Strain identifier

BacDive ID: 6717

Type strain: No

Species: Legionella pneumophila subsp. pneumophila

Strain Designation: R42, 797-PA-H SG11, 797-PA-H

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.L. Thacker: strain 797-PA-H <- E.J. Wing

NCBI tax ID(s): 91891 (subspecies)

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General

@ref: 17835

BacDive-ID: 6717

DSM-Number: 25063

keywords: genome sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella pneumophila subsp. pneumophila R42 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human endotracheal tube.

NCBI tax id

  • NCBI tax id: 91891
  • Matching level: subspecies

strain history

@refhistory
17835<- H. E. Müller; 797-PA-H SG11 <- ATCC <- W. L. Thacker <- E. J. Wing
121906CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- W.L. Thacker: strain 797-PA-H <- E.J. Wing

doi: 10.13145/bacdive6717.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella pneumophila subsp. pneumophila
  • full scientific name: Legionella pneumophila subsp. pneumophila (Brenner et al. 1979) Brenner et al. 1989

@ref: 17835

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella pneumophila subsp. pneumophila

full scientific name: Legionella pneumophila subsp. pneumophila (Brenner et al. 1979) Brenner et al. 1989

strain designation: R42, 797-PA-H SG11, 797-PA-H

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.998
121906negativerod-shapedno

colony morphology

  • @ref: 17835
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17835BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
37037MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121906CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
17835positivegrowth37mesophilic
37037positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 17835
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.981

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12190617632nitrate-reduction
12190616301nitrite-reduction

metabolite production

  • @ref: 121906
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
17835catalase+1.11.1.6
17835cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
121906oxidase-
121906catalase+1.11.1.6
121906urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121906-+---++---++--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
17835human endotracheal tube
50199Human endotracheal tube
121906Human, Endotracheal tubeUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Infection#Medical device
#Host#Human
#Host Body-Site#Oral cavity and airways#Trachea

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
178352Risk group (German classification)
1219062Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella pneumophila ATCC 43130GCA_001582215scaffoldncbi446
66792Legionella pneumophila subsp. pneumophila ATCC 43130GCA_000586155scaffoldncbi91891
66792Legionella pneumophila strain ATCC 43130446.147wgspatric446
66792Legionella pneumophila subsp. pneumophila strain ATCC 4313091891.28wgspatric91891
66792Legionella pneumophila pneumophila ATCC 431302667527774draftimg91891

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno76.157no
gram-positiveno98.326no
anaerobicno98.45yes
aerobicno91.056no
halophileno87.519no
spore-formingno94.131no
glucose-utilyes65.536no
motileyes77.578no
thermophileno98.578yes
glucose-fermentno86.511no

External links

@ref: 17835

culture collection no.: DSM 25063, ATCC 43130, CCUG 30660, CIP 103865

straininfo link

  • @ref: 76188
  • straininfo: 41667

Reference

@idauthorscataloguedoi/urltitle
17835Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25063)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25063
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37037Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15709
50199Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30660)https://www.ccug.se/strain?id=30660
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76188Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID41667.1StrainInfo: A central database for resolving microbial strain identifiers
121906Curators of the CIPCollection of Institut Pasteur (CIP 103865)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103865