Strain identifier

BacDive ID: 6853

Type strain: Yes

Species: Weissella soli

Strain Designation: Mi268, Mi 268

Strain history: CIP <- 2002, S. Roos <- J. Magnusson, Uppsala, Sweden: strain Mi 268

NCBI tax ID(s): 155866 (species)

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General

@ref: 5344

BacDive-ID: 6853

DSM-Number: 14420

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, psychrophilic, Gram-positive, rod-shaped

description: Weissella soli Mi268 is an anaerobe, psychrophilic, Gram-positive bacterium that was isolated from garden soil.

NCBI tax id

  • NCBI tax id: 155866
  • Matching level: species

strain history

@refhistory
5344<- S. Roos <- J. Magnusson; Mi268
67770LMG 20113 <-- S. Roos Mi 268 <-- J. Magnusson.
122671CIP <- 2002, S. Roos <- J. Magnusson, Uppsala, Sweden: strain Mi 268

doi: 10.13145/bacdive6853.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Weissella
  • species: Weissella soli
  • full scientific name: Weissella soli Magnusson et al. 2002

@ref: 5344

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Weissella

species: Weissella soli

full scientific name: Weissella soli Magnusson et al. 2002

strain designation: Mi268, Mi 268

type strain: yes

Morphology

cell morphology

  • @ref: 122671
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5344MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38216MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122671CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5344positivegrowth20psychrophilic
38216positivegrowth30mesophilic
57286positivegrowth37mesophilic
67770positivegrowth30mesophilic
122671positivegrowth22-45
122671nogrowth10psychrophilic
122671nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57286anaerobe
57286microaerophile
122671facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122671nitrate-reduction17632
122671nitrite-reduction16301
122671nitrate+respiration17632

antibiotic resistance

  • @ref: 122671
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite tests

  • @ref: 122671
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
122671oxidase-
122671alcohol dehydrogenase-1.1.1.1
122671catalase-1.11.1.6
122671lysine decarboxylase-4.1.1.18
122671ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122671-----------+---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122671---+++----+-+/---------+/---++-+-+++--+---------+--+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
5344garden soilSwedenSWEEurope
57286Garden soilSwedenSWEEuropeUppsala
67770Garden soil
122671Environment, Garden soilSwedenSWEEuropeUppsala

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Engineered#Agriculture#Garden

taxonmaps

  • @ref: 69479
  • File name: preview.99_12599.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_155;96_1590;97_7727;98_9498;99_12599&stattab=map
  • Last taxonomy: Weissella soli subclade
  • 16S sequence: LC258131
  • Sequence Identity:
  • Total samples: 2102
  • soil counts: 112
  • aquatic counts: 238
  • animal counts: 1582
  • plant counts: 170

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53441Risk group (German classification)
1226711Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67770Weissella soli 16S ribosomal RNA gene, complete sequenceAY0282601558ena155866
67770Weissella soli gene for 16S ribosomal RNA, partial sequence, strain: JCM 12536LC2581311518ena155866

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Weissella soli NBRC 106074GCA_007991555contigncbi155866
66792Weissella soli strain CCUG 46608155866.9wgspatric155866
66792Weissella soli strain NBRC 106074155866.8wgspatric155866
67770Weissella soli CCUG 46608GCA_012396465contigncbi155866

GC content

  • @ref: 5344
  • GC-content: 43
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno95.813no
gram-positiveyes96.971no
anaerobicno85.389no
aerobicno97.505no
halophileyes91.538no
spore-formingno95.255no
thermophileno99.349yes
glucose-utilyes91.676no
flagellatedno97.801no
glucose-fermentyes88.294no

External links

@ref: 5344

culture collection no.: DSM 14420, LMG 20113, CCUG 46608, JCM 12536, KCTC 3789, NBRC 106074, CIP 107584

straininfo link

  • @ref: 76318
  • straininfo: 88371

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12054246Weissella soli sp. nov., a lactic acid bacterium isolated from soil.Magnusson J, Jonsson H, Schnurer J, Roos SInt J Syst Evol Microbiol10.1099/00207713-52-3-8312002Bacterial Proteins/analysis, DNA, Ribosomal/analysis, Gram-Positive Rods/chemistry/*classification/isolation & purification/metabolism, Lactic Acid/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyMetabolism
Phylogeny19060047Weissella ghanensis sp. nov., isolated from a Ghanaian cocoa fermentation.De Bruyne K, Camu N, Lefebvre K, De Vuyst L, Vandamme PInt J Syst Evol Microbiol10.1099/ijs.0.65853-02008Cacao/*metabolism/*microbiology, *Fermentation, Ghana, Lactobacillaceae/*classification/genetics/isolation & purification/*physiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Species SpecificityGenetics
Phylogeny22843715Weissella oryzae sp. nov., isolated from fermented rice grains.Tohno M, Kitahara M, Inoue H, Uegaki R, Irisawa T, Ohkuma M, Tajima KInt J Syst Evol Microbiol10.1099/ijs.0.043612-02012Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Fermentation, Molecular Sequence Data, Oryza/*microbiology, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Weissella/*classification/genetics/isolation & purificationGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5344Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14420)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14420
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38216Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5035
57286Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46608)https://www.ccug.se/strain?id=46608
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76318Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID88371.1StrainInfo: A central database for resolving microbial strain identifiers
122671Curators of the CIPCollection of Institut Pasteur (CIP 107584)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107584