Strain identifier

BacDive ID: 7811

Type strain: Yes

Species: Actinoplanes rectilineatus

Strain Designation: LL 7-10 (IMRU 3919), MB-T 24

Strain history: KCC A-0194 <-- H. A. Lechevalier 7-10 (IMRU 3919).

NCBI tax ID(s): 113571 (species)

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General

@ref: 11282

BacDive-ID: 7811

DSM-Number: 43808

keywords: genome sequence, 16S sequence, Bacteria, spore-forming

description: Actinoplanes rectilineatus LL 7-10 is a spore-forming bacterium that was isolated from garden soil.

NCBI tax id

  • NCBI tax id: 113571
  • Matching level: species

strain history

@refhistory
11282<- G. Vobis, MB-T 24 <- H.A. Lechevalier, LL 7-10, IMRU
67770KCC A-0194 <-- H. A. Lechevalier 7-10 (IMRU 3919).

doi: 10.13145/bacdive7811.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinoplanes
  • species: Actinoplanes rectilineatus
  • full scientific name: Actinoplanes rectilineatus Lechevalier and Lechevalier 1975 (Approved Lists 1980)

@ref: 11282

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinoplanes

species: Actinoplanes rectilineatus

full scientific name: Actinoplanes rectilineatus Lechevalier and Lechevalier 1975 emend. Nouioui et al. 2018

strain designation: LL 7-10 (IMRU 3919), MB-T 24

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19593Yellow orange10-14 daysISP 2
19593Brown orange10-14 daysISP 3
19593Brown orange10-14 daysISP 4
19593Brown orange10-14 daysISP 5
19593Brown orange10-14 daysISP 6
19593Brown orange10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19593noISP 2
19593noISP 3
19593noISP 4
19593noISP 5
19593noISP 6
19593noISP 7

multimedia

  • @ref: 11282
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43808.jpg
  • caption: Medium 83 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11282CZAPEK PEPTONE AGAR (DSMZ Medium 83)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium83.pdf
19593ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19593ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19593ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19593ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19593ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19593ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11282GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
19593positiveoptimum28
11282positivegrowth28
67770positivegrowth28

Physiology and metabolism

spore formation

  • @ref: 19593
  • spore description: Formation of sporangia, spore surface smooth
  • type of spore: spore
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19593+++-+-++++++++++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19593+++++-+--++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11282garden soilNew Jersey, SommersetUSAUSANorth America
67770Garden soilSomerset, NJUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Engineered#Agriculture#Garden

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
112821Risk group (German classification)
195931Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Actinoplanes rectilineatus gene for 16S rRNA, complete sequence
  • accession: AB037010
  • length: 1473
  • database: nuccore
  • NCBI tax ID: 113571

Genome sequences

  • @ref: 67770
  • description: Actinoplanes rectilineatus NRRL B-16090
  • accession: GCA_000962825
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 113571

GC content

  • @ref: 67770
  • GC-content: 70.6
  • method: genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes87.583no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no93.784no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes86.169no
69480spore-formingspore-formingAbility to form endo- or exosporesyes87.075yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.25yes
69480flagellatedmotile2+Ability to perform flagellated movementno59.6no

External links

@ref: 11282

culture collection no.: DSM 43808, ATCC 29234, BCC 60402, IFO 13941, IMRU 3919, KCC A-0194, NBRC 13941, TBRC 2207, JCM 3194, BCRC 13338, IFM 1260, IMET 9276, IMSNU 20041, KCTC 9540, NCIMB 12746, NRRL B-16090

straininfo link

  • @ref: 77253
  • straininfo: 39422

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26311536Actinoplanes luteus sp. nov., isolated from soil.Suriyachadkun C, Ngaemthao W, Chunhametha S, Sanglier JJInt J Syst Evol Microbiol10.1099/ijsem.0.0005622015Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Forests, Micromonosporaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
31912451Development of a gene expression system for the uncommon actinomycete Actinoplanes rectilineatus NRRL B-16090.Yushchuk O, Homoniuk V, Datsiuk Y, Ostash B, Marinelli F, Fedorenko VJ Appl Genet10.1007/s13353-019-00534-72020Actinoplanes/*genetics/ultrastructure, *Gene Expression, Gene Expression Regulation, Genetic Engineering, Genetic Vectors/genetics, Plasmids/*genetics, Promoter Regions, Genetic, Spores, Bacterial/ultrastructure
Phylogeny34324105Actinoplanes aureus sp. nov., a novel protease-producing actinobacterium isolated from soil.Song J, Sun X, Luo X, He C, Huang Z, Zhao J, He B, Du X, Wang X, Xiang WAntonie Van Leeuwenhoek10.1007/s10482-021-01617-42021*Actinoplanes/classification/isolation & purification, Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptide Hydrolases/metabolism, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivativesTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11282Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43808)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43808
19593Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43808.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77253Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39422.1StrainInfo: A central database for resolving microbial strain identifiers