Strain identifier

BacDive ID: 7848

Type strain: Yes

Species: Asanoa ferruginea

Strain history: CIP <- 2001, JCM <- IFO <- I. Kawamoto: strain 6257-C, Kyowa Hakko Cie

NCBI tax ID(s): 53367 (species)

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General

@ref: 11395

BacDive-ID: 7848

DSM-Number: 44099

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Asanoa ferruginea DSM 44099 is a mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 53367
  • Matching level: species

strain history

@refhistory
11395<- IFO <- I. Kawamoto, Kyowa Hakko Co., 6257-C
67770IFO 14496 <-- I. Kawamoto 6257-C.
119332CIP <- 2001, JCM <- IFO <- I. Kawamoto: strain 6257-C, Kyowa Hakko Cie

doi: 10.13145/bacdive7848.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Asanoa
  • species: Asanoa ferruginea
  • full scientific name: Asanoa ferruginea (Asano and Kawamoto 1986) Lee and Hah 2002

  • synonyms

    • @ref: 20215
    • synonym: Catellatospora ferruginea

@ref: 11395

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Asanoa

species: Asanoa ferruginea

full scientific name: Asanoa ferruginea (Asano and Kawamoto 1986) Lee and Hah 2002

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.724
69480100positive
119332nopositiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
19996Pure orange (2004)10-14 daysISP 2
19996Bright red orange (2008)10-14 daysISP 3
19996Colorless10-14 daysISP 4
19996Colorless10-14 daysISP 5
19996Pure orange (2004)10-14 daysISP 6
19996Pure orange (2004)10-14 daysISP 7

multicellular morphology

@refmedium nameforms multicellular complex
19996ISP 2
19996ISP 3no
19996ISP 4no
19996ISP 5no
19996ISP 6no
19996ISP 7no

multimedia

@refmultimedia contentintellectual property rightscaption
11395https://www.dsmz.de/microorganisms/photos/DSM_44099-1.jpg© Leibniz-Institut DSMZ
11395https://www.dsmz.de/microorganisms/photos/DSM_44099.jpg© Leibniz-Institut DSMZMedium 65 28°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11395GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19996ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19996ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19996ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19996ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19996ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19996ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
39635MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
11395N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
119332CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
19996positiveoptimum28mesophilic
11395positivegrowth28mesophilic
39635positivegrowth30mesophilic
67770positivegrowth28mesophilic
119332positivegrowth25-30mesophilic
119332nogrowth10psychrophilic
119332nogrowth37mesophilic
119332nogrowth41thermophilic
119332nogrowth45thermophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
19996no
69480yes100

halophily

@refsaltgrowthtested relationconcentration
19996NaClpositivegrowth0 %
119332NaClpositivegrowth0 %
119332NaClnogrowth2 %
119332NaClnogrowth4 %
119332NaClnogrowth6 %
119332NaClnogrowth8 %
119332NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-10(H8), MK-10(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1999617234glucose+/-
1999622599arabinose+/-
1999617992sucrose+/-
1999618222xylose+/-
1999617268myo-inositol+/-
1999629864mannitol+/-
1999628757fructose+/-
1999626546rhamnose+/-
1999616634raffinose+/-
1999662968cellulose+/-
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
11933216947citrate-carbon source
1193324853esculin+hydrolysis
119332606565hippurate+hydrolysis
11933217632nitrate-reduction
11933216301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11933235581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11933215688acetoin-
11933217234glucose-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
119332oxidase-
119332beta-galactosidase+3.2.1.23
119332alcohol dehydrogenase-1.1.1.1
119332gelatinase-
119332catalase+1.11.1.6
119332gamma-glutamyltransferase+2.3.2.2
119332lysine decarboxylase-4.1.1.18
119332ornithine decarboxylase-4.1.1.17
119332phenylalanine ammonia-lyase-4.3.1.24
119332tryptophan deaminase-
119332urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19996+-+-++-++++++-++++-
119332+++-+----++-+-+-+---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19996+--------+-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119332--+/----------------------+/----------------+/---+/-----+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11395soilYamanashiJapanJPNAsia
67770SoilYamanashi Pref.JapanJPNAsia
119332Environment, Woodland soilJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113951Risk group (German classification)
199961German classification
1193321Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Asanoa ferruginea gene for 16S ribosomal RNA, partial sequenceD869441468ena53367
20218C.ferruginea 16S rRNA geneX931991478ena53367
11395Asanoa ferruginea strain IMSNU 22009T 16S ribosomal RNA gene, partial sequenceAF1521081508ena53367

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Asanoa ferruginea NBRC 14496GCA_016862495contigncbi53367
66792Asanoa ferruginea strain DSM 4409953367.5wgspatric53367
66792Asanoa ferruginea strain NBRC 1449653367.6wgspatric53367
66792Asanoa ferruginea DSM 440992778260948draftimg53367
67770Asanoa ferruginea DSM 44099GCA_003387075contigncbi53367

GC content

@refGC-contentmethod
1139571.5
6777070.4-71.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes81no
gram-positiveyes88.825no
anaerobicno99.275no
halophileno93.636no
spore-formingyes93.024yes
glucose-utilyes90.036no
thermophileno98.545yes
motileno92.083no
flagellatedno98.379no
aerobicyes94.011no
glucose-fermentno91.051no

External links

@ref: 11395

culture collection no.: DSM 44099, ATCC 49966, IFO 14496, IMSNU 22009, JCM 7544, NBRC 14496, NRRL B-16430, CGMCC 4.1402, CIP 107013, HUT 6559, KCTC 9353, VKM Ac-1422

straininfo link

  • @ref: 77285
  • straininfo: 46135

literature

  • topic: Phylogeny
  • Pubmed-ID: 12054264
  • title: Proposal to transfer Catellatospora ferruginea and 'Catellatospora ishikariense' to Asanoa gen. nov. as Asanoa ferruginea comb. nov. and Asanoa ishikariensis sp. nov., with emended description of the genus Catellatospora.
  • authors: Lee SD, Hah YC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-3-967
  • year: 2002
  • mesh: Bacterial Typing Techniques, DNA, Ribosomal/analysis, Micromonosporaceae/chemistry/*classification/genetics/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11395Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44099)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44099
19996Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44099.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39635Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19207
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77285Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46135.1StrainInfo: A central database for resolving microbial strain identifiers
119332Curators of the CIPCollection of Institut Pasteur (CIP 107013)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107013