Strain identifier

BacDive ID: 7965

Type strain: Yes

Species: Micromonospora aurantiaca

Strain history: VKM Ac-1936 <-- INA 9442.

NCBI tax ID(s): 644283 (strain), 47850 (species)

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General

@ref: 11286

BacDive-ID: 7965

DSM-Number: 43813

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Micromonospora aurantiaca DSM 43813 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

NCBI tax idMatching level
644283strain
47850species

strain history

@refhistory
11286<- NRRL <- ATCC <- G.F. Gauze, INA
67770KCC A-0232 <-- BKM A-613 <-- LIA 0741.
67770VKM Ac-1936 <-- INA 9442.

doi: 10.13145/bacdive7965.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Micromonospora
  • species: Micromonospora aurantiaca
  • full scientific name: Micromonospora aurantiaca Sveshnikova et al. 1969 (Approved Lists 1980)

@ref: 11286

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Micromonospora

species: Micromonospora aurantiaca

full scientific name: Micromonospora aurantiaca Sveshnikova et al. 1969

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no92.301
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
19595Bright orange10-14 daysISP 2
19595Bright orange10-14 daysISP 3
19595Bright orange10-14 daysISP 4
19595Bright orange10-14 daysISP 5
19595Bright orange10-14 daysISP 6
19595Bright orange10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
19595noISP 2
19595noISP 3
19595noISP 4
19595yesISP 5Aerial MyceliumWhite
19595noISP 6
19595noISP 7

multimedia

@refmultimedia contentintellectual property rightscaption
11286https://www.dsmz.de/microorganisms/photos/DSM_43813-1.jpg© Leibniz-Institut DSMZ
11286https://www.dsmz.de/microorganisms/photos/DSM_43813.jpg© Leibniz-Institut DSMZMedium 553 37°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11286GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19595ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19595ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19595ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19595ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19595ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19595ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11286N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium554.pdf

culture temp

@refgrowthtypetemperaturerange
19595positiveoptimum28mesophilic
11286positivegrowth37mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480yes100

halophily

  • @ref: 19595
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1959517234glucose+/-
1959522599arabinose+/-
1959517992sucrose+/-
1959518222xylose+/-
1959517268myo-inositol+/-
1959529864mannitol+/-
1959528757fructose+/-
1959526546rhamnose+/-
1959516634raffinose+/-
1959562968cellulose+/-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase+4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19595+++++-++-++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.country
11286soil
67770SoilRussiaRUS

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_1923.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_404;97_1111;98_1509;99_1923&stattab=map
  • Last taxonomy: Micromonospora
  • 16S sequence: X92604
  • Sequence Identity:
  • Total samples: 804
  • soil counts: 470
  • aquatic counts: 188
  • animal counts: 133
  • plant counts: 13

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
112861Risk group (German classification)
195951Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Micromonospora aurantiaca DSM 43813 16S-23S ribosomal RNA intergenic spacerAY371889364ena47850
11286M.aurantiaca 16S rRNA geneX926041476ena47850

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Micromonospora aurantiaca ATCC 27029GCA_000145235completencbi644283
66792Micromonospora aurantiaca ATCC 27029644283.4completepatric644283
66792Micromonospora aurantiaca strain ATCC 2702947850.5wgspatric47850
66792Micromonospora aurantiaca ATCC 270292869041381draftimg644283
66792Micromonospora aurantiaca ATCC 27029648028042completeimg644283
67770Micromonospora aurantiaca ATCC 27029GCA_003721415contigncbi47850

GC content

@refGC-contentmethod
6777072.82genome sequence analysis
6777071.6high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno94.04no
flagellatedno98.098no
gram-positiveyes92.08no
anaerobicno99.413no
aerobicyes94.859no
halophileno94.631no
spore-formingyes95.822no
thermophileno98.657yes
glucose-utilyes90.637no
glucose-fermentno92.766no

External links

@ref: 11286

culture collection no.: DSM 43813, ATCC 27029, CBS 129.76, IFO 16155, IMET 8216, INA 9442, JCM 10878, KCC A-0232, NBRC 16155, NRRL B-16091, BCRC 12538, CECT 3289, CGMCC 4.2103, CGMCC 4.5705, IFO 16125, MTCC 1047, NBRC 16125, VKM Ac-1936, IFO 14068, JCM 3232, VKM Ac-613, NBRC 14068

