Strain identifier

BacDive ID: 7580

Type strain: Yes

Species: Arthrobacter woluwensis

Strain history: CIP <- 2013, DSMZ <- 1996, G. Funke, Inst. Med. Mikrobiol., Zurich, Switzerland: strain DMMZ 1551 <- G. Wauters: CUL 1808

NCBI tax ID(s): 1216974 (strain), 156980 (species)

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General

@ref: 3942

BacDive-ID: 7580

DSM-Number: 10495

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-positive

description: Arthrobacter woluwensis CCUG 36790 is an obligate aerobe, Gram-positive bacterium that was isolated from blood culture.

NCBI tax id

NCBI tax idMatching level
1216974strain
156980species

strain history

@refhistory
3942<- G. Funke, CUL 1808
67770CCUG 36790 <-- G. Funke DMMZ 1551 <-- G. Wauters CUL 1808.
123957CIP <- 2013, DSMZ <- 1996, G. Funke, Inst. Med. Mikrobiol., Zurich, Switzerland: strain DMMZ 1551 <- G. Wauters: CUL 1808

doi: 10.13145/bacdive7580.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter woluwensis
  • full scientific name: Arthrobacter woluwensis Funke et al. 1997

@ref: 3942

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter woluwensis

full scientific name: Arthrobacter woluwensis Funke et al. 1997

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
123957positiverod-shapedno
69480positive92.758
69480no94

colony morphology

@refcolony colorincubation periodmedium usedhemolysis ability
18354Light ivory (1015)10-14 daysISP 2
18354Light ivory (1015)10-14 daysISP 3
18354Light ivory (1015)10-14 daysISP 4
18354Light ivory (1015)10-14 daysISP 5
18354Light ivory (1015)10-14 daysISP 6
18354Light ivory (1015)10-14 daysISP 7
1239571

multicellular morphology

@refforms multicellular complexmedium name
18354noISP 2
18354noISP 3
18354noISP 4
18354noISP 5
18354noISP 6
18354noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3942TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18354ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18354ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18354ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18354ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18354ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18354ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
37305MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123957CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
18354positiveoptimum28
3942positivegrowth30
37305positivegrowth37
67770positivegrowth37
123957positivegrowth15-41
123957nogrowth10
123957nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 123957
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
123957NaClpositivegrowth0-6 %
123957NaClnogrowth8 %
123957NaClnogrowth10 %

murein

  • @ref: 3942
  • murein short key: A11.60
  • type: A4alpha L-Lys-D-Asp; alpha-carboxyl group of D-Glu substituted by glycine

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12395716947citrate+carbon source
1239574853esculin+hydrolysis
123957606565hippurate+hydrolysis
12395717632nitrate-reduction
12395716301nitrite-reduction
12395717632nitrate-respiration
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 123957
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123957
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12395715688acetoin-
12395717234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382acid phosphatase+3.1.3.2
68382trypsin+3.4.21.4
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
123957oxidase-
123957beta-galactosidase+3.2.1.23
123957alcohol dehydrogenase-1.1.1.1
123957gelatinase+
123957amylase-
123957DNase-
123957caseinase-3.4.21.50
123957catalase-1.11.1.6
123957tween esterase-
123957gamma-glutamyltransferase+2.3.2.2
123957lecithinase+
123957lipase-
123957lysine decarboxylase-4.1.1.18
123957ornithine decarboxylase-4.1.1.17
123957phenylalanine ammonia-lyase-4.3.1.24
123957tryptophan deaminase-
123957urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    53394C14:0114
    53394C15:00.215
    53394C16:07.416
    53394C18:01.618
    53394C14:0 ISO2.713.618
    53394C15:0 ANTEISO43.214.711
    53394C15:0 ISO18.714.621
    53394C16:0 iso9.415.626
    53394C17:0 anteiso10.416.722
    53394C17:0 iso2.716.629
    53394C18:1 2OH0.219.088
    53394C18:1 ω9c1.417.769
    53394C18:2 ω6,9c/C18:0 ANTE1.217.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18354-++--++++++--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18354+-++++++++-++-++++-
123957++++++-+-++-+-++++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123957+++++-+++++++++++++---+--++--+-+-++++----+-------+++-+--+--++++++-+----+++---++++-----+--++++++-+++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
3942blood cultureBelgiumBELEurope
53394Human blood cultureBelgiumBELEuropeLouvain
67770Blood culture
123957Human, BloodBelgiumBELEuropeBrussels

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Blood culture
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_2506.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1351;97_1604;98_1950;99_2506&stattab=map
  • Last taxonomy: Arthrobacter
  • 16S sequence: X93353
  • Sequence Identity:
  • Total samples: 7625
  • soil counts: 2930
  • aquatic counts: 827
  • animal counts: 3190
  • plant counts: 678

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
183542
39422Risk group (German classification)
1239571Risk group (French classification)

Sequence information

16S sequences

  • @ref: 3942
  • description: Arthrobacter woluwensis 16S rRNA gene (strain 1551)
  • accession: X93353
  • length: 1477
  • database: nuccore
  • NCBI tax ID: 156980

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arthrobacter woluwensis NBRC 1078401216974.3wgspatric1216974
66792Arthrobacter woluwensis strain DSM 10495156980.3wgspatric156980
66792Arthrobacter woluwensis DSM 104952639762519draftimg156980
66792Arthrobacter woluwensis NBRC 1078402731957570draftimg1216974
67770Arthrobacter woluwensis NBRC 107840GCA_001552095contigncbi1216974
67770Arthrobacter woluwensis DSM 10495GCA_900105345contigncbi156980

GC content

  • @ref: 3942
  • GC-content: 69
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes92.758no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.551no
69480spore-formingspore-formingAbility to form endo- or exosporesno69.213no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes87.859no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.467yes
69480flagellatedmotile2+Ability to perform flagellated movementno94no

External links

@ref: 3942

culture collection no.: CCUG 36790, JCM 11679, DSM 10495, CIP 104908, CUL 1808, DMMZ 1551, ATCC 700220, CCM 4575, IFM 10486, KCTC 9905, NBRC 107840

straininfo link

  • @ref: 77024
  • straininfo: 45411

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3942Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10495)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10495
18354Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM10495.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37305Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16869
53394Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 36790)https://www.ccug.se/strain?id=36790
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77024Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45411.1StrainInfo: A central database for resolving microbial strain identifiers
123957Curators of the CIPCollection of Institut Pasteur (CIP 104908)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104908