Strain identifier

BacDive ID: 6730

Type strain: No

Species: Legionella longbeachae

Strain Designation: R21, Tucker 1

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.F. Bibb: strain Tucker 1

NCBI tax ID(s): 450 (species)

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General

@ref: 17994

BacDive-ID: 6730

DSM-Number: 25315

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Legionella longbeachae R21 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human lung.

NCBI tax id

  • NCBI tax id: 450
  • Matching level: species

strain history

@refhistory
17994<- H. E. Müller <- ATCC <- W. F. Bibb; Tucker 1
120648CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.F. Bibb: strain Tucker 1

doi: 10.13145/bacdive6730.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella longbeachae
  • full scientific name: Legionella longbeachae McKinney et al. 1982

@ref: 17994

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella longbeachae

full scientific name: Legionella longbeachae McKinney et al. 1982

strain designation: R21, Tucker 1

type strain: no

Morphology

cell morphology

  • @ref: 120648
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 17994
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17994BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
35589MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
120648CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
17994positivegrowth37mesophilic
35589positivegrowth37mesophilic
57291positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17994microaerophile
57291microaerophile
120648obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12064817632nitrate-reduction
12064816301nitrite-reduction

metabolite production

  • @ref: 120648
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
17994catalase+1.11.1.6
17994cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120648oxidase-
120648alcohol dehydrogenase-1.1.1.1
120648catalase+1.11.1.6
120648lysine decarboxylase-4.1.1.18
120648ornithine decarboxylase-4.1.1.17
120648urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    57291C14:00.314
    57291C15:00.515
    57291C16:03.216
    57291C17:00.617
    57291C14:0 ISO9.913.618
    57291C14:1 ω5c0.613.899
    57291C15:0 ANTEISO18.614.711
    57291C15:0 ISO0.514.621
    57291C15:1 ω6c314.856
    57291C16:0 iso23.315.626
    57291C16:0 iso 3OH0.517.145
    57291C16:1 ω5c0.815.908
    57291C16:1 ω7c27.815.819
    57291C17:0 anteiso4.616.722
    57291C17:0 CYCLO2.516.888
    57291C17:0 iso0.716.629
    57291C18:1 ω7c /12t/9t2.117.824
    57291Unidentified0.3
    57291Unidentified0.4
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120648-+++-+----++--------

Isolation, sampling and environmental information

isolation

  • @ref: 17994
  • sample type: human lung
  • geographic location: Tucker, GA
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Lung

taxonmaps

  • @ref: 69479
  • File name: preview.99_845.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_584;98_671;99_845&stattab=map
  • Last taxonomy: Legionella
  • 16S sequence: FN650140
  • Sequence Identity:
  • Total samples: 1856
  • soil counts: 787
  • aquatic counts: 754
  • animal counts: 213
  • plant counts: 102

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
179942Risk group (German classification)
1206482Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella longbeachae strain ATCC 33484 16S ribosomal RNA gene, partial sequenceAY4447411470ena450
17994Legionella longbeachae NSW150, complete genomeFN6501404077332ena661367

External links

@ref: 17994

culture collection no.: DSM 25315, ATCC 33484, CCUG 46623, CIP 103881, NCTC 11530

straininfo link

  • @ref: 76200
  • straininfo: 44959

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1980403Genetic relatedness of Legionella longbeachae isolates from human and environmental sources in Australia.Lanser JA, Adams M, Doyle R, Sangster N, Steele TWAppl Environ Microbiol10.1128/aem.56.9.2784-2790.19901990Australia, Bacterial Typing Techniques, DNA Probes, Genetic Markers, Humans, Isoenzymes/genetics, Legionella/enzymology/genetics/*isolation & purification, Polymorphism, Restriction Fragment Length, SerotypingPathogenicity
Phylogeny7037837Recognition of a second serogroup of Legionella longbeachae.Bibb WF, Sorg RJ, Thomason BM, Hicklin MD, Steigerwalt AG, Brenner DJ, Wulf MRJ Clin Microbiol10.1128/jcm.14.6.674-677.19811981Antigens, Bacterial/*genetics, Fluorescent Antibody Technique, Legionella/*immunology/isolation & purification, Legionnaires' Disease/microbiology, Lung/microbiology, Phenotype, Serology, Species SpecificityPhenotype
Genetics9529072Sequence analysis of the mip gene of the soilborne pathogen Legionella longbeachae.Doyle RM, Steele TW, McLennan AM, Parkinson IH, Manning PA, Heuzenroeder MWInfect Immun10.1128/IAI.66.4.1492-1499.19981998Amino Acid Sequence, Animals, Bacterial Proteins/chemistry/*genetics/physiology, Base Sequence, *Genes, Bacterial, Genetic Complementation Test, Guinea Pigs, *Immunophilins, Legionella/*genetics/pathogenicity, Membrane Proteins/chemistry/*genetics/physiology, Molecular Sequence Data, *Peptidylprolyl Isomerase, Rabbits, *Soil Microbiology, Transcription, Genetic, VirulenceTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17994Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25315)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25315
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35589Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15727
57291Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46623)https://www.ccug.se/strain?id=46623
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76200Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44959.1StrainInfo: A central database for resolving microbial strain identifiers
120648Curators of the CIPCollection of Institut Pasteur (CIP 103881)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103881