Strain identifier

BacDive ID: 673

Type strain: No

Species: Cytobacillus firmus

Strain Designation: 854, a Hambourg

Strain history: CIP <- 1952, R.E. Gordon, New Jersey Agri. Exper. Stat., New Brunswick, USA: strain 854 <- 1937 J.R. Porter <- G. Bredemann: strain a Hambourg

NCBI tax ID(s): 1399 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.3 (current version):
version 9.2:
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.3 (current version)

General

@ref: 149

BacDive-ID: 673

DSM-Number: 359

keywords: 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Cytobacillus firmus 854 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Bacillaceae.

NCBI tax id

  • NCBI tax id: 1399
  • Matching level: species

strain history

@refhistory
149<- CCM <- NCIB <- ATCC <- N.R. Smith, 854 <- G. Bredemann, strain A
67770IAM 1188 <-- IFO 3330 <-- ATCC 8247 <-- N. R. Smith NRS 854 <-- J. R. Porter <-- G. Bredemann strain A.
119355CIP <- 1952, R.E. Gordon, New Jersey Agri. Exper. Stat., New Brunswick, USA: strain 854 <- 1937 J.R. Porter <- G. Bredemann: strain a Hambourg

doi: 10.13145/bacdive673.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Cytobacillus
  • species: Cytobacillus firmus
  • full scientific name: Cytobacillus firmus (Bredemann and Werner 1933) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus firmus

@ref: 149

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Cytobacillus

species: Cytobacillus firmus

full scientific name: Cytobacillus firmus (Bredemann and Werner 1933) Patel and Gupta 2020

strain designation: 854, a Hambourg

type strain: no

Morphology

cell morphology

  • @ref: 119355
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 119355
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35265MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
149NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
119355CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
149positivegrowth30
35265positivegrowth30
67770positivegrowth30
119355positivegrowth10-37
119355nogrowth45
119355nogrowth55

culture pH

  • @ref: 119355
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 119355
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 119355
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
119355NaClpositivegrowth0-6 %
119355NaClnogrowth8 %
119355NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11935516947citrate-carbon source
1193554853esculin-hydrolysis
119355606565hippurate-hydrolysis
11935517632nitrate+reduction
11935516301nitrite-reduction
11935517632nitrate-respiration
68371Potassium 5-ketogluconate-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837117754glycerol-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from

metabolite production

  • @ref: 119355
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11935515688acetoin-
11935517234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382lipase (C 14)-
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382alkaline phosphatase-3.1.3.1
119355oxidase-
119355beta-galactosidase+3.2.1.23
119355alcohol dehydrogenase-1.1.1.1
119355gelatinase+
119355amylase-
119355DNase-
119355caseinase+3.4.21.50
119355catalase+1.11.1.6
119355tween esterase-
119355gamma-glutamyltransferase-2.3.2.2
119355lecithinase-
119355lipase-
119355lysine decarboxylase-4.1.1.18
119355ornithine decarboxylase-4.1.1.17
119355urease-3.5.1.5
68382acid phosphatase-3.1.3.2

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119355--++-------+---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119355-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119355+--+---+-++------------------+---+---------------+---------+---------------+---++-----+---+++++---+

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
1491Risk group (German classification)
1193551Risk group (French classification)

Sequence information

16S sequences

  • @ref: 124043
  • description: Bacillus firmus gene for 16S rRNA, partial sequence, strain: NBRC 3330.
  • accession: AB680064
  • length: 1477
  • database: nuccore
  • NCBI tax ID: 1399

External links

@ref: 149

culture collection no.: DSM 359, ATCC 8247, CCM 2212, NCIB 8162, JCM 20092, IAM 1188, IFO 3330, NBRC 3330, OUT 8399, RIMD 0209001, CIP 52.69, NCIMB 8162

straininfo link

  • @ref: 70348
  • straininfo: 13924

Reference

@idauthorscataloguedoi/urltitle
149Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 359)https://www.dsmz.de/collection/catalogue/details/culture/DSM-359
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35265Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/13312
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
70348Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13924.1StrainInfo: A central database for resolving microbial strain identifiers
119355Curators of the CIPCollection of Institut Pasteur (CIP 52.69)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2052.69
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy