Strain identifier
BacDive ID: 7219
Type strain: 
Species: Methylocystis bryophila
Strain Designation: H2s
Strain history: <- S. N. Dedysh, Winogradski Inst. Microbiol., RAS, Moscow, Russia; H2s <- S. E. Belova and S. N. Dedysh
NCBI tax ID(s): 655015 (species)
version 8.1 (current version)
General
@ref: 15991
BacDive-ID: 7219
DSM-Number: 21852
keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, coccus-shaped
description: Methylocystis bryophila H2s is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from Sphagnum peat, bank of a bog lake.
NCBI tax id
- NCBI tax id: 655015
- Matching level: species
strain history
- @ref: 15991
- history: <- S. N. Dedysh, Winogradski Inst. Microbiol., RAS, Moscow, Russia; H2s <- S. E. Belova and S. N. Dedysh
doi: 10.13145/bacdive7219.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Methylocystaceae
- genus: Methylocystis
- species: Methylocystis bryophila
- full scientific name: Methylocystis bryophila Belova et al. 2013
@ref: 15991
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Methylocystaceae
genus: Methylocystis
species: Methylocystis bryophila
full scientific name: Methylocystis bryophila Belova et al. 2013
strain designation: H2s
type strain: yes
Morphology
cell morphology
- @ref: 30743
- gram stain: negative
- cell length: 2.6 µm
- cell width: 1.15 µm
- cell shape: coccus-shaped
- motility: no
pigmentation
- @ref: 30743
- production: no
Culture and growth conditions
culture medium
- @ref: 15991
- name: METHYLOCYSTIS MEDIUM (DSMZ Medium 1409)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1409
- composition: Name: METHYLOCYSTIS MEDIUM (DSMZ Medium 1409) Composition: KNO3 0.2 g/l KH2PO4 0.1 g/l MgSO4 x 7 H2O 0.05 g/l CaCl2 x 2 H2O 0.01 g/l NaCl 0.01 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l CuCl2 x 5 H2O 0.0001 g/l Na2MoO4 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 15991 | positive | growth | 22 | psychrophilic |
| 30743 | positive | growth | 08-37 | |
| 30743 | positive | optimum | 27.5 | mesophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 30743 | positive | growth | 4.2-7.6 |
| 30743 | positive | optimum | 6.25 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 15991 | aerobe |
| 30743 | aerobe |
spore formation
- @ref: 30743
- spore formation: no
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30743 | 16449 | alanine | + | carbon source |
| 30743 | 22653 | asparagine | + | carbon source |
| 30743 | 29987 | glutamate | + | carbon source |
| 30743 | 26271 | proline | + | carbon source |
| 30743 | 17822 | serine | + | carbon source |
Isolation, sampling and environmental information
isolation
- @ref: 15991
- sample type: Sphagnum peat, bank of a bog lake
- host species: Sphagnum
- geographic location: north eastern Germany, lake Teufelssee
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
- Cat1: #Host
- Cat2: #Plants
- Cat3: #Peat moss
taxonmaps
- @ref: 69479
- File name: preview.99_3222.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_208;97_227;98_2469;99_3222&stattab=map
- Last taxonomy: Methylocystis bryophila subclade
- 16S sequence: FN422003
- Sequence Identity:
- Total samples: 500
- soil counts: 210
- aquatic counts: 249
- animal counts: 29
- plant counts: 12
Safety information
risk assessment
- @ref: 15991
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15991
- description: Methylocystis bryophila partial 16S rRNA gene, type strain H2sT
- accession: FN422003
- length: 1427
- database: ena
- NCBI tax ID: 655015
GC content
| @ref | GC-content | method |
|---|---|---|
| 15991 | 62 | thermal denaturation, midpoint method (Tm) |
| 30743 | 62 |
External links
@ref: 15991
culture collection no.: DSM 21852, VKM B-2545
straininfo link
- @ref: 76671
- straininfo: 403670
literature
- topic: Phylogeny
- Pubmed-ID: 22707532
- title: Methylocystis bryophila sp. nov., a facultatively methanotrophic bacterium from acidic Sphagnum peat, and emended description of the genus Methylocystis (ex Whittenbury et al. 1970) Bowman et al. 1993.
- authors: Belova SE, Kulichevskaya IS, Bodelier PLE, Dedysh SN
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.043505-0
- year: 2012
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids, Genes, Bacterial, Germany, Hydrogen-Ion Concentration, Methane/metabolism, Methylocystaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nitrogen Fixation, Oxygenases/genetics, *Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, *Soil Microbiology, Sphagnopsida/*microbiology, Wetlands
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 15991 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21852) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21852 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 30743 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27074 | 28776041 | ||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 76671 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403670.1 | StrainInfo: A central database for resolving microbial strain identifiers |