Strain identifier

BacDive ID: 6715

Type strain: No

Species: Legionella pneumophila subsp. pneumophila

Strain Designation: R30, LE1 SG10, Leiden 1

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- H.W. Wilkinson, CDC: strain Leiden 1 <- P.L. Meenhorst

NCBI tax ID(s): 933091 (strain), 91891 (subspecies)

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General

@ref: 17833

BacDive-ID: 6715

DSM-Number: 25061

keywords: genome sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella pneumophila subsp. pneumophila R30 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from respiratory tract secretions.

NCBI tax id

NCBI tax idMatching level
933091strain
91891subspecies

strain history

@refhistory
17833<- H. E. Müller; LE1 SG10 <- ATCC; ATCC 43283 <- H. W. Wilkinson; <- P. L. Meenhorst;
121905CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- H.W. Wilkinson, CDC: strain Leiden 1 <- P.L. Meenhorst

doi: 10.13145/bacdive6715.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella pneumophila subsp. pneumophila
  • full scientific name: Legionella pneumophila subsp. pneumophila (Brenner et al. 1979) Brenner et al. 1989

@ref: 17833

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella pneumophila subsp. pneumophila

full scientific name: Legionella pneumophila subsp. pneumophila (Brenner et al. 1979) Brenner et al. 1989

strain designation: R30, LE1 SG10, Leiden 1

type strain: no

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.999
121905negativerod-shapedno

colony morphology

  • @ref: 17833
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17833BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
35151MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121905CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
17833positivegrowth37mesophilic
35151positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17833microaerophile
121905facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.987

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12190517632nitrate-reduction
12190516301nitrite-reduction

metabolite production

  • @ref: 121905
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
17833catalase+1.11.1.6
17833cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121905oxidase+/-
121905catalase+1.11.1.6
121905urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121905-+++-+++--++--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
17833respiratory tract secretionsNetherlandsNLDEurope
121905Other, Respiratory tract

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Oral cavity and airways#Airways
#Host Body Product#Fluids

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
178332Risk group (German classification)
1219052Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella pneumophila subsp. pneumophila ATCC 43283GCA_000586135scaffoldncbi91891
66792Legionella pneumophila subsp. pneumophila ATCC 43283GCA_001582165scaffoldncbi933091
66792Legionella pneumophila subsp. pneumophila ATCC 43283933091.3wgspatric933091
66792Legionella pneumophila subsp. pneumophila strain ATCC 4328391891.27wgspatric91891

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno70.775no
gram-positiveno98.71no
anaerobicno98.38yes
halophileno86.779no
spore-formingno94.748no
glucose-utilyes66.053no
motileyes80.801no
aerobicno93.637no
thermophileno96.119no
glucose-fermentno86.023no

External links

@ref: 17833

culture collection no.: DSM 25061, ATCC 43283, CIP 103864, NCTC 12000

straininfo link

  • @ref: 76186
  • straininfo: 92981

Reference

@idauthorscataloguedoi/urltitle
17833Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25061)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25061
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35151Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15708
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76186Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92981.1StrainInfo: A central database for resolving microbial strain identifiers
121905Curators of the CIPCollection of Institut Pasteur (CIP 103864)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103864