Strain identifier
BacDive ID: 6891
Type strain: 
Species: Methanothermobacter thermautotrophicus
Strain Designation: SF-4
Strain history: <- S. Oi, SF-4 (Methanobacterium thermoformicicum)
NCBI tax ID(s): 145262 (species)
version 8.1 (current version)
General
@ref: 2680
BacDive-ID: 6891
DSM-Number: 6457
keywords: Archaea, anaerobe, thermophilic
description: Methanothermobacter thermautotrophicus SF-4 is an anaerobe, thermophilic archaeon that was isolated from thermophilic methane sludge.
NCBI tax id
- NCBI tax id: 145262
- Matching level: species
strain history
- @ref: 2680
- history: <- S. Oi, SF-4 (Methanobacterium thermoformicicum)
doi: 10.13145/bacdive6891.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanothermobacter
- species: Methanothermobacter thermautotrophicus
- full scientific name: Methanothermobacter thermautotrophicus corrig. (Zeikus and Wolfe 1972) Wasserfallen et al. 2000
synonyms
@ref synonym 20215 Methanobacterium thermautotrophicum 20215 Methanobacterium thermalcaliphilum 20215 Methanobacterium thermoautotrophicus 20215 Methanobacterium thermoalcaliphilum 20215 Methanobacterium thermoformicicum 20215 Methanobacterium thermoautotrophicum
@ref: 2680
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanothermobacter
species: Methanothermobacter thermautotrophicus
full scientific name: Methanothermobacter thermautotrophicus (Zeikus and Wolfe 1972) Wasserfallen et al. 2000
strain designation: SF-4
type strain: no
Culture and growth conditions
culture medium
- @ref: 2680
- name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119
- composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
culture temp
- @ref: 2680
- growth: positive
- type: growth
- temperature: 60
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 2680
- oxygen tolerance: anaerobe
compound production
- @ref: 2680
- compound: aspartate aminotransferase
Isolation, sampling and environmental information
isolation
- @ref: 2680
- sample type: thermophilic methane sludge
- country: Thailand
- origin.country: THA
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Mud (Sludge) |
| #Condition | #Anoxic (anaerobic) | |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 2680
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
GC content
- @ref: 2680
- GC-content: 50.7
External links
@ref: 2680
culture collection no.: DSM 6457
straininfo link
- @ref: 76355
- straininfo: 48203
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 1295891 | Further studies on aspartate aminotransferase of thermophilic methanogens by analysis of general properties, bound cofactors, and subunit structures. | Tanaka T, Yamamoto S, Taniguchi M, Hayashi H, Kuramitsu S, Kagamiyama H, Oi S | J Biochem | 10.1093/oxfordjournals.jbchem.a123981 | 1992 | Aspartate Aminotransferases/chemistry/isolation & purification/*metabolism, Chromatography, DEAE-Cellulose, Chromatography, Gel, Chromatography, Ion Exchange, Hot Temperature, Kinetics, Macromolecular Substances, Methanobacterium/*enzymology, Molecular Weight, Species Specificity, Spectrophotometry | Phylogeny |
| Enzymology | 8206881 | Aspartate aminotransferase from a thermophilic formate-utilizing methanogen, Methanobacterium thermoformicicum strain SF-4: relation to serine and phosphoserine aminotransferases, but not to the aspartate aminotransferase family. | Tanaka T, Yamamoto S, Moriya T, Taniguchi M, Hayashi H, Kagamiyama H, Oi S | J Biochem | 10.1093/oxfordjournals.jbchem.a124334 | 1994 | Amino Acid Sequence, Animals, Aspartate Aminotransferases/*chemistry/genetics/metabolism, Base Composition, Base Sequence, Blotting, Southern, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Gene Expression/genetics, Methanobacterium/*enzymology, Molecular Sequence Data, Nucleic Acid Hybridization, Rats, Restriction Mapping, Sequence Homology, Amino Acid, Transaminases/*chemistry/genetics/metabolism | Metabolism |
| Enzymology | 8282731 | Potassium-stimulating mechanism of geranylgeranyl diphosphate synthase of Methanobacterium thermoformicicum SF-4. | Tachibana A, Tanaka T, Taniguchi M, Oi S | J Biochem | 10.1093/oxfordjournals.jbchem.a124186 | 1993 | *Alkyl and Aryl Transferases, Cations, Monovalent/pharmacology, Farnesyltranstransferase, Kinetics, Methanobacterium/*enzymology, Potassium/*pharmacology, Transferases/antagonists & inhibitors/*drug effects | Pathogenicity |
| Enzymology | 27280996 | Purification and Characterization of Geranylgeranyl Diphosphate Synthase from Methanobacterium thermoformicicum SF-4. | Tachibana A, Tanaka T, Taniguchi M, Susumu O | Biosci Biotechnol Biochem | 10.1271/bbb.57.1129 | 1993 |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 2680 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6457) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6457 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 76355 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48203.1 | StrainInfo: A central database for resolving microbial strain identifiers |