Strain identifier
BacDive ID: 7118
Type strain: 
Species: Methanosarcina sp.
Strain Designation: Ms 97
Strain history: <- E. Weigand, Univ. Giessen, Germany; Ms 97 <- S. Pfitzer {1997}
NCBI tax ID(s): 1520800 (species)
version 8.1 (current version)
General
@ref: 4513
BacDive-ID: 7118
DSM-Number: 11855
keywords: genome sequence, Archaea, anaerobe, thermophilic
description: Methanosarcina sp. Ms 97 is an anaerobe, thermophilic archaeon that was isolated from ovine rumen.
NCBI tax id
- NCBI tax id: 1520800
- Matching level: species
strain history
- @ref: 4513
- history: <- E. Weigand, Univ. Giessen, Germany; Ms 97 <- S. Pfitzer {1997}
doi: 10.13145/bacdive7118.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina sp.
- full scientific name: Methanosarcina Kluyver and van Niel 1936 (Approved Lists 1980)
@ref: 4513
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina sp.
full scientific name: Methanosarcina sp.
strain designation: Ms 97
type strain: no
Culture and growth conditions
culture medium
- @ref: 4513
- name: METHANOSARCINA MEDIUM (DSMZ Medium 120)
- growth: yes
- link: https://mediadive.dsmz.de/medium/120
- composition: Name: METHANOSARCINA MEDIUM (DSMZ Medium 120; with strain-specific modifications) Composition: Methanol 7.92 g/l Na-acetate 2.5 g/l NaCl 2.25 g/l Casitone 2.0 g/l NaHCO3 2.0 g/l Yeast extract 2.0 g/l NH4Cl 0.5 g/l MgSO4 x 7 H2O 0.5 g/l K2HPO4 0.35 g/l Na2S x 9 H2O 0.3 g/l L-Cysteine HCl x H2O 0.3 g/l CaCl2 x 2 H2O 0.25 g/l KH2PO4 0.23 g/l HCl 0.0025 g/l FeSO4 x 7 H2O 0.002 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l ZnCl2 7e-05 g/l (DL)-alpha-Lipoic acid 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Thiamine HCl 5e-05 g/l Riboflavin 5e-05 g/l Nicotinic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Rumen fluid Distilled water
culture temp
- @ref: 4513
- growth: positive
- type: growth
- temperature: 50
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 4513
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 4513
- sample type: ovine rumen
- geographic location: Hessen, Giessen
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Caprinae (Sheep/Goat) |
| #Host Body-Site | #Organ | #Rumen |
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanosarcina thermophila DSM 11855 | GCA_900116525 | scaffold | ncbi | 2210 |
| 66792 | Methanosarcina sp. DSM 11855 | 2595698250 | draft | img | 1520800 |
| 66792 | Methanosarcina sp. DSM 11855 | 1520800.3 | wgs | patric | 1520800 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 88.882 | no |
| flagellated | no | 97.644 | no |
| gram-positive | no | 95.166 | no |
| anaerobic | yes | 96.872 | yes |
| aerobic | no | 93.863 | no |
| halophile | no | 87.454 | no |
| spore-forming | no | 95.298 | no |
| glucose-ferment | no | 70.101 | no |
| thermophile | no | 68.917 | yes |
| glucose-util | yes | 67.949 | no |
External links
@ref: 4513
culture collection no.: DSM 11855
straininfo link
- @ref: 76573
- straininfo: 407751
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4513 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11855) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11855 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 76573 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID407751.1 | StrainInfo: A central database for resolving microbial strain identifiers |