Strain identifier

BacDive ID: 6681

Type strain: Yes

Species: Liquorilactobacillus cacaonum

Strain Designation: R-34119

Strain history: <- K. De Bruyne and L. De Vuyst <- N. Camu, Vrije Univ. Brussels, Belgium; R-34119

NCBI tax ID(s): 1423729 (strain), 483012 (species)

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General

@ref: 15487

BacDive-ID: 6681

DSM-Number: 21116

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Liquorilactobacillus cacaonum R-34119 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from cocoa bean heap fermentation.

NCBI tax id

NCBI tax idMatching level
1423729strain
483012species

strain history

  • @ref: 15487
  • history: <- K. De Bruyne and L. De Vuyst <- N. Camu, Vrije Univ. Brussels, Belgium; R-34119

doi: 10.13145/bacdive6681.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Liquorilactobacillus
  • species: Liquorilactobacillus cacaonum
  • full scientific name: Liquorilactobacillus cacaonum (De Bruyne et al. 2009) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus cacaonum

@ref: 15487

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Liquorilactobacillus

species: Liquorilactobacillus cacaonum

full scientific name: Liquorilactobacillus cacaonum (De Bruyne et al. 2009) Zheng et al. 2020

strain designation: R-34119

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
28904positive2.5 µm0.9 µmrod-shapedno
69480positive100

pigmentation

  • @ref: 28904
  • production: no

Culture and growth conditions

culture medium

  • @ref: 15487
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15487positivegrowth30mesophilic
28904positivegrowth10-37
28904positiveoptimum23.5psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
15487microaerophile
28904facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.999

observation

  • @ref: 28904
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2890417057cellobiose+carbon source
2890428757fructose+carbon source
2890417234glucose+carbon source
2890417306maltose+carbon source
2890437684mannose+carbon source
2890417814salicin+carbon source
289044853esculin+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
15487-----------+++--------+--++++---------------------

Isolation, sampling and environmental information

isolation

  • @ref: 15487
  • sample type: cocoa bean heap fermentation
  • country: Ghana
  • origin.country: GHA
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_2983.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1207;97_1430;98_2298;99_2983&stattab=map
  • Last taxonomy: Liquorilactobacillus cacaonum
  • 16S sequence: AM905389
  • Sequence Identity:
  • Total samples: 516
  • soil counts: 5
  • aquatic counts: 11
  • animal counts: 358
  • plant counts: 142

Safety information

risk assessment

  • @ref: 15487
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15487
  • description: Lactobacillus cacaonum partial 16S rRNA gene, type strain LMG 24285T
  • accession: AM905389
  • length: 1527
  • database: ena
  • NCBI tax ID: 483012

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Liquorilactobacillus cacaonum DSM 21116GCA_001436735scaffoldncbi1423729
66792Lactobacillus cacaonum DSM 211161423729.3wgspatric1423729
66792Liquorilactobacillus cacaonum DSM 211162700989124draftimg1423729

GC content

@refGC-content
1548734.5
2890444.9

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno70no
flagellatedno88.513no
gram-positiveyes93.007yes
anaerobicno89.832yes
aerobicno96.026yes
halophileyes68.229no
spore-formingno90.735no
glucose-utilyes91.158no
motileyes52.375no
thermophileno97.718yes
glucose-fermentyes85.647no

External links

@ref: 15487

culture collection no.: DSM 21116, LMG 24285

straininfo link

  • @ref: 76155
  • straininfo: 311015

literature

  • topic: Phylogeny
  • Pubmed-ID: 19126714
  • title: Lactobacillus fabifermentans sp. nov. and Lactobacillus cacaonum sp. nov., isolated from Ghanaian cocoa fermentations.
  • authors: De Bruyne K, Camu N, De Vuyst L, Vandamme P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.001172-0
  • year: 2009
  • mesh: Bacterial Proteins/chemistry/genetics, Bacterial Typing Techniques, Cacao/*metabolism/*microbiology, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fermentation, Genes, rRNA, Ghana, Lactobacillus/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
15487Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21116)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21116
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
28904Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2534328776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
76155Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID311015.1StrainInfo: A central database for resolving microbial strain identifiers