Strain identifier

BacDive ID: 7011

Type strain: No

Species: Methanofollis liminatans

Strain Designation: BM1

Strain history: <- G. Zellner; BM1 (Methanoculleus sp.) <- G. Zellner {1990}

NCBI tax ID(s): 2201 (species)

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General

@ref: 3843

BacDive-ID: 7011

DSM-Number: 10196

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanofollis liminatans BM1 is an anaerobe, mesophilic archaeon that was isolated from butyrate-degrading, anaerobic fluidized-bed reactor.

NCBI tax id

  • NCBI tax id: 2201
  • Matching level: species

strain history

  • @ref: 3843
  • history: <- G. Zellner; BM1 (Methanoculleus sp.) <- G. Zellner {1990}

doi: 10.13145/bacdive7011.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanomicrobiales
  • family: Methanomicrobiaceae
  • genus: Methanofollis
  • species: Methanofollis liminatans
  • full scientific name: Methanofollis liminatans (Zellner et al. 1990) Zellner et al. 1999
  • synonyms

    • @ref: 20215
    • synonym: Methanogenium liminatans

@ref: 3843

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanomicrobiaceae

genus: Methanofollis

species: Methanofollis liminatans

full scientific name: Methanofollis liminatans (Zellner et al. 1990) Zellner et al. 1999

strain designation: BM1

type strain: no

Culture and growth conditions

culture medium

  • @ref: 3843
  • name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1523
  • composition: Name: MODIFIED METHANOBACTERIUM MEDIUM (DSMZ Medium 1523; with strain-specific modifications) Composition: Brain heart infusion 5.98802 g/l Proteose peptone 5.98802 g/l Na-acetate 3.99202 g/l NaHCO3 3.99202 g/l Yeast extract 1.99601 g/l Na-formate 1.99601 g/l Na2S x 9 H2O 0.499002 g/l KH2PO4 0.499002 g/l L-Cysteine HCl x H2O 0.499002 g/l MgSO4 x 7 H2O 0.399202 g/l NH4Cl 0.399202 g/l NaCl 0.399202 g/l CaCl2 x 2 H2O 0.0499002 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 0.000299401 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l CoCl2 x 6 H2O 0.000189621 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l ZnCl2 6.98603e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l D-(+)-biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Distilled water

culture temp

  • @ref: 3843
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3843
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 3843
  • sample type: butyrate-degrading, anaerobic fluidized-bed reactor
  • geographic location: Hannover
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2
#Engineered#Bioreactor
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_187109.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17952;96_68796;97_89438;98_121708;99_187109&stattab=map
  • Last taxonomy: Methanofollis
  • 16S sequence: Y16429
  • Sequence Identity:
  • Total samples: 89
  • soil counts: 8
  • aquatic counts: 71
  • animal counts: 1
  • plant counts: 9

Safety information

risk assessment

  • @ref: 3843
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Methanofollis liminatans 16S rRNA gene, strain DSM 10196
  • accession: Y16429
  • length: 1430
  • database: ena
  • NCBI tax ID: 2201

GC content

  • @ref: 3843
  • GC-content: 60
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 3843

culture collection no.: DSM 10196

straininfo link

  • @ref: 76472
  • straininfo: 48774

literature

  • topic: Phylogeny
  • Pubmed-ID: 10028269
  • title: Reclassification of Methanogenium tationis and Methanogenium liminatans as Methanofollis tationis gen. nov., comb. nov. and Methanofollis liminatans comb. nov. and description of a new strain of Methanofollis liminatans.
  • authors: Zellner G, Boone DR, Keswani J, Whitman WB, Woese CR, Hagelstein A, Tindall BJ, Stackebrandt E
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-49-1-247
  • year: 1999
  • mesh: Base Sequence, Euryarchaeota/*classification, Lipids/analysis, Methanomicrobiaceae/*classification, Molecular Sequence Data, Phylogeny
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3843Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10196)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10196
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76472Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48774.1StrainInfo: A central database for resolving microbial strain identifiers