Strain identifier

BacDive ID: 6752

Type strain: Yes

Species: Legionella cherrii

Strain Designation: ORW

Strain history: CIP <- 1993, R.F. Benson, CDC: strain ORW <- R.L. Tyndall

NCBI tax ID(s): 1122160 (strain), 28084 (species)

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General

@ref: 7971

BacDive-ID: 6752

DSM-Number: 19213

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, human pathogen

description: Legionella cherrii ORW is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from thermally altered water.

NCBI tax id

NCBI tax idMatching level
1122160strain
28084species

strain history

@refhistory
7971<- H. E. Müller <- ATCC <- W. F. Bibb <- R. L. Tyndall
118230CIP <- 1993, R.F. Benson, CDC: strain ORW <- R.L. Tyndall

doi: 10.13145/bacdive6752.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella cherrii
  • full scientific name: Legionella cherrii Brenner et al. 1985

@ref: 7971

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella cherrii

full scientific name: Legionella cherrii Brenner et al. 1985

strain designation: ORW

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.994
118230rod-shapedyes

colony morphology

  • @ref: 7971
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7971BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40808MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
118230CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
7971positivegrowth37mesophilic
40808positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 7971
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.979

enzymes

@refvalueactivityec
7971catalase-1.11.1.6
7971cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
118230oxidase-
118230catalase+1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118230-+++-++---++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
7971thermally altered waterMinnesotaUSAUSANorth America
49702Water1982
118230Environment, Thermally altered waterMinnesotaUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_85188.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_35507;97_45086;98_59003;99_85188&stattab=map
  • Last taxonomy: Legionellaceae
  • 16S sequence: Z49720
  • Sequence Identity:
  • Total samples: 613
  • soil counts: 193
  • aquatic counts: 235
  • animal counts: 156
  • plant counts: 29

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
7971yes, in single cases1Risk group (German classification)
1182302Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella cherrii strain MDC1584 16S ribosomal RNA gene, partial sequenceJF720381524ena28084
20218L.cherrii (ATCC 35252) gene for 16S rRNAX734041467ena28084
20218L.cherrii gene for ribosomal RNA, small subunitZ497201368ena28084
7971XJ3404ena

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella cherrii DSM 19213GCA_000621385scaffoldncbi1122160
66792Legionella cherrii NCTC11976GCA_900635815completencbi28084
66792Legionella cherrii ORWGCA_900640085scaffoldncbi28084
66792Legionella cherrii ORWGCA_001467035scaffoldncbi28084
66792Legionella cherrii DSM 192131122160.4wgspatric1122160
66792Legionella cherrii strain NCTC1197628084.6completepatric28084
66792Legionella cherrii strain ORW28084.5wgspatric28084
66792Legionella cherrii DSM 192132558309037draftimg1122160
66792Legionella cherrii ORW2667527672draftimg28084

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno70.058no
gram-positiveno98.447no
anaerobicno98.057yes
aerobicno88.222no
halophileno88.142no
spore-formingno93.709no
thermophileno99.277yes
glucose-utilyes67.194no
motileyes88.834no
glucose-fermentno86.849no

External links

@ref: 7971

culture collection no.: DSM 19213, ATCC 35252, CCUG 29666, CIP 103842, NCTC 11976

straininfo link

  • @ref: 76222
  • straininfo: 40947

literature

  • Pubmed-ID: 28352254
  • title: Distribution of Secretion Systems in the Genus Legionella and Its Correlation with Pathogenicity.
  • authors: Qin T, Zhou H, Ren H, Liu W
  • journal: Front Microbiol
  • DOI: 10.3389/fmicb.2017.00388
  • year: 2017

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7971Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19213)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19213
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40808Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15684
49702Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 29666)https://www.ccug.se/strain?id=29666
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76222Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40947.1StrainInfo: A central database for resolving microbial strain identifiers
118230Curators of the CIPCollection of Institut Pasteur (CIP 103842)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103842