Strain identifier
BacDive ID: 7085
Type strain:
Species: Methanosarcina siciliae
Strain Designation: C2J
Strain history: <- OCM <- M.A. Elberson and K.R. Sowers, C2J
NCBI tax ID(s): 38027 (species)
General
@ref: 4537
BacDive-ID: 7085
DSM-Number: 11916
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanosarcina siciliae C2J is an anaerobe, mesophilic archaeon that was isolated from marine sediment.
NCBI tax id
- NCBI tax id: 38027
- Matching level: species
strain history
- @ref: 4537
- history: <- OCM <- M.A. Elberson and K.R. Sowers, C2J
doi: 10.13145/bacdive7085.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina siciliae
- full scientific name: Methanosarcina siciliae (Stetter and König 1989) Ni et al. 1994
synonyms
- @ref: 20215
- synonym: Methanolobus siciliae
@ref: 4537
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina siciliae
full scientific name: Methanosarcina siciliae (Stetter and König 1989) Ni et al. 1994 emend. Elberson and Sowers 1997
strain designation: C2J
type strain: no
Culture and growth conditions
culture medium
- @ref: 4537
- name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/141c
- composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c) Composition: NaHCO3 4.89237 g/l Trimethylammonium chloride 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water
culture temp
- @ref: 4537
- growth: positive
- type: growth
- temperature: 35
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 4537
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 4537
- sample type: marine sediment
- geographic location: California, Scripps Canyon near La Jolla
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Environmental | #Aquatic | #Sediment |
Safety information
risk assessment
- @ref: 4537
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4537
- description: Methnosarcina siciliae 16S ribosomal RNA gene, partial sequence
- accession: U89773
- length: 1350
- database: ena
- NCBI tax ID: 38027
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Methanosarcina siciliae C2J | GCA_000970145 | complete | ncbi | 1434118 |
66792 | Methanosarcina siciliae C2J | 2627853753 | complete | img | 1434118 |
66792 | Methanosarcina siciliae C2J | 1434118.4 | complete | patric | 1434118 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 92.158 | no |
flagellated | no | 97.177 | no |
gram-positive | no | 96.044 | no |
anaerobic | yes | 98.117 | yes |
aerobic | no | 94.689 | no |
halophile | no | 88.23 | no |
spore-forming | no | 94.787 | no |
thermophile | no | 99.085 | yes |
glucose-util | yes | 70.962 | no |
glucose-ferment | no | 63.713 | no |
External links
@ref: 4537
culture collection no.: DSM 11916, OCM 653
straininfo link
- @ref: 76541
- straininfo: 49206
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
4537 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11916) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11916 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
76541 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49206.1 | StrainInfo: A central database for resolving microbial strain identifiers |