straininfo link

  • @ref: 77399
  • straininfo: 92925

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19656925Micromonospora marina sp. nov., isolated from sea sand.Tanasupawat S, Jongrungruangchok S, Kudo TInt J Syst Evol Microbiol10.1099/ijs.0.014068-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/*microbiology, Micromonospora/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, ThailandMetabolism
Phylogeny20562246Micromonospora humi sp. nov., isolated from peat swamp forest soil.Songsumanus A, Tanasupawat S, Thawai C, Suwanborirux K, Kudo TInt J Syst Evol Microbiol10.1099/ijs.0.024281-02010DNA, Bacterial/genetics, Micromonospora/*classification/genetics/*isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Thailand, Trees/microbiologyGenetics
Phylogeny22523170Micromonospora sediminicola sp. nov., isolated from marine sediment.Supong K, Suriyachadkun C, Tanasupawat S, Suwanborirux K, Pittayakhajonwut P, Kudo T, Thawai CInt J Syst Evol Microbiol10.1099/ijs.0.041103-02012Bacterial Typing Techniques, Base Composition, Carbohydrates/analysis, DNA, Bacterial/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Micromonospora/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, ThailandGenetics
Phylogeny22523171Micromonospora maritima sp. nov., isolated from mangrove soil.Songsumanus A, Tanasupawat S, Igarashi Y, Kudo TInt J Syst Evol Microbiol10.1099/ijs.0.039180-02012Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Micromonospora/*classification/genetics/metabolism, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analysisGenetics
Phylogeny23667141Micromonospora schwarzwaldensis sp. nov., a producer of telomycin, isolated from soil.Gurovic MSV, Muller S, Domin N, Seccareccia I, Nietzsche S, Martin K, Nett MInt J Syst Evol Microbiol10.1099/ijs.0.051623-02013DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Germany, Micromonospora/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Peptides/*metabolism, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analysisGenetics
Phylogeny24257967Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora.Gao R, Liu C, Zhao J, Jia F, Yu C, Yang L, Wang X, Xiang WAntonie Van Leeuwenhoek10.1007/s10482-013-0074-32013Bacterial Typing Techniques, China, Cluster Analysis, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Micromonospora/*classification/genetics/*isolation & purification/physiology, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Biotechnology24428890Rational selection and engineering of exogenous principal sigma factor (sigma(HrdB)) to increase teicoplanin production in an industrial strain of Actinoplanes teichomyceticus.Wang H, Yang L, Wu K, Li GMicrob Cell Fact10.1186/1475-2859-13-102014Bacterial Proteins/classification/*genetics/metabolism, Evolution, Molecular, Genetic Engineering, *Industrial Microbiology, Micromonosporaceae/*genetics/*metabolism, Mutagenesis, Phylogeny, Sigma Factor/*genetics/metabolism, Teicoplanin/*biosynthesisPhylogeny
Metabolism25124041Allosteric regulation of a protein acetyltransferase in Micromonospora aurantiaca by the amino acids cysteine and arginine.Xu JY, You D, Leng PQ, Ye BCJ Biol Chem10.1074/jbc.M114.5790782014Acetylation, Acetyltransferases/genetics/*metabolism, Allosteric Regulation/physiology, Arginine/genetics/*metabolism, Bacterial Proteins/genetics/*metabolism, Coenzyme A Ligases/genetics/metabolism, Cysteine/genetics/*metabolism, Micromonospora/*enzymology/geneticsEnzymology
Phylogeny32915290Actinoplanes solisilvae sp. nov., Isolated from Birch Forest Soil.Ma Q, Zhang Q, Jiang X, Kong D, Han X, Xue H, Zhou Y, Zhang Y, Zhang W, Ruan ZCurr Microbiol10.1007/s00284-020-02192-z2020*Actinoplanes, Bacterial Typing Techniques, Betula, China, DNA, Bacterial/genetics, Forests, Micromonospora, Nucleic Acid Hybridization, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, Soil MicrobiologyTranscriptome
Biotechnology32922368Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis.Hu D, Sun C, Jin T, Fan G, Mok KM, Li K, Lee SMFront Microbiol10.3389/fmicb.2020.015402020
Phylogeny33174828Micromonospora fluminis sp. nov., isolated from mountain river sediment.Camacho Pozo MI, Wieme AD, Rodriguez Perez S, Llaurado Maury G, Peeters C, Snauwaert C, Lescaylle Veranes Y, Pena Zamora L, Schumann P, Vandamme PAInt J Syst Evol Microbiol10.1099/ijsem.0.0045542020Bacterial Typing Techniques, Base Composition, Cuba, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Micromonospora/*classification/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Vitamin K 2/chemistryTranscriptome
35855324Psi-Footprinting approach for the identification of protein synthesis inhibitor producers.Handel F, Kulik A, Wex KW, Berscheid A, Saur JS, Winkler A, Wibberg D, Kalinowski J, Brotz-Oesterhelt H, Mast YNAR Genom Bioinform10.1093/nargab/lqac0552022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11286Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43813)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43813
19595Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43813.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77399Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92925.1StrainInfo: A central database for resolving microbial strain identifiers