Strain identifier

BacDive ID: 7580

Type strain: Yes

Species: Arthrobacter woluwensis

Strain history: CIP <- 2013, DSMZ <- 1996, G. Funke, Inst. Med. Mikrobiol., Zurich, Switzerland: strain DMMZ 1551 <- G. Wauters: CUL 1808

NCBI tax ID(s): 1216974 (strain), 156980 (species)

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General

@ref: 3942

BacDive-ID: 7580

DSM-Number: 10495

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Arthrobacter woluwensis CCUG 36790 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from blood culture.

NCBI tax id

NCBI tax idMatching level
156980species
1216974strain

strain history

@refhistory
3942<- G. Funke, CUL 1808
67770CCUG 36790 <-- G. Funke DMMZ 1551 <-- G. Wauters CUL 1808.
123957CIP <- 2013, DSMZ <- 1996, G. Funke, Inst. Med. Mikrobiol., Zurich, Switzerland: strain DMMZ 1551 <- G. Wauters: CUL 1808

doi: 10.13145/bacdive7580.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter woluwensis
  • full scientific name: Arthrobacter woluwensis Funke et al. 1997

@ref: 3942

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter woluwensis

full scientific name: Arthrobacter woluwensis Funke et al. 1997

type strain: yes

Morphology

cell morphology

  • @ref: 123957
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium usedhemolysis ability
18354Light ivory (1015)10-14 daysISP 2
18354Light ivory (1015)10-14 daysISP 3
18354Light ivory (1015)10-14 daysISP 4
18354Light ivory (1015)10-14 daysISP 5
18354Light ivory (1015)10-14 daysISP 6
18354Light ivory (1015)10-14 daysISP 7
1239571

multicellular morphology

@refforms multicellular complexmedium name
18354noISP 2
18354noISP 3
18354noISP 4
18354noISP 5
18354noISP 6
18354noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3942TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18354ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18354ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18354ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18354ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18354ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18354ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
37305MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123957CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
18354positiveoptimum28mesophilic
3942positivegrowth30mesophilic
37305positivegrowth37mesophilic
67770positivegrowth37mesophilic
123957positivegrowth15-41
123957nogrowth10psychrophilic
123957nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123957
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
123957NaClpositivegrowth0-6 %
123957NaClnogrowth8 %
123957NaClnogrowth10 %

murein

  • @ref: 3942
  • murein short key: A11.60
  • type: A4alpha L-Lys-D-Asp; alpha-carboxyl group of D-Glu substituted by glycine

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12395716947citrate+carbon source
1239574853esculin+hydrolysis
123957606565hippurate+hydrolysis
12395717632nitrate-reduction
12395716301nitrite-reduction
12395717632nitrate-respiration
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 123957
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123957
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12395715688acetoin-
12395717234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382acid phosphatase+3.1.3.2
68382trypsin+3.4.21.4
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
123957oxidase-
123957beta-galactosidase+3.2.1.23
123957alcohol dehydrogenase-1.1.1.1
123957gelatinase+
123957amylase-
123957DNase-
123957caseinase-3.4.21.50
123957catalase-1.11.1.6
123957tween esterase-
123957gamma-glutamyltransferase+2.3.2.2
123957lecithinase+
123957lipase-
123957lysine decarboxylase-4.1.1.18
123957ornithine decarboxylase-4.1.1.17
123957phenylalanine ammonia-lyase-4.3.1.24
123957tryptophan deaminase-
123957urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    53394C14:0114
    53394C15:00.215
    53394C16:07.416
    53394C18:01.618
    53394C14:0 ISO2.713.618
    53394C15:0 ANTEISO43.214.711
    53394C15:0 ISO18.714.621
    53394C16:0 iso9.415.626
    53394C17:0 anteiso10.416.722
    53394C17:0 iso2.716.629
    53394C18:1 2OH0.219.088
    53394C18:1 ω9c1.417.769
    53394C18:2 ω6,9c/C18:0 ANTE1.217.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18354-++--++++++--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18354+-++++++++-++-++++-
123957++++++-+-++-+-++++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123957+++++-+++++++++++++---+--++--+-+-++++----+-------+++-+--+--++++++-+----+++---++++-----+--++++++-+++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
3942blood cultureBelgiumBELEurope
53394Human blood cultureBelgiumBELEuropeLouvain
67770Blood culture
123957Human, BloodBelgiumBELEuropeBrussels

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Blood culture
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_2506.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1351;97_1604;98_1950;99_2506&stattab=map
  • Last taxonomy: Arthrobacter
  • 16S sequence: X93353
  • Sequence Identity:
  • Total samples: 7625
  • soil counts: 2930
  • aquatic counts: 827
  • animal counts: 3190
  • plant counts: 678

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
183542
39422Risk group (German classification)
1239571Risk group (French classification)

Sequence information

16S sequences

  • @ref: 3942
  • description: Arthrobacter woluwensis 16S rRNA gene (strain 1551)
  • accession: X93353
  • length: 1477
  • database: ena
  • NCBI tax ID: 156980

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arthrobacter woluwensis NBRC 1078401216974.3wgspatric1216974
66792Arthrobacter woluwensis strain DSM 10495156980.3wgspatric156980
66792Arthrobacter woluwensis DSM 104952639762519draftimg156980
66792Arthrobacter woluwensis NBRC 1078402731957570draftimg1216974
67770Arthrobacter woluwensis NBRC 107840GCA_001552095contigncbi1216974
67770Arthrobacter woluwensis DSM 10495GCA_900105345contigncbi156980

GC content

  • @ref: 3942
  • GC-content: 69
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno92.21no
gram-positiveyes93.718no
anaerobicno99.571no
aerobicyes95.63no
halophileno88.908no
spore-formingno92.07no
thermophileno99.008yes
glucose-utilyes88.674no
flagellatedno96.822no
glucose-fermentno89.781yes

External links

@ref: 3942

culture collection no.: CCUG 36790, JCM 11679, DSM 10495, CIP 104908, CUL 1808, DMMZ 1551, ATCC 700220, CCM 4575, IFM 10486, KCTC 9905, NBRC 107840

straininfo link

  • @ref: 77024
  • straininfo: 45411

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3942Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10495)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10495
18354Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM10495.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37305Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16869
53394Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 36790)https://www.ccug.se/strain?id=36790
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77024Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45411.1StrainInfo: A central database for resolving microbial strain identifiers
123957Curators of the CIPCollection of Institut Pasteur (CIP 104908)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104908