Archive BacDiveID:10.13145/bacdive6749.20230509.8.1

Strain identifier

BacDive ID: 6749

Type strain:

Species: Legionella hackeliae

Strain Designation: R 44, 798-PA-H

Strain history: CIP <- 1997, NCTC <- ATCC <- H.W. Wilkinson: strain 798-PA-H

NCBI tax ID(s): 449 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 17999

BacDive-ID: 6749

DSM-Number: 25323

keywords: genome sequence, Bacteria, microaerophile, mesophilic, motile

description: Legionella hackeliae R 44 is a microaerophile, mesophilic, motile bacterium that was isolated from human lung aspirate.

NCBI tax id

NCBI tax id: 449
Matching level: species

strain history

@ref	history
17999	<- H. E. Müller <- ATCC <- L. Thacker <- E. J. Wing; 798-PA-H
119760	CIP <- 1997, NCTC <- ATCC <- H.W. Wilkinson: strain 798-PA-H

doi: 10.13145/bacdive6749.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella hackeliae
full scientific name: Legionella hackeliae Brenner et al. 1985

@ref: 17999

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella hackeliae

full scientific name: Legionella hackeliae Brenner et al. 1985

strain designation: R 44, 798-PA-H

type strain: no

Morphology

cell morphology

@ref	motility	confidence	gram stain	cell shape
69480	yes	96.538
69480		99.999	negative
119760	yes		negative	rod-shaped

colony morphology

@ref: 17999
incubation period: 1-2 days

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
17999	BCYE-AGAR (DSMZ Medium 585)	yes	https://mediadive.dsmz.de/medium/585	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
36267	MEDIUM 23 - for Afipia and Legionella	yes		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119760	CIP Medium 23	yes	https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@ref	growth	type	temperature	range
17999	positive	growth	37	mesophilic
36267	positive	growth	37	mesophilic

Physiology and metabolism

oxygen tolerance

@ref: 17999
oxygen tolerance: microaerophile

spore formation

@ref	spore formation	confidence
69481	no	100
69480	no	99.992

enzymes

@ref	value	activity	ec
17999	catalase	+	1.11.1.6
17999	cytochrome-c oxidase	+	1.9.3.1

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
17999	human lung aspirate	Pennsylvania	USA	USA	North America
119760	Human, Lung aspirate

isolation source categories

Cat1	Cat2	Cat3
#Host	#Human
#Host Body-Site	#Oral cavity and airways	#Lung
#Host Body Product	#Fluids	#Aspirate

Safety information

risk assessment

@ref	biosafety level	biosafety level comment
17999	2	Risk group (German classification)
119760	2	Risk group (French classification)

Sequence information

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Legionella hackeliae 798-PA-H	GCA_001467705	scaffold	ncbi	449
66792	Legionella hackeliae strain 798-PA-H	449.7	wgs	patric	449
66792	Legionella hackeliae 798-PA-H	2671180995	draft	img	449

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	no	100	no
flagellated	no	79.956	no
gram-positive	no	98.186	no
anaerobic	no	98.185	yes
aerobic	no	84.679	yes
halophile	no	91.49	no
spore-forming	no	92.698	no
glucose-util	yes	71.539	no
motile	yes	80.28	no
thermophile	no	98.871	yes
glucose-ferment	no	85.058	no

External links

@ref: 17999

culture collection no.: DSM 25323, ATCC 35999, NCTC 11980, CIP 105112

straininfo link

@ref: 76219
straininfo: 46022

literature

topic: Phylogeny
Pubmed-ID: 4077960
title: Second serogroup of Legionella hackeliae isolated from a patient with pneumonia.
authors: Wilkinson HW, Thacker WL, Steigerwalt AG, Brenner DJ, Ampel NM, Wing EJ
journal: J Clin Microbiol
DOI: 10.1128/jcm.22.4.488-489.1985
year: 1985
mesh: Agglutination Tests, DNA, Bacterial/analysis, Female, Humans, Legionella/classification/*isolation & purification/physiology, Legionnaires' Disease/*microbiology, Middle Aged, Nucleic Acid Hybridization, Pneumonia/*microbiology, Serotyping
topic2: Pathogenicity

Reference

@id	authors	catalogue	doi/url	title
17999	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25323)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-25323
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36267	Curators of the CIP		https://brclims.pasteur.fr/brcWeb/souche/detail/1/17096
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
76219	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID46022.1	StrainInfo: A central database for resolving microbial strain identifiers
119760	Curators of the CIP	Collection of Institut Pasteur (CIP 105112)	https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105112