Strain identifier
BacDive ID: 7132
Type strain: 
Species: Methanospirillum hungatei
Strain Designation: JF-1, JF1
Strain history: DSM 864 <-- M. P. Bryant <-- J. G. Ferry JF1.
NCBI tax ID(s): 323259 (strain), 2203 (species)
version 8.1 (current version)
General
@ref: 467
BacDive-ID: 7132
DSM-Number: 864
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanospirillum hungatei JF-1 is an anaerobe, mesophilic archaeon that was isolated from sewage sludge.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 2203 | species |
| 323259 | strain |
strain history
| @ref | history |
|---|---|
| 467 | <- M.P. Bryant <- J.G. Ferry, JF1 (Methanospirillum hungatii, sic.) |
| 67770 | DSM 864 <-- M. P. Bryant <-- J. G. Ferry JF1. |
doi: 10.13145/bacdive7132.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanomicrobiales
- family: Methanospirillaceae
- genus: Methanospirillum
- species: Methanospirillum hungatei
- full scientific name: Methanospirillum hungatei corrig. Ferry et al. 1974 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Methanospirillum hungatii
@ref: 467
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanomicrobiales
family: Methanospirillaceae
genus: Methanospirillum
species: Methanospirillum hungatei
full scientific name: Methanospirillum hungatei Ferry et al. 1974 emend. Iino et al. 2010
strain designation: JF-1, JF1
type strain: yes
Culture and growth conditions
culture medium
- @ref: 467
- name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119
- composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 467 | positive | growth | 37 | mesophilic |
| 67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 467
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 467 | sewage sludge | USA | USA | North America |
| 67770 | Sewage sludge |
isolation source categories
- Cat1: #Engineered
- Cat2: #Waste
- Cat3: #Sewage sludge
taxonmaps
- @ref: 69479
- File name: preview.99_187089.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17807;96_68777;97_89419;98_121688;99_187089&stattab=map
- Last taxonomy: Methanospirillum hungatei subclade
- 16S sequence: M60880
- Sequence Identity:
- Total samples: 770
- soil counts: 36
- aquatic counts: 628
- animal counts: 20
- plant counts: 86
Safety information
risk assessment
- @ref: 467
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Enterococcus faecalis clone OCM016 16S ribosomal RNA gene, partial sequence | GU417217 | 467 | ena | 1351 |
| 20218 | Methanospirillum hungatei strain JF-1 16S ribosomal RNA gene, partial sequence | JQ346747 | 1271 | ena | 323259 |
| 20218 | M.hungatei 16S ribosomal RNA | M60880 | 1466 | ena | 2203 |
| 67770 | Methanospirillum hungatei gene for 16S ribosomal RNA, partial sequence | AB517987 | 1259 | ena | 323259 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanospirillum hungatei JF-1 | GCA_000013445 | complete | ncbi | 323259 |
| 66792 | Methanospirillum hungatei JF-1 | 637000164 | complete | img | 323259 |
| 66792 | Methanospirillum hungatei JF-1 | 323259.15 | complete | patric | 323259 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 467 | 45.0 | |
| 67770 | 45 | Buoyant density centrifugation (BD) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 84.958 | no |
| flagellated | no | 96.261 | no |
| gram-positive | no | 97.613 | no |
| anaerobic | yes | 99.162 | yes |
| halophile | no | 93.955 | no |
| spore-forming | no | 95.122 | no |
| glucose-util | yes | 68.97 | no |
| aerobic | no | 96.24 | yes |
| thermophile | no | 89.407 | no |
| glucose-ferment | no | 59.58 | no |
External links
@ref: 467
culture collection no.: DSM 864, ATCC 27890, JCM 10133, OCM 16, NBRC 100397
straininfo link
- @ref: 76587
- straininfo: 47281
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 8837434 | Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. | Arahal DR, Dewhirst FE, Paster BJ, Volcani BE, Ventosa A | Appl Environ Microbiol | 10.1128/aem.62.10.3779-3786.1996 | 1996 | Archaea/*genetics/growth & development/metabolism, DNA, Bacterial/analysis, Genes, Bacterial/genetics, Israel, Molecular Sequence Data, Phenotype, *Phylogeny, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Water Microbiology | Genetics |
| Phylogeny | 10758870 | Desulfovirga adipica gen. nov., sp. nov., an adipate-degrading, gram-negative, sulfate-reducing bacterium. | Tanaka K, Stackebrandt E, Tohyama S, Eguchi T | Int J Syst Evol Microbiol | 10.1099/00207713-50-2-639 | 2000 | Adipates/*metabolism, Anaerobiosis, Base Composition, Culture Media, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/cytology/isolation & purification/physiology, Industrial Waste, Molecular Sequence Data, Phylogeny, Pigments, Biological, RNA, Ribosomal, 16S/genetics, Sulfur-Reducing Bacteria/*classification/cytology/isolation & purification/physiology, Waste Disposal, Fluid, Water Microbiology | Cultivation |
