Strain identifier

BacDive ID: 7849

Type strain: Yes

Species: Asanoa ishikariensis

Strain Designation: 6432-C

Strain history: CIP <- 2002, IFO <- 1986, Kyowa Hakko Kogyo Co. Ltd.: strain 6432-C

NCBI tax ID(s): 137265 (species)

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General

@ref: 12037

BacDive-ID: 7849

DSM-Number: 44718

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Asanoa ishikariensis 6432-C is a spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 137265
  • Matching level: species

strain history

@refhistory
12037<- IFO <- K. Asano; 6432-C
67770IMSNU 22004 <-- Y. C. Hah <-- IFO 14551 <-- K. Asano 6432-C.
120284CIP <- 2002, IFO <- 1986, Kyowa Hakko Kogyo Co. Ltd.: strain 6432-C

doi: 10.13145/bacdive7849.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Asanoa
  • species: Asanoa ishikariensis
  • full scientific name: Asanoa ishikariensis Lee and Hah 2002

@ref: 12037

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Asanoa

species: Asanoa ishikariensis

full scientific name: Asanoa ishikariensis Lee and Hah 2002

strain designation: 6432-C

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no93.775
69480100positive
120284nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39903MEDIUM 116 - for Streptomyces, Nocardia, Streptosporangium and Mycobacterium chlorophenolicumyesDistilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g)
12037CZAPEK PEPTONE AGAR (DSMZ Medium 83)yesName: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled waterhttps://mediadive.dsmz.de/medium/83
12037TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
12037GPHF-MEDIUM (DSMZ Medium 553)yesName: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled waterhttps://mediadive.dsmz.de/medium/553
12037STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yesName: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled waterhttps://mediadive.dsmz.de/medium/252
120284CIP Medium 116yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=116

culture temp

@refgrowthtypetemperaturerange
39903positivegrowth30mesophilic
67770positivegrowth28mesophilic
12037positivegrowth28mesophilic
120284positivegrowth25-30mesophilic
120284nogrowth10psychrophilic
120284nogrowth37mesophilic
120284nogrowth41thermophilic
120284nogrowth45thermophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 100

halophily

@refsaltgrowthtested relationconcentration
120284NaClpositivegrowth0 %
120284NaClnogrowth2 %
120284NaClnogrowth4 %
120284NaClnogrowth6 %
120284NaClnogrowth8 %
120284NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-10(H8), MK-10(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12028416947citrate-carbon source
1202844853esculin+hydrolysis
120284606565hippurate+hydrolysis
12028417632nitrate-reduction
12028416301nitrite-reduction

metabolite production

  • @ref: 120284
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12028415688acetoin-
12028417234glucose-

enzymes

@refvalueactivityec
120284oxidase+
120284beta-galactosidase+3.2.1.23
120284alcohol dehydrogenase-1.1.1.1
120284gelatinase+
120284amylase+
120284DNase-
120284caseinase+3.4.21.50
120284catalase+1.11.1.6
120284tween esterase-
120284gamma-glutamyltransferase+2.3.2.2
120284lecithinase-
120284lipase-
120284lysine decarboxylase-4.1.1.18
120284ornithine decarboxylase-4.1.1.17
120284phenylalanine ammonia-lyase-4.3.1.24
120284protease-
120284tryptophan deaminase-
120284urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120284-+++-+----++----+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120284------------------+--------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
12037soilJapanJPNAsia
67770Soil
120284Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_77379.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_404;97_11524;98_18497;99_77379&stattab=map
  • Last taxonomy: Asanoa
  • 16S sequence: AJ294715
  • Sequence Identity:
  • Total samples: 44
  • soil counts: 41
  • aquatic counts: 3

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
120371Risk group (German classification)
1202841Risk group (French classification)

Sequence information

16S sequences

  • @ref: 12037
  • description: Catellatospora ishikariense partial 16S rRNA gene, strain IMSNU 22004
  • accession: AJ294715
  • length: 1508
  • database: ena
  • NCBI tax ID: 137265

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Asanoa ishikariensis NBRC 14551GCA_016862535contigncbi137265
66792Asanoa ishikariensis strain DSM 44718137265.3wgspatric137265
66792Asanoa ishikariensis strain NBRC 14551137265.4wgspatric137265
66792Asanoa ishikariensis DSM 447182690315652draftimg137265
67770Asanoa ishikariensis DSM 44718GCA_900107455contigncbi137265

GC content

@refGC-contentmethod
1203771.1
6777071.1high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes73no
motileno91.893no
gram-positiveyes85.81no
anaerobicno98.836no
halophileno93.159no
spore-formingyes89.038no
thermophileno96.045yes
glucose-utilyes89.809no
aerobicyes91.485no
flagellatedno98.446no
glucose-fermentno90.026no

External links

@ref: 12037

culture collection no.: DSM 44718, IFO 14551, IMSNU 22004, NBRC 14551, JCM 11895, CIP 107585

straininfo link

  • @ref: 77286
  • straininfo: 62332

literature

  • topic: Phylogeny
  • Pubmed-ID: 12054264
  • title: Proposal to transfer Catellatospora ferruginea and 'Catellatospora ishikariense' to Asanoa gen. nov. as Asanoa ferruginea comb. nov. and Asanoa ishikariensis sp. nov., with emended description of the genus Catellatospora.
  • authors: Lee SD, Hah YC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-3-967
  • year: 2002
  • mesh: Bacterial Typing Techniques, DNA, Ribosomal/analysis, Micromonosporaceae/chemistry/*classification/genetics/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
12037Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44718)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44718
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39903Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5036
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77286Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID62332.1StrainInfo: A central database for resolving microbial strain identifiers
120284Curators of the CIPCollection of Institut Pasteur (CIP 107585)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107585