Strain identifier
BacDive ID: 6750
Type strain: 
Species: Legionella spiritensis
Strain Designation: MSH-9
Strain history: <- CCUG <- NCTC <- ATCC <- W. F. Bibb <- J. Campbell; MSH-9
NCBI tax ID(s): 452 (species)
version 8.1 (current version)
General
@ref: 8039
BacDive-ID: 6750
DSM-Number: 19324
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic
description: Legionella spiritensis MSH-9 is a microaerophile, mesophilic bacterium that was isolated from lake water.
NCBI tax id
- NCBI tax id: 452
- Matching level: species
strain history
- @ref: 8039
- history: <- CCUG <- NCTC <- ATCC <- W. F. Bibb <- J. Campbell; MSH-9
doi: 10.13145/bacdive6750.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Legionellales
- family: Legionellaceae
- genus: Legionella
- species: Legionella spiritensis
- full scientific name: Legionella spiritensis Brenner et al. 1985
@ref: 8039
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella spiritensis
full scientific name: Legionella spiritensis Brenner et al. 1985
strain designation: MSH-9
type strain: yes
Morphology
colony morphology
- @ref: 8039
- incubation period: 1-2 days
Culture and growth conditions
culture medium
- @ref: 8039
- name: BCYE-AGAR (DSMZ Medium 585)
- growth: yes
- link: https://mediadive.dsmz.de/medium/585
- composition: Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
culture temp
- @ref: 8039
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 8039
- oxygen tolerance: microaerophile
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68377 | 15824 | D-fructose | - | builds acid from |
| 68377 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68377 | tryptophan deaminase | - | 4.1.99.1 |
| 68377 | lipase | + |
API NH
| @ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 8039 | +/- | - | - | +/- | + | - | + | + | - | + | + | - | - |
| 8039 | +/- | - | - | - | + | - | + | + | - | - | + | - | - |
| 8039 | - | +/- | - | - | +/- | + | - | + | + | - | + | + | - |
| 8039 | - | +/- | - | - | - | + | - | + | + | - | - | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 8039
- sample type: lake water
- geographic location: Spirit Lake, Mt St. Helens
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Lake (large)
taxonmaps
- @ref: 69479
- File name: preview.99_2612.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_1395;97_1663;98_2022;99_2612&stattab=map
- Last taxonomy: Legionella spiritensis subclade
- 16S sequence: HF558375
- Sequence Identity:
- Total samples: 897
- soil counts: 385
- aquatic counts: 386
- animal counts: 97
- plant counts: 29
Safety information
risk assessment
- @ref: 8039
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Legionella spiritensis partial 16S rRNA gene, strain Bibb HSH-9 | HF558375 | 1516 | ena | 452 |
| 8039 | Legionella spiritensis gene for 16S ribosomal RNA, partial sequence | AB211171 | 474 | ena | 452 |
Genome sequences
- @ref: 66792
- description: Legionella spiritensis strain Mt.St.Helens-9
- accession: 452.5
- assembly level: wgs
- database: patric
- NCBI tax ID: 452
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| gram-positive | no | 98.268 | no |
| anaerobic | no | 98.717 | yes |
| halophile | no | 77.659 | no |
| spore-forming | no | 95.098 | no |
| glucose-util | yes | 72.78 | no |
| aerobic | no | 80.996 | no |
| flagellated | no | 76.164 | no |
| motile | yes | 80.933 | no |
| thermophile | no | 98.026 | yes |
| glucose-ferment | no | 85.666 | no |
External links
@ref: 8039
culture collection no.: DSM 19324, ATCC 35249, CCUG 31118, JCM 7562
straininfo link
- @ref: 76220
- straininfo: 92971
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 8039 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19324) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19324 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 68377 | Automatically annotated from API NH | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 76220 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID92971.1 | StrainInfo: A central database for resolving microbial strain identifiers |