| Phylogeny | 12656175 | Victivallis vadensis gen. nov., sp. nov., a sugar-fermenting anaerobe from human faeces. | Zoetendal EG, Plugge CM, Akkermans ADL, de Vos WM | Int J Syst Evol Microbiol | 10.1099/ijs.0.02362-0 | 2003 | Base Composition, Carbohydrate Metabolism, Cellobiose/metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Feces/microbiology, Fermentation, Gram-Negative Anaerobic Cocci/*classification/genetics/isolation & purification/*metabolism, Humans, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics | Metabolism |
| Phylogeny | 16014504 | The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum. | de Bok FAM, Harmsen HJM, Plugge CM, de Vries MC, Akkermans ADL, de Vos WM, Stams AJM | Int J Syst Evol Microbiol | 10.1099/ijs.0.02880-0 | 2005 | Anaerobiosis, *Bioreactors, Culture Media, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, In Situ Hybridization, Fluorescence, Industrial Waste, Methanospirillum/*growth & development, Molecular Sequence Data, Oxidation-Reduction, Peptococcaceae/*classification/genetics/*growth & development/isolation & purification, Phenotype, Propionates/*metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Disposal, Fluid | Cultivation |
| 16349240 | Regulation of Product Formation in Bacteroides xylanolyticus X5-1 by Interspecies Electron Transfer. | Biesterveld S, Zehnder AJ, Stams AJ | Appl Environ Microbiol | 10.1128/aem.60.4.1347-1352.1994 | 1994 | |||
| Phylogeny | 19495416 | Genomic characterization of methanomicrobiales reveals three classes of methanogens. | Anderson I, Ulrich LE, Lupa B, Susanti D, Porat I, Hooper SD, Lykidis A, Sieprawska-Lupa M, Dharmarajan L, Goltsman E, Lapidus A, Saunders E, Han C, Land M, Lucas S, Mukhopadhyay B, Whitman WB, Woese C, Bristow J, Kyrpides N | PLoS One | 10.1371/journal.pone.0005797 | 2009 | Archaea/metabolism, Archaeal Proteins/metabolism, Classification, Cluster Analysis, Genetic Techniques, Genome, Archaeal, *Genomics, Methanomicrobiales/classification/*genetics, Models, Biological, Phylogeny, Sequence Analysis, DNA, Sigma Factor/genetics | Genetics |
| Phylogeny | 20008107 | Methanospirillum lacunae sp. nov., a methane-producing archaeon isolated from a puddly soil, and emended descriptions of the genus Methanospirillum and Methanospirillum hungatei. | Iino T, Mori K, Suzuki KI | Int J Syst Evol Microbiol | 10.1099/ijs.0.020131-0 | 2009 | Base Composition, DNA, Archaeal/genetics, Methane/*metabolism, Methanospirillum/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology | Metabolism |
| Metabolism | 21798981 | Metabolism of H2 by Desulfovibrio alaskensis G20 during syntrophic growth on lactate. | Li X, McInerney MJ, Stahl DA, Krumholz LR | Microbiology (Reading) | 10.1099/mic.0.051284-0 | 2011 | Bacterial Proteins/genetics/metabolism, Desulfovibrio/genetics/*growth & development/*metabolism, Gene Expression Regulation, Bacterial, Hydrogen/*metabolism, Lactic Acid/*metabolism | |
| Metabolism | 23934484 | Mercury methylation by the methanogen Methanospirillum hungatei. | Yu RQ, Reinfelder JR, Hines ME, Barkay T | Appl Environ Microbiol | 10.1128/AEM.01556-13 | 2013 | Archaeal Proteins/genetics/metabolism, Cluster Analysis, Culture Media/chemistry, Mercury/*metabolism, Methanospirillum/genetics/*metabolism, Methylation, Methylmercury Compounds/*metabolism, Phylogeny, Sequence Analysis, DNA, Sulfides/metabolism | Phylogeny |
| Phylogeny | 24676730 | Lactivibrio alcoholicus gen. nov., sp. nov., an anaerobic, mesophilic, lactate-, alcohol-, carbohydrate- and amino-acid-degrading bacterium in the phylum Synergistetes. | Qiu YL, Hanada S, Kamagata Y, Guo RB, Sekiguchi Y | Int J Syst Evol Microbiol | 10.1099/ijs.0.060681-0 | 2014 | Alcohols/metabolism, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/genetics/isolation & purification, Lactic Acid/metabolism, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Disposal, Fluid | Metabolism |
| Phylogeny | 26637817 | Isolation and characterization of Flexilinea flocculi gen. nov., sp. nov., a filamentous, anaerobic bacterium belonging to the class Anaerolineae in the phylum Chloroflexi. | Sun L, Toyonaga M, Ohashi A, Matsuura N, Tourlousse DM, Meng XY, Tamaki H, Hanada S, Cruz R, Yamaguchi T, Sekiguchi Y | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000822 | 2015 | ||
| Genetics | 26744606 | Complete genome sequence of Methanospirillum hungatei type strain JF1. | Gunsalus RP, Cook LE, Crable B, Rohlin L, McDonald E, Mouttaki H, Sieber JR, Poweleit N, Zhou H, Lapidus AL, Daligault HE, Land M, Gilna P, Ivanova N, Kyrpides N, Culley DE, McInerney MJ | Stand Genomic Sci | 10.1186/s40793-015-0124-8 | 2016 | ||
| 30483237 | The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein. | Christensen LFB, Hansen LM, Finster K, Christiansen G, Nielsen PH, Otzen DE, Dueholm MS | Front Microbiol | 10.3389/fmicb.2018.02729 | 2018 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 467 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 864) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-864 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 76587 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47281.1 | StrainInfo: A central database for resolving microbial strain identifiers |