Strain identifier
BacDive ID: 7084
Type strain: 
Species: Methanosarcina siciliae
Strain Designation: HI350
Strain history: <- OCM <- S. Ni, strain HI350
NCBI tax ID(s): 38027 (species)
version 8.1 (current version)
General
@ref: 2731
BacDive-ID: 7084
DSM-Number: 6564
keywords: genome sequence, Archaea, anaerobe, mesophilic
description: Methanosarcina siciliae HI350 is an anaerobe, mesophilic archaeon that was isolated from production water from an off-shore oil well.
NCBI tax id
- NCBI tax id: 38027
- Matching level: species
strain history
- @ref: 2731
- history: <- OCM <- S. Ni, strain HI350
doi: 10.13145/bacdive7084.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina siciliae
- full scientific name: Methanosarcina siciliae (Stetter and König 1989) Ni et al. 1994
synonyms
- @ref: 20215
- synonym: Methanolobus siciliae
@ref: 2731
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina siciliae
full scientific name: Methanosarcina siciliae (Stetter and König 1989) Ni et al. 1994 emend. Elberson and Sowers 1997
strain designation: HI350
type strain: no
Culture and growth conditions
culture medium
- @ref: 2731
- name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/141c
- composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c) Composition: NaHCO3 4.89237 g/l Trimethylammonium chloride 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water
culture temp
- @ref: 2731
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 2731
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 2731
- sample type: production water from an off-shore oil well
- geographic location: Gulf of Mexico
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | #Oil reservoir |
| #Environmental | #Aquatic |
Safety information
risk assessment
- @ref: 2731
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanosarcina siciliae HI350 | GCA_000970125 | complete | ncbi | 1434119 |
| 66792 | Methanosarcina siciliae HI350 | 2645727536 | complete | img | 1434119 |
| 66792 | Methanosarcina siciliae HI350 | 1434119.4 | complete | patric | 1434119 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 92.167 | no |
| flagellated | no | 97.773 | no |
| gram-positive | no | 95.98 | no |
| anaerobic | yes | 97.833 | yes |
| aerobic | no | 93.878 | yes |
| halophile | no | 88.128 | no |
| spore-forming | no | 95.522 | no |
| thermophile | no | 98.158 | yes |
| glucose-util | yes | 73.66 | no |
| glucose-ferment | no | 65.078 | no |
External links
@ref: 2731
culture collection no.: DSM 6564, OCM 210
straininfo link
- @ref: 76540
- straininfo: 48237
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 1883716 | Isolation and characterization of a dimethyl sulfide-degrading methanogen, Methanolobus siciliae HI350, from an oil well, characterization of M. siciliae T4/MT, and emendation of M. siciliae. | Ni SS, Boone DR | Int J Syst Bacteriol | 10.1099/00207713-41-3-410 | 1991 | Base Composition, DNA, Bacterial, Euryarchaeota/classification/*isolation & purification/metabolism, Methane/metabolism, *Petroleum, Sulfides/*metabolism, Temperature | Metabolism |
| Phylogeny | 11536640 | Transfer of Methanolobus siciliae to the genus Methanosarcina, naming it Methanosarcina siciliae, and emendation of the genus Methanosarcina. | Ni S, Woese CR, Aldrich HC, Boone DR | Int J Syst Bacteriol | 10.1099/00207713-44-2-357 | 1994 | Methanosarcina/*classification/genetics/ultrastructure, Methanosarcinaceae/*classification/genetics/ultrastructure, Microscopy, Electron, *Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S/*classification/genetics, Sequence Analysis, RNA, *Sequence Homology, Nucleic Acid | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 2731 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6564) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6564 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 76540 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48237.1 | StrainInfo: A central database for resolving microbial strain identifiers |