Strain identifier

BacDive ID: 6944

Type strain: No

Species: Methanobacterium subterraneum

Strain Designation: C2BIS

Strain history: <- S. Kotelnikova, Russian Academy of Sciences, Institute of Biochemistry and Physiology of Microorganisms, Moscow, Russia; C2BIS <- S. Kotelnikova {1996}

NCBI tax ID(s): 59277 (species)

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General

@ref: 4190

BacDive-ID: 6944

DSM-Number: 11075

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobacterium subterraneum C2BIS is an anaerobe, mesophilic archaeon that was isolated from groundwater of rock tunnel at 440 m depth..

NCBI tax id

  • NCBI tax id: 59277
  • Matching level: species

strain history

  • @ref: 4190
  • history: <- S. Kotelnikova, Russian Academy of Sciences, Institute of Biochemistry and Physiology of Microorganisms, Moscow, Russia; C2BIS <- S. Kotelnikova {1996}

doi: 10.13145/bacdive6944.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanobacterium
  • species: Methanobacterium subterraneum
  • full scientific name: Methanobacterium subterraneum Kotelnikova et al. 1998

@ref: 4190

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobacterium

species: Methanobacterium subterraneum

full scientific name: Methanobacterium subterraneum Kotelnikova et al. 1998

strain designation: C2BIS

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4190METHANOBACTERIUM SUBTERRANEUM MEDIUM (DSMZ Medium 814)yeshttps://mediadive.dsmz.de/medium/814Name: METHANOBACTERIUM SUBTERRANEUM MEDIUM (DSMZ Medium 814) Composition: Na-formate 3.2 g/l Tryptone 1.0 g/l Na-acetate 1.0 g/l Na2CO3 1.0 g/l K2HPO4 0.5 g/l NH4Cl 0.4 g/l L-Cysteine HCl x H2O 0.25 g/l Na2S x 9 H2O 0.25 g/l MgCl2 x 6 H2O 0.03 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l ZnSO4 x 7 H2O 0.0018 g/l CoSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l Pyridoxine hydrochloride 0.0003 g/l Thiamine-HCl x 2 H2O 0.0002 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l Nicotinic acid 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l Vitamin B12 0.0001 g/l Calcium pantothenate 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l p-Aminobenzoic acid 8e-05 g/l D-(+)-biotin 2e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water
4190METHANOBACTERIUM MEDIUM (DSMZ Medium 119)yeshttps://mediadive.dsmz.de/medium/119Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

culture temp

  • @ref: 4190
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4190
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 4190
  • sample type: groundwater of rock tunnel at 440 m depth.
  • geographic location: Äspö Hard Rock Tunnel Laboratory
  • country: Sweden
  • origin.country: SWE
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Groundwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_188779.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17808;96_68789;97_90296;98_122724;99_188779&stattab=map
  • Last taxonomy: Methanobacterium subterraneum subclade
  • 16S sequence: X99045
  • Sequence Identity:
  • Total samples: 287
  • soil counts: 32
  • aquatic counts: 222
  • animal counts: 13
  • plant counts: 20

Safety information

risk assessment

  • @ref: 4190
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4190
  • description: Methanobacterium subterraneum 16S rRNA gene, strain C2BIS
  • accession: X99045
  • length: 1430
  • database: ena
  • NCBI tax ID: 59277

External links

@ref: 4190

culture collection no.: DSM 11075

straininfo link

  • @ref: 76406
  • straininfo: 49021

Reference

@idauthorscataloguedoi/urltitle
4190Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11075)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11075
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76406Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49021.1StrainInfo: A central database for resolving microbial strain identifiers