Strain identifier

BacDive ID: 673

Type strain: No

Species: Cytobacillus firmus

Strain Designation: 854, a Hambourg

Strain history: CIP <- 1952, R.E. Gordon, New Jersey Agri. Exper. Stat., New Brunswick, USA: strain 854 <- 1937 J.R. Porter <- G. Bredemann: strain a Hambourg

NCBI tax ID(s): 1399 (species)

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General

@ref: 149

BacDive-ID: 673

DSM-Number: 359

keywords: Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Cytobacillus firmus 854 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Bacillaceae.

NCBI tax id

  • NCBI tax id: 1399
  • Matching level: species

strain history

@refhistory
149<- CCM <- NCIB <- ATCC <- N.R. Smith, 854 <- G. Bredemann, strain A
67770IAM 1188 <-- IFO 3330 <-- ATCC 8247 <-- N. R. Smith NRS 854 <-- J. R. Porter <-- G. Bredemann strain A.
119355CIP <- 1952, R.E. Gordon, New Jersey Agri. Exper. Stat., New Brunswick, USA: strain 854 <- 1937 J.R. Porter <- G. Bredemann: strain a Hambourg

doi: 10.13145/bacdive673.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Cytobacillus
  • species: Cytobacillus firmus
  • full scientific name: Cytobacillus firmus (Bredemann and Werner 1933) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus firmus

@ref: 149

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Cytobacillus

species: Cytobacillus firmus

full scientific name: Cytobacillus firmus (Bredemann and Werner 1933) Patel and Gupta 2020

strain designation: 854, a Hambourg

type strain: no

Morphology

cell morphology

  • @ref: 119355
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 119355
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35265MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
149NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
119355CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
149positivegrowth30mesophilic
35265positivegrowth30mesophilic
67770positivegrowth30mesophilic
119355positivegrowth10-37
119355nogrowth45thermophilic
119355nogrowth55thermophilic

culture pH

  • @ref: 119355
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 119355
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 119355
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
119355NaClpositivegrowth0-6 %
119355NaClnogrowth8 %
119355NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119355citrate-carbon source16947
119355esculin-hydrolysis4853
119355hippurate-hydrolysis606565
119355nitrate+reduction17632
119355nitrite-reduction16301
119355nitrate-respiration17632

metabolite production

  • @ref: 119355
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11935515688acetoin-
11935517234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119355oxidase-
119355beta-galactosidase+3.2.1.23
119355alcohol dehydrogenase-1.1.1.1
119355gelatinase+
119355amylase-
119355DNase-
119355caseinase+3.4.21.50
119355catalase+1.11.1.6
119355tween esterase-
119355gamma-glutamyltransferase-2.3.2.2
119355lecithinase-
119355lipase-
119355lysine decarboxylase-4.1.1.18
119355ornithine decarboxylase-4.1.1.17
119355urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119355--++-------+---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119355-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119355+--+---+-++------------------+---+---------------+---------+---------------+---++-----+---+++++---+

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
1491Risk group (German classification)
1193551Risk group (French classification)

External links

@ref: 149

culture collection no.: DSM 359, ATCC 8247, CCM 2212, NCIB 8162, JCM 20092, IAM 1188, IFO 3330, NBRC 3330, OUT 8399, RIMD 0209001, CIP 52.69, NCIMB 8162

straininfo link

  • @ref: 70348
  • straininfo: 13924

Reference

@idauthorscataloguedoi/urltitle
149Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 359)https://www.dsmz.de/collection/catalogue/details/culture/DSM-359
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35265Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/13312
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
70348Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13924.1StrainInfo: A central database for resolving microbial strain identifiers
119355Curators of the CIPCollection of Institut Pasteur (CIP 52.69)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2052.69