Strain identifier

BacDive ID: 7077

Type strain: Yes

Species: Methanolobus vulcani

Strain Designation: PL-12/M

Strain history: <- K.O. Stetter, PL-12/M

NCBI tax ID(s): 38026 (species)

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General

@ref: 1275

BacDive-ID: 7077

DSM-Number: 3029

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanolobus vulcani PL-12/M is an anaerobe, mesophilic archaeon that was isolated from submarine fumarole.

NCBI tax id

  • NCBI tax id: 38026
  • Matching level: species

strain history

  • @ref: 1275
  • history: <- K.O. Stetter, PL-12/M

doi: 10.13145/bacdive7077.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanolobus
  • species: Methanolobus vulcani
  • full scientific name: Methanolobus vulcani Stetter et al. 1989

@ref: 1275

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanolobus

species: Methanolobus vulcani

full scientific name: Methanolobus vulcani Stetter et al. 1989 emend. Kadam and Boone 1995

strain designation: PL-12/M

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 1275
  • name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/141c
  • composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Methanol 3.87476 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

culture temp

  • @ref: 1275
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1275
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1275
  • sample type: submarine fumarole
  • geographic location: Vulcano island, Porto di Levante
  • country: Italy
  • origin.country: ITA
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Volcanic

Safety information

risk assessment

  • @ref: 1275
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 1275
  • description: Methanolobus vulcani 16S small subunit ribosomal RNA gene, partial sequence
  • accession: U20155
  • length: 1419
  • database: ena
  • NCBI tax ID: 38026

GC content

  • @ref: 1275
  • GC-content: 39.0

External links

@ref: 1275

culture collection no.: DSM 3029, OCM 157

straininfo link

  • @ref: 76533
  • straininfo: 47686

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism11972621Reduction of Fe(III) oxide by methanogens in the presence and absence of extracellular quinones.Bond DR, Lovley DREnviron Microbiol10.1046/j.1462-2920.2002.00279.x2002Anthraquinones/*pharmacology, Euryarchaeota/growth & development/*metabolism, Ferric Compounds/*metabolism, Humic Substances/pharmacology, Oxidation-Reduction/drug effects, Species SpecificityPhylogeny
Phylogeny19329594Methanolobus profundi sp. nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field.Mochimaru H, Tamaki H, Hanada S, Imachi H, Nakamura K, Sakata S, Kamagata YInt J Syst Evol Microbiol10.1099/ijs.0.001677-02009Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Geologic Sediments/*microbiology, Japan, Magnesium/metabolism, Methane/*metabolism, Methanol/metabolism, Methanosarcinaceae/*classification/genetics/*isolation & purification/metabolism, Methylamines/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium/metabolismMetabolism
Phylogeny25319587Isolation and characterization of a tetramethylammonium-degrading Methanococcoides strain and a novel glycine betaine-utilizing Methanolobus strain.Ticak T, Hariraju D, Arcelay MB, Arivett BA, Fiester SE, Ferguson DJ JrArch Microbiol10.1007/s00203-014-1043-62014Betaine/*metabolism, Geologic Sediments/microbiology, Methanosarcinaceae/*classification/genetics/isolation & purification/*physiology, *Phylogeny, Quaternary Ammonium Compounds/*metabolism, RNA, Ribosomal, 16S/geneticsMetabolism

Reference

@idauthorscataloguedoi/urltitle
1275Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3029)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3029
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
76533Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47686.1StrainInfo: A central database for resolving microbial strain identifiers