Strain identifier

BacDive ID: 7811

Type strain: Yes

Species: Actinoplanes rectilineatus

Strain Designation: LL 7-10 (IMRU 3919), MB-T 24

Strain history: KCC A-0194 <-- H. A. Lechevalier 7-10 (IMRU 3919).

NCBI tax ID(s): 113571 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 11282

BacDive-ID: 7811

DSM-Number: 43808

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Actinoplanes rectilineatus LL 7-10 is a spore-forming, mesophilic bacterium that was isolated from garden soil.

NCBI tax id

  • NCBI tax id: 113571
  • Matching level: species

strain history

@refhistory
11282<- G. Vobis, MB-T 24 <- H.A. Lechevalier, LL 7-10, IMRU
67770KCC A-0194 <-- H. A. Lechevalier 7-10 (IMRU 3919).

doi: 10.13145/bacdive7811.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinoplanes
  • species: Actinoplanes rectilineatus
  • full scientific name: Actinoplanes rectilineatus Lechevalier and Lechevalier 1975 (Approved Lists 1980)

@ref: 11282

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinoplanes

species: Actinoplanes rectilineatus

full scientific name: Actinoplanes rectilineatus Lechevalier and Lechevalier 1975 emend. Nouioui et al. 2018

strain designation: LL 7-10 (IMRU 3919), MB-T 24

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19593Yellow orange10-14 daysISP 2
19593Brown orange10-14 daysISP 3
19593Brown orange10-14 daysISP 4
19593Brown orange10-14 daysISP 5
19593Brown orange10-14 daysISP 6
19593Brown orange10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19593noISP 2
19593noISP 3
19593noISP 4
19593noISP 5
19593noISP 6
19593noISP 7

multimedia

  • @ref: 11282
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43808.jpg
  • caption: Medium 83 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11282CZAPEK PEPTONE AGAR (DSMZ Medium 83)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium83.pdf
19593ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19593ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19593ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19593ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19593ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19593ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11282GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19593positiveoptimum28mesophilic
11282positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19593
  • spore description: Formation of sporangia, spore surface smooth
  • type of spore: spore
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19593+++-+-++++++++++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19593+++++-+--++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11282garden soilNew Jersey, SommersetUSAUSANorth America
67770Garden soilSomerset, NJUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Engineered#Agriculture#Garden

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
112821Risk group (German classification)
195931Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Actinoplanes rectilineatus gene for 16S rRNA, complete sequence
  • accession: AB037010
  • length: 1473
  • database: ena
  • NCBI tax ID: 113571

Genome sequences

  • @ref: 67770
  • description: Actinoplanes rectilineatus NRRL B-16090
  • accession: GCA_000962825
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 113571

GC content

  • @ref: 67770
  • GC-content: 70.6
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes69.654no
flagellatedno86.209no
gram-positiveyes88.247no
anaerobicno99.239no
aerobicyes93.555no
halophileno94.896no
spore-formingyes92.797yes
glucose-utilyes89.146no
thermophileno99.249yes
glucose-fermentno87.318no

External links

@ref: 11282

culture collection no.: DSM 43808, ATCC 29234, BCC 60402, IFO 13941, IMRU 3919, KCC A-0194, NBRC 13941, TBRC 2207, JCM 3194, BCRC 13338, IFM 1260, IMET 9276, IMSNU 20041, KCTC 9540, NCIMB 12746, NRRL B-16090

straininfo link

  • @ref: 77253
  • straininfo: 39422

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26311536Actinoplanes luteus sp. nov., isolated from soil.Suriyachadkun C, Ngaemthao W, Chunhametha S, Sanglier JJInt J Syst Evol Microbiol10.1099/ijsem.0.0005622015Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Forests, Micromonosporaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
31912451Development of a gene expression system for the uncommon actinomycete Actinoplanes rectilineatus NRRL B-16090.Yushchuk O, Homoniuk V, Datsiuk Y, Ostash B, Marinelli F, Fedorenko VJ Appl Genet10.1007/s13353-019-00534-72020Actinoplanes/*genetics/ultrastructure, *Gene Expression, Gene Expression Regulation, Genetic Engineering, Genetic Vectors/genetics, Plasmids/*genetics, Promoter Regions, Genetic, Spores, Bacterial/ultrastructure
Phylogeny34324105Actinoplanes aureus sp. nov., a novel protease-producing actinobacterium isolated from soil.Song J, Sun X, Luo X, He C, Huang Z, Zhao J, He B, Du X, Wang X, Xiang WAntonie Van Leeuwenhoek10.1007/s10482-021-01617-42021*Actinoplanes/classification/isolation & purification, Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptide Hydrolases/metabolism, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivativesTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11282Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43808)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43808
19593Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43808.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
77253Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39422.1StrainInfo: A central database for resolving microbial strain identifiers