Strain identifier

BacDive ID: 7378

Type strain: No

Species: Microbacterium schleiferi

Strain Designation: Co 112, CO112

Strain history: CIP <- 1987, DSM <- K.H. Schleifer <- H. Seiler: strain CO112

NCBI tax ID(s): 69362 (species)

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General

@ref: 8954

BacDive-ID: 7378

DSM-Number: 20606

keywords: Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Microbacterium schleiferi Co 112 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from activated sludge.

NCBI tax id

  • NCBI tax id: 69362
  • Matching level: species

strain history

@refhistory
8954<- K.H. Schleifer <- H. Seiler, Co 112 (Corynebacterium alcanolyticum)
122509CIP <- 1987, DSM <- K.H. Schleifer <- H. Seiler: strain CO112

doi: 10.13145/bacdive7378.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Microbacterium
  • species: Microbacterium schleiferi
  • full scientific name: Microbacterium schleiferi (Yokota et al. 1993) Takeuchi and Hatano 1998

  • synonyms

    • @ref: 20215
    • synonym: Aureobacterium schleiferi

@ref: 8954

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Microbacterium

species: Microbacterium schleiferi

full scientific name: Microbacterium schleiferi (Yokota et al. 1993) Takeuchi and Hatano 1998

strain designation: Co 112, CO112

type strain: no

Morphology

cell morphology

  • @ref: 122509
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 122509

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8954CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://mediadive.dsmz.de/medium/53Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
40472MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
122509CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
8954positivegrowth30
40472positivegrowth30
122509positivegrowth22-37
122509nogrowth10
122509nogrowth15
122509nogrowth41
122509nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 122509
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
122509NaClpositivegrowth0-6 %
122509NaClnogrowth8 %
122509NaClnogrowth10 %

murein

  • @ref: 8954
  • murein short key: B06
  • type: B2ß {Gly} [L-Hsr] D-Glu(Hyg)-Gly-D-Orn

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12250916947citrate-carbon source
1225094853esculin+hydrolysis
122509606565hippurate-hydrolysis
12250917632nitrate-reduction
12250916301nitrite-reduction
12250917632nitrate-respiration
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose+builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from

antibiotic resistance

  • @ref: 122509
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 122509
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12250915688acetoin-
12250917234glucose-

enzymes

@refvalueactivityec
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
122509oxidase-
122509beta-galactosidase+3.2.1.23
122509alcohol dehydrogenase-1.1.1.1
122509gelatinase-
122509amylase-
122509DNase-
122509caseinase-3.4.21.50
122509catalase+1.11.1.6
122509tween esterase-
122509gamma-glutamyltransferase-2.3.2.2
122509lecithinase-
122509lipase-
122509lysine decarboxylase-4.1.1.18
122509ornithine decarboxylase-4.1.1.17
122509phenylalanine ammonia-lyase-4.3.1.24
122509protease-
122509tryptophan deaminase-
122509urease-3.5.1.5
68382beta-galactosidase-3.2.1.23

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122509--++-+----++---+++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122509+/-----+/---+/-+/-+/-++/--+/---++/-----+++++++/-+/-+/---+/-+/----+/-------+/--+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122509++-++--+-++++--++++--++--------+-+-+------------------------+--+----------------------+-+----------

Isolation, sampling and environmental information

isolation

@refsample type
8954activated sludge
122509Environment, Activated sludge

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Activated sludge

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89541Risk group (German classification)
1225091Risk group (French classification)

Sequence information

GC content

  • @ref: 8954
  • GC-content: 68.0
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 8954

culture collection no.: DSM 20606, IFO 15076, NBRC 15076, CIP 102894

straininfo link

  • @ref: 76827
  • straininfo: 8207

Reference

@idauthorscataloguedoi/urltitle
8954Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20606)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20606
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40472Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14630
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
76827Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8207.1StrainInfo: A central database for resolving microbial strain identifiers
122509Curators of the CIPCollection of Institut Pasteur (CIP 102894)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102894