Strain identifier
BacDive ID: 6982
Type strain: 
Species: Methanocaldococcus infernus
Strain Designation: ME
Strain history: DSM 11812 <-- C. Jeanthon strain ME.
NCBI tax ID(s): 573063 (strain), 67760 (species)
version 8.1 (current version)
General
@ref: 4499
BacDive-ID: 6982
DSM-Number: 11812
keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Methanocaldococcus infernus ME is an anaerobe, hyperthermophilic archaeon that was isolated from hydrothermal deep sea vent, 3000 m depth.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 573063 | strain |
| 67760 | species |
strain history
| @ref | history |
|---|---|
| 4499 | <- C. Jeanthon, ME |
| 67770 | DSM 11812 <-- C. Jeanthon strain ME. |
doi: 10.13145/bacdive6982.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanococci
- order: Methanococcales
- family: Methanocaldococcaceae
- genus: Methanocaldococcus
- species: Methanocaldococcus infernus
- full scientific name: Methanocaldococcus infernus (Jeanthon et al. 1998) Whitman 2002
synonyms
- @ref: 20215
- synonym: Methanococcus infernus
@ref: 4499
domain: Archaea
phylum: Euryarchaeota
class: Methanococci
order: Methanococcales
family: Methanocaldococcaceae
genus: Methanocaldococcus
species: Methanocaldococcus infernus
full scientific name: Methanocaldococcus infernus (Jeanthon et al. 1998) Whitman 2002
strain designation: ME
type strain: yes
Culture and growth conditions
culture medium
- @ref: 4499
- name: METHANOCALDOCOCCUS MEDIUM (DSMZ Medium 282)
- growth: yes
- link: https://mediadive.dsmz.de/medium/282
- composition: Name: METHANOCALDOCOCCUS MEDIUM (DSMZ Medium 282) Composition: MgCl2 x 6 H2O 4.05138 g/l NaHCO3 0.988142 g/l Na2S x 9 H2O 0.494071 g/l L-Cysteine HCl x H2O 0.494071 g/l KCl 0.326087 g/l NH4Cl 0.247036 g/l K2HPO4 0.13834 g/l MgSO4 x 7 H2O 0.0296443 g/l Nitrilotriacetic acid 0.0148221 g/l NaCl 0.00988142 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00988142 g/l MnSO4 x H2O 0.00494071 g/l ZnSO4 x 7 H2O 0.00177866 g/l CoSO4 x 7 H2O 0.00177866 g/l CaCl2 x 2 H2O 0.000988142 g/l FeSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l NiCl2 x 6 H2O 0.000296443 g/l AlK(SO4)2 x 12 H2O 0.000197628 g/l Na2MoO4 x 2 H2O 9.88142e-05 g/l CuSO4 x 5 H2O 9.88142e-05 g/l H3BO3 9.88142e-05 g/l Pyridoxine hydrochloride 9.88142e-05 g/l Thiamine HCl 4.94071e-05 g/l (DL)-alpha-Lipoic acid 4.94071e-05 g/l p-Aminobenzoic acid 4.94071e-05 g/l Riboflavin 4.94071e-05 g/l Nicotinic acid 4.94071e-05 g/l Calcium D-(+)-pantothenate 4.94071e-05 g/l Biotin 1.97628e-05 g/l Folic acid 1.97628e-05 g/l Na2WO4 x 2 H2O 3.95257e-06 g/l Na2SeO3 x 5 H2O 2.96443e-06 g/l Vitamin B12 9.88142e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 4499 | positive | growth | 85 | hyperthermophilic |
| 67770 | positive | growth | 85 | hyperthermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 4499
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | latitude | longitude |
|---|---|---|---|---|
| 4499 | hydrothermal deep sea vent, 3000 m depth | Mid-Atlantic Ridge, Logatchev hydrothermal vent field | 14.75 | -44.9833 |
| 67770 | Deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge at a depth of 3,000 m |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Hydrothermal vent |
| #Environmental | #Aquatic | #Marine |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_194498.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17891;96_68942;97_93145;98_126721;99_194498&stattab=map
- Last taxonomy: Methanocaldococcus
- 16S sequence: AF025822
- Sequence Identity:
- Total samples: 3
- aquatic counts: 3
Safety information
risk assessment
- @ref: 4499
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4499
- description: Methanocaldococcus infernus strain ME 16S ribosomal RNA gene, partial sequence
- accession: AF025822
- length: 1429
- database: ena
- NCBI tax ID: 67760
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanocaldococcus infernus ME | GCA_000092305 | complete | ncbi | 573063 |
| 66792 | Methanocaldococcus infernus ME | 646564547 | complete | img | 573063 |
| 66792 | Methanocaldococcus infernus ME | 573063.7 | complete | patric | 573063 |
GC content
- @ref: 4499
- GC-content: 33
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 83.969 | no |
| flagellated | no | 93.742 | no |
| gram-positive | no | 97.305 | no |
| anaerobic | yes | 99.108 | no |
| aerobic | no | 97.174 | yes |
| halophile | no | 78.009 | no |
| spore-forming | no | 96.745 | no |
| thermophile | yes | 99.999 | no |
| glucose-util | yes | 70.99 | no |
| glucose-ferment | no | 55.993 | no |
External links
@ref: 4499
culture collection no.: DSM 11812, JCM 15783
straininfo link
- @ref: 76443
- straininfo: 49184
literature
- topic: Phylogeny
- Pubmed-ID: 9734046
- title: Methanococcus infernus sp. nov., a novel hyperthermophilic lithotrophic methanogen isolated from a deep-sea hydrothermal vent.
- authors: Jeanthon C, L'Haridon S, Reysenbach AL, Vernet M, Messner P, Sleytr UB, Prieur D
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-48-3-913
- year: 1998
- mesh: Anti-Bacterial Agents/pharmacology, Base Sequence, DNA, Ribosomal/chemistry, Methanococcus/classification/growth & development/*isolation & purification, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4499 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11812) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11812 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 76443 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49184.1 | StrainInfo: A central database for resolving microbial strain identifiers |