Strain identifier

BacDive ID: 7842

Type strain: Yes

Species: Actinoplanes sichuanensis

Strain Designation: 03-723

Strain history: <- YQ Zhang, IMB, CAMS & Peking Union Medical College, China

NCBI tax ID(s): 512349 (species)

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General

@ref: 15645

BacDive-ID: 7842

DSM-Number: 45183

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile

description: Actinoplanes sichuanensis 03-723 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 512349
  • Matching level: species

strain history

@refhistory
15645<- Y.-Q. Zhang; 03-723 <- Y.-Z. Wei and et al.
67770CCM 7526 <-- W. Sun et al. 03-723.
67771<- YQ Zhang, IMB, CAMS & Peking Union Medical College, China

doi: 10.13145/bacdive7842.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinoplanes
  • species: Actinoplanes sichuanensis
  • full scientific name: Actinoplanes sichuanensis Sun et al. 2009

@ref: 15645

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinoplanes

species: Actinoplanes sichuanensis

full scientific name: Actinoplanes sichuanensis Sun et al. 2009

strain designation: 03-723

type strain: yes

Morphology

cell morphology

@refgram stainmotility
29303positiveyes
67771positive

colony morphology

@refcolony colorincubation periodmedium used
19921Pastel yellow10-14 daysISP 2
19921Pastel yellow10-14 daysISP 3
19921Sand yellow10-14 daysISP 4
19921Chrome yellow10-14 daysISP 5
19921Grey yellow10-14 daysISP 6
19921Pastell yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19921noISP 2
19921noISP 3
19921noISP 4
19921noISP 5
19921noISP 6
19921noISP 7

pigmentation

  • @ref: 29303
  • production: yes

multimedia

  • @ref: 15645
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45183.jpg
  • caption: Medium 553 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15645GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
19921ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19921ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19921ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19921ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19921ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19921ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
15645TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
15645BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
15645GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15645positivegrowth28mesophilic
19921positiveoptimum28mesophilic
29303positivegrowth10-37
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

  • @ref: 29303
  • ability: positive
  • type: growth
  • pH: 6.5-10.5
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29303aerobe
67771aerobe

spore formation

  • @ref: 29303
  • spore formation: yes

halophily

  • @ref: 29303
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-4 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1992117234glucose+
1992122599arabinose+
1992117992sucrose+/-
1992118222xylose+/-
1992117268myo-inositol+
1992129864mannitol+
1992128757fructose+
1992126546rhamnose+/-
1992116634raffinose+/-
1992162968cellulose+/-
2930330089acetate+carbon source
2930315963ribitol+carbon source
2930322599arabinose+carbon source
2930316947citrate+carbon source
2930328757fructose+carbon source
2930328260galactose+carbon source
2930324265gluconate+carbon source
293035417glucosamine+carbon source
2930317234glucose+carbon source
2930315792malonate+carbon source
2930317306maltose+carbon source
2930333942ribose+carbon source
2930330911sorbitol+carbon source
2930317992sucrose+carbon source
2930318222xylose+carbon source
293034853esculin+hydrolysis
2930317632nitrate+reduction

enzymes

@refvalueactivityec
29303gelatinase+
29303urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19921+++-++---++-+-++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15645soilSichuan ProvinceChinaCHNAsia
67770SoilSichuan Province, south-west ChinaChinaCHNAsia
67771From soilSichuan ProvinceChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_27074.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_240;97_16361;98_20282;99_27074&stattab=map
  • Last taxonomy: Actinoplanes sichuanensis
  • 16S sequence: EU531458
  • Sequence Identity:
  • Total samples: 35
  • soil counts: 11
  • aquatic counts: 14
  • animal counts: 9
  • plant counts: 1

Safety information

risk assessment

  • @ref: 15645
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15645
  • description: Actinoplanes sichuanensis strain 03-723 16S ribosomal RNA gene, partial sequence
  • accession: EU531458
  • length: 1438
  • database: ena
  • NCBI tax ID: 512349

GC content

@refGC-contentmethod
1564570.4thermal denaturation, midpoint method (Tm)
2930370.4

External links

@ref: 15645

culture collection no.: DSM 45183, CCM 7526, KCTC 19460, JCM 16605

straininfo link

  • @ref: 77279
  • straininfo: 399735

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19625414Actinoplanes sichuanensis sp. nov. and Actinoplanes xinjiangensis sp. nov.Sun W, Dong GX, Zhang YQ, Wei YZ, Li QP, Yu LY, Klenk HP, Zhang YQInt J Syst Evol Microbiol10.1099/ijs.0.010561-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Micromonosporaceae/*classification/genetics/*isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyGenetics
Phylogeny23114571Actinoplanes hulinensis sp. nov., a novel actinomycete isolated from soybean root (Glycine max (L.) Merr).Shen Y, Liu C, Wang X, Zhao J, Jia F, Zhang Y, Wang L, Yang D, Xiang WAntonie Van Leeuwenhoek10.1007/s10482-012-9809-92012Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Micromonosporaceae/*classification/genetics/*isolation & purification/physiology, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soybeans/*microbiologyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15645Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45183)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45183
19921Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45183.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29303Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2571728776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77279Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID399735.1StrainInfo: A central database for resolving microbial strain identifiers