Strain identifier
BacDive ID: 7069
Type strain: 
Species: Methanohalophilus portucalensis
Strain Designation: SF1
Strain history: <- D.R. Boone, SF1 <- R.H. Mah
NCBI tax ID(s): 39664 (species)
version 8.1 (current version)
General
@ref: 1324
BacDive-ID: 7069
DSM-Number: 3243
keywords: 16S sequence, Archaea, anaerobe, mesophilic
description: Methanohalophilus portucalensis SF1 is an anaerobe, mesophilic archaeon that was isolated from solar salt pond.
NCBI tax id
- NCBI tax id: 39664
- Matching level: species
strain history
- @ref: 1324
- history: <- D.R. Boone, SF1 <- R.H. Mah
doi: 10.13145/bacdive7069.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanohalophilus
- species: Methanohalophilus portucalensis
- full scientific name: Methanohalophilus portucalensis Boone et al. 1993
@ref: 1324
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanohalophilus
species: Methanohalophilus portucalensis
full scientific name: Methanohalophilus portucalensis Boone et al. 1993
strain designation: SF1
type strain: no
Culture and growth conditions
culture medium
- @ref: 1324
- name: METHANOHALOPHILUS PORTUCALENSIS MEDIUM (DSMZ Medium 348)
- growth: yes
- link: https://mediadive.dsmz.de/medium/348
- composition: Name: METHANOHALOPHILUS PORTUCALENSIS MEDIUM (DSMZ Medium 348) Composition: NaCl 120.0 g/l MgCl2 x 6 H2O 7.0 g/l MgSO4 x 7 H2O 6.0 g/l KCl 3.8 g/l Yeast extract 2.0 g/l Trypticase peptone 2.0 g/l Trimethylamine-HCl 1.9 g/l Na2CO3 1.5 g/l NH4Cl 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l CaCl2 x 2 H2O 0.5 g/l K2HPO4 x 3 H2O 0.4 g/l Na2S x 9 H2O 0.25 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l NaOH 0.0005 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
culture temp
- @ref: 1324
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1324
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 1324
- sample type: solar salt pond
- geographic location: California, San Francisco, Leslie Salt Co.
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Pond (small) |
| #Condition | #Saline |
Safety information
risk assessment
- @ref: 1324
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Lactobacillus paracasei clone OCM013 16S ribosomal RNA gene, partial sequence | GU425026 | 467 | ena | 1597 |
| 20218 | Methanohalophilus portucalensis 16S ribosomal RNA gene, partial sequence | M59132 | 1425 | ena | 39664 |
| 20218 | Bdellovibrio bacteriovorus 16S ribosomal RNA | M59297 | 1516 | ena | 959 |
GC content
- @ref: 1324
- GC-content: 41.0
External links
@ref: 1324
culture collection no.: DSM 3243, OCM 13
straininfo link
- @ref: 76526
- straininfo: 47705
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1324 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3243) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3243 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 76526 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47705.1 | StrainInfo: A central database for resolving microbial strain identifiers |