770quinones: MK-9(H8), MK-9(H4), MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2928617754glycerol+carbon source
2928637684mannose+carbon source
2928618222xylose+carbon source

metabolite production

  • @ref: 29286
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
29286gelatinase+
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19924-+++-----+++--+++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15806forest soilGerenzanoItalyITAEurope
67770Temperate forest soilGerenzanoItalyITAEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 15806
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15806
  • description: Rugosimonospora acidiphila partial 16S rRNA gene, type strain NBRC 104874T
  • accession: FM208261
  • length: 1438
  • database: ena
  • NCBI tax ID: 556531

GC content

@refGC-contentmethod
1580672.7
6777072.7high performance liquid chromatography (HPLC)

External links

@ref: 15806

culture collection no.: DSM 45227, JCM 18304, NBRC 104874

straininfo link

  • @ref: 77476
  • straininfo: 397151

literature

  • topic: Phylogeny
  • Pubmed-ID: 19625428
  • title: Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov.
  • authors: Monciardini P, Cavaletti L, Ranghetti A, Schumann P, Rohde M, Bamonte R, Sosio M, Mezzelani A, Donadio S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.010231-0
  • year: 2009
  • mesh: DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Micromonosporaceae/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15806Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45227)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45227
19924Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45227.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29286Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2570228776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
77476Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID397151.1StrainInfo: A central database for resolving microbial strain identifiers