Strain identifier

BacDive ID: 7308

Type strain: Yes

Species: Curtobacterium citreum

Strain Designation: 2Y-10

Culture col. no.: DSM 20528, ATCC 15828, CCM 2297, IAM 1614, NCIB 10702

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8895

BacDive-ID: 7308

DSM-Number: 20528

keywords: Bacteria, 16S sequence, genome sequence, mesophilic

description: Curtobacterium citreum 2Y-10 is a mesophilic bacterium that was isolated from paddy.

strain history: <- IAM <- K. Komagata, 2Y-10 (Brevibacterium citreum)

doi: 10.13145/bacdive7308.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Curtobacterium
  • species: Curtobacterium citreum
  • full scientific name: Curtobacterium citreum (Komagata and Iizuka 1964) Yamada and Komagata 1972 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Brevibacterium citreum

@ref: 8895

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Curtobacterium

species: Curtobacterium citreum

full scientific name: Curtobacterium citreum (Komagata and Iizuka 1964) Yamada and Komagata 1972

strain designation: 2Y-10

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18752Maize yellow10-14 daysISP 2
18752Zinc yellow10-14 daysISP 3
18752Light ivory10-14 daysISP 4
18752Oyster white10-14 daysISP 5
1875210-14 daysISP 6
18752Ivory10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18752noISP 2
18752noISP 3
18752noISP 4
18752noISP 5
18752noISP 6
18752noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18752ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18752ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18752ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18752ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18752ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18752ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
8895CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium53.pdf
37720MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)

culture temp

@refgrowthtypetemperaturerange
18752positiveoptimum28mesophilic
8895positivegrowth30mesophilic
37720positivegrowth30mesophilic

Physiology and metabolism

halophily

  • @ref: 18752
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

murein

  • @ref: 8895
  • murein short key: B05
  • type: B2ß {Gly} [L-Hsr] D-Glu-D-Orn

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18752+++-+++++++++-++-++

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18752+++-+++++++++-++-++

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18752+----------

Isolation, sampling and environmental information

isolation

  • @ref: 8895
  • sample type: paddy

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
187521Hazard group
88951Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Curtobacterium citreum gene for 16S ribosomal RNA, partial sequence, strain: DSM 20528AB915609946ena2036
20218Curtobacterium citreum partial 16S rRNA gene, type strain DSM 20528AM4106901472ena2036
20218Curtobacterium citreum 16S-23S ribosomal RNA intergenic spacer region, partial sequenceAY191507549ena2036
8895C.citreum (DSM 20528) 16S rRNA geneX774361472ena2036

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Curtobacterium citreum DSM 20528GCA_006715175contigncbi2036
66792Curtobacterium citreum NS330GCA_001475775contigncbi2036
66792Curtobacterium citreum strain DSM 205282036.6wgspatric2036
66792Curtobacterium citreum strain NS3302036.3wgspatric2036
66792Curtobacterium citreum DSM 205282806311065permanentimg2036

GC content

  • @ref: 8895
  • GC-content: 70.5

External links

@ref: 8895

culture collection no.: DSM 20528, ATCC 15828, CCM 2297, IAM 1614, NCIB 10702

straininfo link

@refpassport
20218http://www.straininfo.net/strains/40985
20218http://www.straininfo.net/strains/40983
20218http://www.straininfo.net/strains/40984
20218http://www.straininfo.net/strains/40986
20218http://www.straininfo.net/strains/40990

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8895Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20528)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20528
18752Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20528.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37720Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11185
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)