Strain identifier

BacDive ID: 7848

Type strain: Yes

Species: Asanoa ferruginea

Strain history: CIP <- 2001, JCM <- IFO <- I. Kawamoto: strain 6257-C, Kyowa Hakko Cie

NCBI tax ID(s): 53367 (species)

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General

@ref: 11395

BacDive-ID: 7848

DSM-Number: 44099

keywords: genome sequence, 16S sequence, Bacteria

description: Asanoa ferruginea DSM 44099 is a bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 53367
  • Matching level: species

strain history

@refhistory
11395<- IFO <- I. Kawamoto, Kyowa Hakko Co., 6257-C
67770IFO 14496 <-- I. Kawamoto 6257-C.
119332CIP <- 2001, JCM <- IFO <- I. Kawamoto: strain 6257-C, Kyowa Hakko Cie

doi: 10.13145/bacdive7848.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Asanoa
  • species: Asanoa ferruginea
  • full scientific name: Asanoa ferruginea (Asano and Kawamoto 1986) Lee and Hah 2002

  • synonyms

    • @ref: 20215
    • synonym: Catellatospora ferruginea

@ref: 11395

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Asanoa

species: Asanoa ferruginea

full scientific name: Asanoa ferruginea (Asano and Kawamoto 1986) Lee and Hah 2002

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
119332positiverod-shapedno
69480positive91.254

colony morphology

@refcolony colorincubation periodmedium used
19996Pure orange (2004)10-14 daysISP 2
19996Bright red orange (2008)10-14 daysISP 3
19996Colorless10-14 daysISP 4
19996Colorless10-14 daysISP 5
19996Pure orange (2004)10-14 daysISP 6
19996Pure orange (2004)10-14 daysISP 7

multicellular morphology

@refmedium nameforms multicellular complex
19996ISP 2
19996ISP 3no
19996ISP 4no
19996ISP 5no
19996ISP 6no
19996ISP 7no

multimedia

@refmultimedia contentintellectual property rightscaption
11395https://www.dsmz.de/microorganisms/photos/DSM_44099-1.jpg© Leibniz-Institut DSMZ
11395https://www.dsmz.de/microorganisms/photos/DSM_44099.jpg© Leibniz-Institut DSMZMedium 65 28°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11395GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19996ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19996ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19996ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19996ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19996ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19996ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
39635MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
11395N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
119332CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperature
19996positiveoptimum28
11395positivegrowth28
39635positivegrowth30
67770positivegrowth28
119332positivegrowth25-30
119332nogrowth10
119332nogrowth37
119332nogrowth41
119332nogrowth45

Physiology and metabolism

spore formation

  • @ref: 19996
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
19996NaClpositivegrowth0 %
119332NaClpositivegrowth0 %
119332NaClnogrowth2 %
119332NaClnogrowth4 %
119332NaClnogrowth6 %
119332NaClnogrowth8 %
119332NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-10(H8), MK-10(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1999617234glucose+/-
1999622599arabinose+/-
1999617992sucrose+/-
1999618222xylose+/-
1999617268myo-inositol+/-
1999629864mannitol+/-
1999628757fructose+/-
1999626546rhamnose+/-
1999616634raffinose+/-
1999662968cellulose+/-
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
11933216947citrate-carbon source
1193324853esculin+hydrolysis
119332606565hippurate+hydrolysis
11933217632nitrate-reduction
11933216301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11933235581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11933215688acetoin-
11933217234glucose-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
119332oxidase-
119332beta-galactosidase+3.2.1.23
119332alcohol dehydrogenase-1.1.1.1
119332gelatinase-
119332catalase+1.11.1.6
119332gamma-glutamyltransferase+2.3.2.2
119332lysine decarboxylase-4.1.1.18
119332ornithine decarboxylase-4.1.1.17
119332phenylalanine ammonia-lyase-4.3.1.24
119332tryptophan deaminase-
119332urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19996+-+-++-++++++-++++-
119332+++-+----++-+-+-+---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19996+--------+-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119332--+/----------------------+/----------------+/---+/-----+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11395soilYamanashiJapanJPNAsia
67770SoilYamanashi Pref.JapanJPNAsia
119332Environment, Woodland soilJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113951Risk group (German classification)
199961German classification
1193321Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Asanoa ferruginea gene for 16S ribosomal RNA, partial sequenceD869441468nuccore53367
20218C.ferruginea 16S rRNA geneX931991478nuccore53367
11395Asanoa ferruginea strain IMSNU 22009T 16S ribosomal RNA gene, partial sequenceAF1521081508nuccore53367

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Asanoa ferruginea NBRC 14496GCA_016862495contigncbi53367
66792Asanoa ferruginea strain DSM 4409953367.5wgspatric53367
66792Asanoa ferruginea strain NBRC 1449653367.6wgspatric53367
66792Asanoa ferruginea DSM 440992778260948draftimg53367
67770Asanoa ferruginea DSM 44099GCA_003387075contigncbi53367

GC content

@refGC-contentmethod
1139571.5
6777070.4-71.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes81no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes91.254no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no93.947no
69480spore-formingspore-formingAbility to form endo- or exosporesyes89.551no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes88.962no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97no
69480flagellatedmotile2+Ability to perform flagellated movementno88no

External links

@ref: 11395

culture collection no.: DSM 44099, ATCC 49966, IFO 14496, IMSNU 22009, JCM 7544, NBRC 14496, NRRL B-16430, CGMCC 4.1402, CIP 107013, HUT 6559, KCTC 9353, VKM Ac-1422

straininfo link

  • @ref: 77285
  • straininfo: 46135

literature

  • topic: Phylogeny
  • Pubmed-ID: 12054264
  • title: Proposal to transfer Catellatospora ferruginea and 'Catellatospora ishikariense' to Asanoa gen. nov. as Asanoa ferruginea comb. nov. and Asanoa ishikariensis sp. nov., with emended description of the genus Catellatospora.
  • authors: Lee SD, Hah YC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-3-967
  • year: 2002
  • mesh: Bacterial Typing Techniques, DNA, Ribosomal/analysis, Micromonosporaceae/chemistry/*classification/genetics/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11395Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44099)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44099
19996Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44099.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39635Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19207
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77285Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46135.1StrainInfo: A central database for resolving microbial strain identifiers
119332Curators of the CIPCollection of Institut Pasteur (CIP 107013)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107013