Strain identifier

BacDive ID: 6670

Type strain: Yes

Species: Lacticaseibacillus camelliae

Strain Designation: MCH3-1

Strain history: CIP <- 2008, JCM <- 2006, S. Tanasupawat, Chulalongkorn Univ. Bangkok, Thailand: strain 13995

NCBI tax ID(s): 381742 (species)

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General

@ref: 16468

BacDive-ID: 6670

DSM-Number: 22697

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, Gram-positive

description: Lacticaseibacillus camelliae MCH3-1 is a microaerophile, Gram-positive bacterium that was isolated from fermented tea leaves .

NCBI tax id

  • NCBI tax id: 381742
  • Matching level: species

strain history

@refhistory
16468<- CIP <- S. Tanasupawat, Dept. Microbiology, Chulalongkorn Univ., Thailand; MCH3-1
67770S. Tanasupawat; Chulalongkorn Univ., Thailand; MCH3-1.
117111CIP <- 2008, JCM <- 2006, S. Tanasupawat, Chulalongkorn Univ. Bangkok, Thailand: strain 13995

doi: 10.13145/bacdive6670.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lacticaseibacillus
  • species: Lacticaseibacillus camelliae
  • full scientific name: Lacticaseibacillus camelliae (Tanasupawat et al. 2007) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus camelliae

@ref: 16468

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lacticaseibacillus

species: Lacticaseibacillus camelliae

full scientific name: Lacticaseibacillus camelliae (Tanasupawat et al. 2007) Zheng et al. 2020

strain designation: MCH3-1

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
117111positiverod-shapedno
69480no90.5
69480positive93.172

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16468MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
37868MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
117111CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
16468positivegrowth37
37868positivegrowth37
67770positivegrowth30
117111positivegrowth25-37
117111nogrowth15
117111nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16468microaerophile
117111facultative anaerobe

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 100

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose-builds acid from16988
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
117111nitrate-reduction17632
117111nitrite-reduction16301
117111nitrate+respiration17632

metabolite tests

  • @ref: 117111
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase+3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
117111oxidase-
117111alcohol dehydrogenase-1.1.1.1
117111catalase-1.11.1.6
117111lysine decarboxylase-4.1.1.18
117111ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117111-+---++---++---+++-+

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
16468------+---++++--------+++++++----------+----------
117111---+/--+---++++----+/----++/-+/-++++/--------+/-+/--+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
16468fermented tea leaves (miang)Chiang Mai, Tapair marketThailandTHAAsia
67770Fermented tea leaves (miang) in Thailand
117111Plant, Fermented tea leavesBangkokThailandTHAAsia2007

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production
#Host Body-Site#Plant#Leaf (Phyllosphere)

taxonmaps

  • @ref: 69479
  • File name: preview.99_5178.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_2517;97_3073;98_3859;99_5178&stattab=map
  • Last taxonomy: Lacticaseibacillus
  • 16S sequence: LC589224
  • Sequence Identity:
  • Total samples: 872
  • soil counts: 40
  • aquatic counts: 83
  • animal counts: 620
  • plant counts: 129

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
164681Risk group (German classification)
1171111Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
16468Lactobacillus camelliae gene for 16S rRNA, complete sequenceAB2578641530nuccore381742
67770Lacticaseibacillus camelliae JCM 13995 gene for 16S rRNA, partial sequenceLC5892241514nuccore381742

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus camelliae DSM 22697 = JCM 139951423730.4wgspatric1423730
66792Lactobacillus camelliae DSM 22697 = JCM 139951423730.3wgspatric1423730
66792Lacticaseibacillus camelliae JCM 139952728369674draftimg1423730
66792Lacticaseibacillus camelliae DSM 226972657244955draftimg1423730
67770Lacticaseibacillus camelliae DSM 22697 = JCM 13995GCA_001436615scaffoldncbi1423730
67770Lacticaseibacillus camelliae DSM 22697 = JCM 13995GCA_001311195contigncbi1423730

GC content

@refGC-contentmethod
1646851.9high performance liquid chromatography (HPLC)
6777055.4genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes93.172no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no80.404yes
69480spore-formingspore-formingAbility to form endo- or exosporesno87.89no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no93.526no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno90.5no

External links

@ref: 16468

culture collection no.: DSM 22697, BCC 21233, CIP 109883, JCM 13995, NRIC 0672, PCU 273

straininfo link

  • @ref: 76144
  • straininfo: 309462

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17429157Identification of lactic acid bacteria from fermented tea leaves (miang) in Thailand and proposals of Lactobacillus thailandensis sp. nov., Lactobacillus camelliae sp. nov., and Pediococcus siamensis sp. nov.Tanasupawat S, Pakdeeto A, Thawai C, Yukphan P, Okada SJ Gen Appl Microbiol10.2323/jgam.53.72007Base Composition, *Fermentation, Lactobacillus/classification/genetics/*isolation & purification/metabolism, Plant Leaves/*microbiology, RNA, Ribosomal, 16S/genetics, Tea/*microbiologyEnzymology
Phylogeny21724957Lactobacillus nasuensis sp. nov., a lactic acid bacterium isolated from silage, and emended description of the genus Lactobacillus.Cai Y, Pang H, Kitahara M, Ohkuma MInt J Syst Evol Microbiol10.1099/ijs.0.031781-02011Anaerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Gases/metabolism, Glucose/metabolism, Lactic Acid/*metabolism, Lactobacillus/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Silage/*microbiologyMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16468Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22697)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22697
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37868Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7676
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76144Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID309462.1StrainInfo: A central database for resolving microbial strain identifiers
117111Curators of the CIPCollection of Institut Pasteur (CIP 109883)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109883