Strain identifier

BacDive ID: 7241

Type strain: Yes

Species: Methylophilus leisingeri

Strain Designation: DM11

Strain history: <- T. Leisinger, DM11 <- R. Scholtz

NCBI tax ID(s): 45393 (species)

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General

@ref: 2837

BacDive-ID: 7241

DSM-Number: 6813

keywords: 16S sequence, Bacteria

description: Methylophilus leisingeri DM11 is a bacterium that was isolated from groundwater.

NCBI tax id

  • NCBI tax id: 45393
  • Matching level: species

strain history

  • @ref: 2837
  • history: <- T. Leisinger, DM11 <- R. Scholtz

doi: 10.13145/bacdive7241.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Nitrosomonadales
  • family: Methylophilaceae
  • genus: Methylophilus
  • species: Methylophilus leisingeri
  • full scientific name: Methylophilus leisingeri corrig. Doronina and Trotsenko 2001

  • synonyms

    • @ref: 20215
    • synonym: Methylophilus leisingerii

@ref: 2837

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Methylophilales

family: Methylophilaceae

genus: Methylophilus

species: Methylophilus leisingeri

full scientific name: Methylophilus leisingeri Doronina and Trotsenko 2001

strain designation: DM11

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2837MINERAL MEDIUM WITH DICHLOROMETHANE (DSMZ Medium 465c)yeshttps://mediadive.dsmz.de/medium/465cName: MINERAL MEDIUM WITH DICHLOROMETHANE (DSMZ Medium 465c) Composition: Na2HPO4 x 2 H2O 3.5 g/l KH2PO4 1.0 g/l (NH4)2SO4 0.5 g/l MgCl2 x 6 H2O 0.1 g/l Ca(NO3)2 x 4 H2O 0.05 g/l EDTA 0.0005 g/l FeSO4 x 7 H2O 0.0002 g/l H3BO3 3e-05 g/l CoCl2 x 6 H2O 2e-05 g/l ZnSO4 x 7 H2O 1e-05 g/l MnCl2 x 4 H2O 3e-06 g/l Na2MoO4 x 2 H2O 3e-06 g/l NiCl2 x 6 H2O 2e-06 g/l CuCl2 x 2 H2O 1e-06 g/l Distilled water
2837NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 2837
  • growth: positive
  • type: growth
  • temperature: 30

Physiology and metabolism

compound production

  • @ref: 2837
  • compound: dichloromethane dehalogenase

Isolation, sampling and environmental information

isolation

  • @ref: 2837
  • sample type: groundwater

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Groundwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_4176.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_174;96_581;97_680;98_790;99_4176&stattab=map
  • Last taxonomy: Methylophilus
  • 16S sequence: AB193725
  • Sequence Identity:
  • Total samples: 1280
  • soil counts: 122
  • aquatic counts: 478
  • animal counts: 350
  • plant counts: 330

Safety information

risk assessment

  • @ref: 2837
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Methylophilus leisingerii 16S ribosomal RNA gene, partial sequenceAF2503331424nuccore45393
20218Methylophilus leisingeri gene for 16S rRNAAB1937251502nuccore45393

GC content

  • @ref: 2837
  • GC-content: 50.6±0.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 2837

culture collection no.: DSM 6813, VKM B-2013

straininfo link

  • @ref: 76693
  • straininfo: 48299

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2837Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6813)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6813
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76693Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48299.1StrainInfo: A central database for resolving microbial strain identifiers