Strain identifier

BacDive ID: 7062

Type strain: Yes

Species: Methanohalobium evestigatum

Strain Designation: Z-7303

Strain history: <- T.N. Zhilina, Z-7303

NCBI tax ID(s): 2322 (species)

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General

@ref: 1397

BacDive-ID: 7062

DSM-Number: 3721

keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic

description: Methanohalobium evestigatum Z-7303 is an anaerobe, thermophilic archaeon that was isolated from mud from salt lagoon.

NCBI tax id

  • NCBI tax id: 2322
  • Matching level: species

strain history

  • @ref: 1397
  • history: <- T.N. Zhilina, Z-7303

doi: 10.13145/bacdive7062.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanohalobium
  • species: Methanohalobium evestigatum
  • full scientific name: Methanohalobium evestigatum corrig. Zhilina and Zavarzin 1988

  • synonyms

    • @ref: 20215
    • synonym: Methanohalobium evestigatus

@ref: 1397

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanohalobium

species: Methanohalobium evestigatum

full scientific name: Methanohalobium evestigatum Zhilina and Zavarzin 1988

strain designation: Z-7303

type strain: yes

Morphology

colony morphology

  • @ref: 1397
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 1397
  • name: METHANOHALOBIUM MEDIUM (DSMZ Medium 375)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/375
  • composition: Name: METHANOHALOBIUM MEDIUM (DSMZ Medium 375) Composition: NaCl 250.0 g/l Trimethylamine-HCl 5.0 g/l MgSO4 x 7 H2O 4.0 g/l Na2CO3 1.5 g/l Na2S x 9 H2O 0.5 g/l CaCl2 x 2 H2O 0.33 g/l KH2PO4 0.33 g/l MgCl2 x 6 H2O 0.33 g/l KCl 0.33 g/l NH4Cl 0.33 g/l Yeast extract 0.05 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water

culture temp

  • @ref: 1397
  • growth: positive
  • type: growth
  • temperature: 50
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1397
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1397
  • sample type: mud from salt lagoon
  • geographic location: Crimea
  • country: Ukraine
  • origin.country: UKR
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic
#Environmental#Terrestrial#Mud (Sludge)
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_187200.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17878;96_68854;97_89501;98_121782;99_187200&stattab=map
  • Last taxonomy: Methanohalobium evestigatum subclade
  • 16S sequence: FR733675
  • Sequence Identity:
  • Total samples: 85
  • soil counts: 2
  • aquatic counts: 81
  • animal counts: 2

Safety information

risk assessment

  • @ref: 1397
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 1397
  • description: Methanohalobium evestigatum partial 16S rRNA gene, type strain DSM3721T
  • accession: FR733675
  • length: 1467
  • database: ena
  • NCBI tax ID: 2322

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanohalobium evestigatum Z-7303GCA_000196655completencbi644295
66792Methanohalobium evestigatum Z-7303, DSM 3721648028039completeimg644295
66792Methanohalobium evestigatum Z-7303644295.12plasmidpatric644295
66792Methanohalobium evestigatum Z-7303644295.10completepatric644295

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno86.239no
flagellatedno96.132no
gram-positiveno97.761no
anaerobicyes96.008yes
aerobicno94.262yes
halophileno61.227no
spore-formingno95.909no
thermophileyes58.588yes
glucose-utilyes78.895no
glucose-fermentno69.311no

External links

@ref: 1397

culture collection no.: DSM 3721, NBRC 107634, OCM 161

straininfo link

  • @ref: 76519
  • straininfo: 47750

Reference

@idauthorscataloguedoi/urltitle
1397Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3721)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3721
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76519Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47750.1StrainInfo: A central database for resolving microbial strain identifiers