General

@ref: 3928

BacDive-ID: 7075

DSM-Number: 10369

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanohalophilus euhalobius 283 is an anaerobe, mesophilic archaeon that was isolated from saline subsurface water, oil field.

NCBI tax id

• NCBI tax id: 51203
• Matching level: species

strain history

• @ref: 3928
• history: <- I. A. Davidova; 283 (Methanococcoides euhalobius) <- A. Y. Obraztsova {1984}

doi: 10.13145/bacdive7075.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanosarcinales
• family: Methanosarcinaceae
• genus: Methanohalophilus
• species: Methanohalophilus euhalobius
• full scientific name: Methanohalophilus euhalobius Davidova et al. 1997

@ref: 3928

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanohalophilus

species: Methanohalophilus euhalobius

full scientific name: Methanohalophilus euhalobius

strain designation: 283

type strain: no

Culture and growth conditions

culture medium

• @ref: 3928
• name: METHANOHALOPHILUS EUHALOBIUS MEDIUM (DSMZ Medium 713)
• growth: yes
• link: https://mediadive.dsmz.de/medium/713
• composition: Name: METHANOHALOPHILUS EUHALOBIUS MEDIUM (DSMZ Medium 713) Composition: Trimethylamine-HCl 10.0 g/l MgCl2 x 6 H2O 2.4 g/l CaCl2 x 2 H2O 2.0 g/l Yeast extract 2.0 g/l Na2CO3 1.0 g/l KCl 1.0 g/l Na2-EDTA 0.5 g/l NH4Cl 0.5 g/l L-Cysteine HCl x H2O 0.5 g/l Na2S x 9 H2O 0.5 g/l KH2PO4 0.4 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l ZnSO4 x 7 H2O 0.0018 g/l CoSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l Pyridoxine hydrochloride 5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l Riboflavin 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Folic acid 1e-05 g/l Biotin 1e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Vitamin B12 5e-07 g/l Distilled water

culture temp

• @ref: 3928
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 3928
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 3928
• sample type: saline subsurface water, oil field
• geographic location: Bonduzhkoe oil deposit
• country: Russia
• origin.country: RUS

isolation source categories

Safety information

risk assessment

• @ref: 3928
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 3928
• description: M.euhalobius 16S rRNA gene
• accession: X98192
• length: 1337
• database: ena
• NCBI tax ID: 51203

Genome sequences

GC content

• @ref: 3928
• GC-content: 43
• method: thermal denaturation, midpoint method (Tm)

External links

@ref: 3928

culture collection no.: DSM 10369

Reference