Strain identifier

BacDive ID: 7581

Type strain: Yes

Species: Pseudarthrobacter chlorophenolicus

Strain Designation: A6

Strain history: KCTC 9906 <-- DSM 12829 <-- J. K. Jansson A6.

NCBI tax ID(s): 85085 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4844

BacDive-ID: 7581

DSM-Number: 12829

keywords: mesophilic, Bacteria, 16S sequence, genome sequence

description: Pseudarthrobacter chlorophenolicus A6 is a mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 85085
  • Matching level: species

strain history

doi: 10.13145/bacdive7581.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Pseudarthrobacter
  • species: Pseudarthrobacter chlorophenolicus
  • full scientific name: Pseudarthrobacter chlorophenolicus (Westerberg et al. 2000) Busse 2016
  • synonyms

    • @ref: 20215
    • synonym: Arthrobacter chlorophenolicus

@ref: 4844

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Pseudarthrobacter

species: Pseudarthrobacter chlorophenolicus

full scientific name: Pseudarthrobacter chlorophenolicus (Westerberg et al. 2000) Busse 2016

strain designation: A6

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18371Light ivory (1015)10-14 daysISP 2
18371Light ivory (1015)10-14 daysISP 3
18371Light ivory (1015)10-14 daysISP 4
18371Colorless10-14 daysISP 5
18371Colorless10-14 daysISP 6
18371Colorless10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18371noISP 2
18371noISP 3
18371noISP 4
18371noISP 5
18371noISP 6
18371noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4844TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://bacmedia.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18371ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18371ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18371ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18371ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18371ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18371ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
41906MEDIUM 368 - for Luria bertaniyesDistilled water make up to (1000.000 ml);Sodium chloride (10.000 g);Agar(15.000 g);Yeast extract (5.000 g);Tryptone (10.000 g)

culture temp

@refgrowthtypetemperaturerange
18371positiveoptimum28mesophilic
4844positivegrowth28mesophilic
41906positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

murein

  • @ref: 4844
  • murein short key: A11.23
  • type: A3alpha L-Lys-L-Ser-L-Thr-L-Ala

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837928087glycogen-fermentation
6837917992sucrose-fermentation
6837917716lactose-fermentation
6837917306maltose-fermentation
6837916899D-mannitol-fermentation
6837965327D-xylose-fermentation
6837916988D-ribose-fermentation
6837917634D-glucose-fermentation
683795291gelatin+hydrolysis
6837916199urea+hydrolysis
683794853esculin+hydrolysis
6837917632nitrate-reduction

enzymes

@refvalueactivityec
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18371----+++-+++--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18371--+-+--+-+-++-++-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4844soilUSA, Colorado, Fort CollinsUSAUSANorth America
67770SoilFort Collins, COUSAUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
183711
48441Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4844
  • description: Arthrobacter chlorophenolicus 16S ribosomal RNA gene, partial sequence
  • accession: AF102267
  • length: 1446
  • database: ena
  • NCBI tax ID: 85085

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudarthrobacter chlorophenolicus A6 A6GCA_000022025completencbi452863
66792Pseudarthrobacter chlorophenolicus A6GCA_900104315contigncbi85085
66792Arthrobacter chlorophenolicus A6452863.6completepatric452863
66792Pseudarthrobacter chlorophenolicus A6452863.41plasmidpatric452863
66792Pseudarthrobacter chlorophenolicus A6452863.40plasmidpatric452863
66792Pseudarthrobacter chlorophenolicus strain A685085.8wgspatric85085
66792Pseudarthrobacter chlorophenolicus A6643348509completeimg452863
66792Pseudarthrobacter chlorophenolicus DSM 128292634166551draftimg452863
67770Pseudarthrobacter chlorophenolicus A6 chromosome, complete genomeCP001341ena452863
67770Pseudarthrobacter chlorophenolicus strain A6, whole genome shotgun sequencing projectFNLE00000000ncbi85085

GC content

  • @ref: 4844
  • GC-content: 65.1
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 4844

culture collection no.: DSM 12829, ATCC 700700, CIP 107037, JCM 12360, KCTC 9906, NCIMB 13794, MTCC 3706

straininfo link

@refpassport
20218http://www.straininfo.net/strains/118363
20218http://www.straininfo.net/strains/118361
20218http://www.straininfo.net/strains/404022
20218http://www.straininfo.net/strains/556189
20218http://www.straininfo.net/strains/321987
20218http://www.straininfo.net/strains/404021

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11155983Arthrobacter chlorophenolicus sp. nov., a new species capable of degrading high concentrations of 4-chlorophenol.Westerberg K, Elvang AM, Stackebrandt E, Jansson JKInt J Syst Evol Microbiol10.1099/00207713-50-6-20832000Arthrobacter/chemistry/*classification/genetics/growth & development/metabolism, Bacterial Typing Techniques, Biodegradation, Environmental, Chlorophenols/*metabolism, Culture Media, DNA, Ribosomal/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenols/metabolism, Phylogeny, Pigments, Biological/biosynthesis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants/metabolismMetabolism
Phylogeny18676480Arthrobacter defluvii sp. nov., 4-chlorophenol-degrading bacteria isolated from sewage.Kim KK, Lee KC, Oh HM, Kim MJ, Eom MK, Lee JSInt J Syst Evol Microbiol10.1099/ijs.0.65550-02008Arthrobacter/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Chlorophenols/*metabolism, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Species SpecificityGenetics
Phylogeny25052022Arthrobacter enclensis sp. nov., isolated from sediment sample.Dastager SG, Liu Q, Tang SK, Krishnamurthi S, Lee JC, Li WJArch Microbiol10.1007/s00203-014-1016-92014Arthrobacter/chemistry/*classification/genetics, Base Composition, Fatty Acids/analysis, Geologic Sediments/*microbiology, India, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4844Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12829)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12829
18371Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM12829.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41906Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19234
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym