Strain identifier

BacDive ID: 6723

Type strain: No

Species: Legionella pneumophila subsp. pneumophila

Strain Designation: R67, Thacker 570-CO-H, 570-CO-H SG 12, 570-CO-H

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.L. Thacker: strain 570-CO-H <- L.B. Reller

NCBI tax ID(s): 933093 (strain), 91891 (subspecies)

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General

@ref: 17945

BacDive-ID: 6723

DSM-Number: 25224

keywords: genome sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella pneumophila subsp. pneumophila R67 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human lung tissue.

NCBI tax id

NCBI tax idMatching level
933093strain
91891subspecies

strain history

@refhistory
17945<- H. E. Müller; 570-CO-H SG 12 <- ATCC <- W. L. Thacker <- L. B. Reller
121907CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.L. Thacker: strain 570-CO-H <- L.B. Reller

doi: 10.13145/bacdive6723.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella pneumophila subsp. pneumophila
  • full scientific name: Legionella pneumophila subsp. pneumophila (Brenner et al. 1979) Brenner et al. 1989

@ref: 17945

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella pneumophila subsp. pneumophila

full scientific name: Legionella pneumophila subsp. pneumophila (Brenner et al. 1979) Brenner et al. 1989

strain designation: R67, Thacker 570-CO-H, 570-CO-H SG 12, 570-CO-H

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.999
121907negativerod-shapedno

colony morphology

  • @ref: 17945
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17945BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
42058MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121907CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
17945positivegrowth37mesophilic
42058positivegrowth37mesophilic
56534positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17945microaerophile
56534microaerophile
121907obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.967

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12190717632nitrate-reduction
12190716301nitrite-reduction

metabolite production

  • @ref: 121907
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
17945catalase+1.11.1.6
17945cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121907oxidase+
121907catalase+1.11.1.6
121907urease-3.5.1.5

fatty acid profile

  • @reffatty acidpercentageECL
    56534C16:03.316
    56534C17:00.817
    56534C18:0118
    56534C20:00.620
    56534C14:0 ISO6.713.618
    56534C15:0 ANTEISO9.914.711
    56534C15:0 ISO0.414.621
    56534C15:0 ISO 3OH216.135
    56534C15:1 ω6c2.814.856
    56534C16:0 iso30.615.626
    56534C16:1 ISO H6.115.461
    56534C16:1 ω7c13.515.819
    56534C16:1 ω9c0.715.774
    56534C17:0 anteiso4.316.722
    56534C17:0 CYCLO4.816.888
    56534C17:1 ω9c ANTEISO0.616.525
    56534Unidentified1.913.922
    56534Unidentified2.315.018
    56534Unidentified6.515.934
    56534Unidentified0.516.297
    56534Unidentified0.617.2
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG
  • @reffatty acidpercentageECL
    56534C14:00.214
    56534C15:00.515
    56534C16:03.316
    56534C17:00.817
    56534C18:01.118
    56534C19:00.319
    56534C20:00.720
    56534C14:0 ISO7.913.618
    56534C14:0 ISO 3OH1.315.117
    56534C14:1 ω5c0.813.899
    56534C15:0 ANTEISO11.814.711
    56534C15:0 ISO0.414.621
    56534C15:1 ω6c3.614.856
    56534C16:0 iso31.715.626
    56534C16:1 ISO H8.815.461
    56534C16:1 ω5c0.215.908
    56534C16:1 ω7c15.715.819
    56534C17:0 anteiso4.116.722
    56534C17:0 CYCLO4.716.888
    56534C17:0 iso0.216.629
    56534C17:1 ω9c ANTEISO0.916.525
    56534Unidentified0.410.53
    56534Unidentified0.216.309
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121907-+++-++---++--------

Isolation, sampling and environmental information

isolation

@refsample type
17945human lung tissue
50200Human lung tissue
56534Human lung tissue
121907Human, Lung tissue

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Lung
#Infection#Patient

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
179452Risk group (German classification)
1219072Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella pneumophila subsp. pneumophila ATCC 43290GCA_000239175completencbi933093
66792Legionella pneumophila subsp. pneumophila ATCC 43290GCA_000586115scaffoldncbi91891
66792Legionella pneumophila subsp. pneumophila ATCC 43290GCA_001997245contigncbi933093
66792Legionella pneumophila subsp. pneumophila ATCC 43290933093.3completepatric933093
66792Legionella pneumophila subsp. pneumophila ATCC 43290 strain ATCC 43290933093.8wgspatric933093
66792Legionella pneumophila subsp. pneumophila strain ATCC 4329091891.26wgspatric91891
66792Legionella pneumophila pneumonophila 570-CO-H, ATCC 432902511231146completeimg933093
66792Legionella pneumophila pneumophila ATCC 432902660238673draftimg91891

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes80.478no
flagellatedno67.991no
gram-positiveno98.561no
anaerobicno98.606yes
aerobicno91.811yes
halophileno88.376no
spore-formingno94.538no
glucose-utilyes69.419no
thermophileno98.044no
glucose-fermentno84.795no

External links

@ref: 17945

culture collection no.: DSM 25224, ATCC 43290, CCUG 30661, CIP 103866, CCUG 44896

straininfo link

  • @ref: 76193
  • straininfo: 46833

Reference

@idauthorscataloguedoi/urltitle
17945Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25224)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25224
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
42058Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15710
50200Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30661)https://www.ccug.se/strain?id=30661
56534Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44896)https://www.ccug.se/strain?id=44896
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76193Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46833.1StrainInfo: A central database for resolving microbial strain identifiers
121907Curators of the CIPCollection of Institut Pasteur (CIP 103866)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103866