Strain identifier

BacDive ID: 7134

Type strain: No

Species: Methanospirillum hungatei

Strain Designation: SK

Strain history: <- F. Widdel, University of Illinois, Urbana, USA; SK <- F. Widdel {1984}

NCBI tax ID(s): 2203 (species)

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General

@ref: 1418

BacDive-ID: 7134

DSM-Number: 3595

keywords: Archaea, anaerobe, methane production

description: Methanospirillum hungatei SK is an anaerobe archaeon that produces methane and was isolated from ditch mud.

NCBI tax id

  • NCBI tax id: 2203
  • Matching level: species

strain history

  • @ref: 1418
  • history: <- F. Widdel, University of Illinois, Urbana, USA; SK <- F. Widdel {1984}

doi: 10.13145/bacdive7134.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanomicrobiales
  • family: Methanospirillaceae
  • genus: Methanospirillum
  • species: Methanospirillum hungatei
  • full scientific name: Methanospirillum hungatei corrig. Ferry et al. 1974 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Methanospirillum hungatii

@ref: 1418

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanospirillaceae

genus: Methanospirillum

species: Methanospirillum hungatei

full scientific name: Methanospirillum hungatei Ferry et al. 1974 emend. Iino et al. 2010

strain designation: SK

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1418MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523)yeshttps://mediadive.dsmz.de/medium/1523Name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523) Composition: Brain heart infusion 5.98802 g/l Proteose peptone 5.98802 g/l NaHCO3 3.99202 g/l Yeast extract 1.99601 g/l Na-formate 1.99601 g/l Na-acetate 0.998004 g/l Na2S x 9 H2O 0.499002 g/l KH2PO4 0.499002 g/l L-Cysteine HCl x H2O 0.499002 g/l MgSO4 x 7 H2O 0.399202 g/l NH4Cl 0.399202 g/l NaCl 0.399202 g/l CaCl2 x 2 H2O 0.0499002 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 0.000299401 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l CoCl2 x 6 H2O 0.000189621 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l ZnCl2 6.98603e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l D-(+)-biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water
1418METHANOBACTERIUM MEDIUM (DSMZ Medium 119)yeshttps://mediadive.dsmz.de/medium/119Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
1418METHANOSPIRILLUM SK MEDIUM (DSMZ Medium 374)yeshttps://mediadive.dsmz.de/medium/374Name: METHANOSPIRILLUM SK MEDIUM (DSMZ Medium 374) Composition: NaHCO3 3.996 g/l Na-formate 1.998 g/l Na-acetate 0.999001 g/l Na2S x 9 H2O 0.499501 g/l KH2PO4 0.499501 g/l NH4Cl 0.3996 g/l MgSO4 x 7 H2O 0.3996 g/l NaCl 0.3996 g/l Yeast extract 0.1998 g/l CaCl2 x 2 H2O 0.04995 g/l HCl 0.0024975 g/l FeSO4 x 7 H2O 0.001998 g/l FeCl2 x 4 H2O 0.0014985 g/l Sodium resazurin 0.0004995 g/l CoCl2 x 6 H2O 0.00018981 g/l MnCl2 x 4 H2O 9.99001e-05 g/l ZnCl2 6.99301e-05 g/l Na2MoO4 x 2 H2O 3.5964e-05 g/l NiCl2 x 6 H2O 2.3976e-05 g/l H3BO3 5.99401e-06 g/l CuCl2 x 2 H2O 1.998e-06 g/l Isopropyl alcohol Valeric acid DL-2-Methylbutyric acid Isobutyric acid Sludge Isovaleric acid Distilled water

culture temp

  • @ref: 1418
  • growth: positive
  • type: growth
  • temperature: 37

Physiology and metabolism

oxygen tolerance

  • @ref: 1418
  • oxygen tolerance: anaerobe

compound production

  • @ref: 1418
  • compound: methane

Isolation, sampling and environmental information

isolation

  • @ref: 1418
  • sample type: ditch mud
  • geographic location: Konstanz
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Mud (Sludge)

Safety information

risk assessment

  • @ref: 1418
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

GC content

  • @ref: 1418
  • GC-content: 39.4

External links

@ref: 1418

culture collection no.: DSM 3595

straininfo link

  • @ref: 76588
  • straininfo: 47730

Reference

@idauthorscataloguedoi/urltitle
1418Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3595)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3595
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
76588Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47730.1StrainInfo: A central database for resolving microbial strain identifiers