Strain identifier

BacDive ID: 6662

Type strain: Yes

Species: Furfurilactobacillus siliginis

Strain Designation: M1-212, MI-212

Strain history: CIP <- 2006, NBRC <- Z. Aslam, Korea Adv. Inst. Sci. Technol., Daejeon, Korea: strain MI-212

NCBI tax ID(s): 348151 (species)

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General

@ref: 16467

BacDive-ID: 6662

DSM-Number: 22696

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, rod-shaped

description: Furfurilactobacillus siliginis M1-212 is an anaerobe, rod-shaped bacterium that was isolated from wheat sourdough.

NCBI tax id

  • NCBI tax id: 348151
  • Matching level: species

strain history

@refhistory
16467<- CIP <- NBRC <- Z. Aslam, KAIST; M1-212
67770KCTC 3985 <-- Z. Aslam M1-212.
67771<- Z Aslam, KAIST
122605CIP <- 2006, NBRC <- Z. Aslam, Korea Adv. Inst. Sci. Technol., Daejeon, Korea: strain MI-212

doi: 10.13145/bacdive6662.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Furfurilactobacillus
  • species: Furfurilactobacillus siliginis
  • full scientific name: Furfurilactobacillus siliginis (Aslam et al. 2006) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus siliginis

@ref: 16467

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Furfurilactobacillus

species: Furfurilactobacillus siliginis

full scientific name: Furfurilactobacillus siliginis (Aslam et al. 2006) Zheng et al. 2020

strain designation: M1-212, MI-212

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stainconfidence
67771rod-shapedno
67771positive
122605rod-shapednopositive
69480no92
69480positive93.251

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16467LACTOBACILLUS MEDIUM III (DSMZ Medium 638)yeshttps://mediadive.dsmz.de/medium/638Name: LACTOBACILLUS MEDIUM III (DSMZ Medium 638) Composition: Tryptone 10.0 g/l Glucose 7.0 g/l Maltose 7.0 g/l Fructose 7.0 g/l Na-acetate x 3 H2O 5.0 g/l Yeast extract 5.0 g/l Meat extract 5.0 g/l K2HPO4 x 3 H2O 2.6 g/l (NH4)2 citrate 2.0 g/l Na-gluconate 2.0 g/l L-Cysteine HCl x H2O 0.5 g/l MgSO4 x 7 H2O 0.1 g/l MnSO4 x 4 H2O 0.05 g/l Tween 80 Distilled water
34027MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122605CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
16467positivegrowth37
34027positivegrowth30
67770positivegrowth30
67771positivegrowth30

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16467anaerobe
67771facultative anaerobe

spore formation

@refspore formationconfidence
67771no
69481no100

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122605nitrate-reduction17632
122605nitrite-reduction16301

metabolite production

  • @ref: 122605
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
122605oxidase-
122605catalase-1.11.1.6
122605urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122605--+--+++--++---+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
16467-----+-----+----------+-----+------------------+--
122605----+----+/-+-+/---------+-----+------------------+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
16467wheat sourdoughDaejeonRepublic of KoreaKORAsia
67770Wheat sourdoughRepublic of KoreaKORAsia
67771From fermented foodRepublic of KoreaKORAsia
122605Food, Wheat-flour sample taken from standard Korean wheat-flour No. 3, produced by DaeHan Flour Mills, Korea (http://www.dhflour.co.kr)Republic of KoreaKORAsia2005

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Food

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
164671Risk group (German classification)
1226051Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus siliginis gene for 16S rRNA, partial sequence, strain: NBRC 101315AB6814461500nuccore348151
16467Lactobacillus siliginis strain M1-212 16S ribosomal RNA gene, partial sequenceDQ1680271310nuccore348151
16467Lactobacillus siligionis gene for 16S ribosomal RNA, partial sequence, strain: NBRC 101315AB3708821483nuccore348151
67770Lactobacillus siliginis gene for 16S ribosomal RNA, partial sequence, strain: JCM 16155LC2581521510nuccore348151
124043Lactobacillus siliginis genes for 16S rRNA, 16S-23S rRNA intergenic spacer, 23S rRNA, partial and complete sequence, culture_collection: JCM:16155.LC171392597nuccore348151

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus siliginis strain DSM 22696348151.3wgspatric348151
66792Lactobacillus siliginis strain NBRC 101315348151.5wgspatric348151
66792Furfurilactobacillus siliginis DSM 226962667528109draftimg348151
67770Furfurilactobacillus siliginis NBRC 101315GCA_007989765contigncbi348151
67770Furfurilactobacillus siliginis DSM 22696GCA_001437435scaffoldncbi348151

GC content

@refGC-contentmethod
1646744.5
6777045.4high performance liquid chromatography (HPLC)
6777044.1genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes93.251no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no75.557no
69480spore-formingspore-formingAbility to form endo- or exosporesno79.699yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no91.949yes
69480flagellatedmotile2+Ability to perform flagellated movementno92no

External links

@ref: 16467

culture collection no.: DSM 22696, CIP 109367, KCTC 3985, NBRC 101315, JCM 16155, NCIMB 14361

straininfo link

  • @ref: 76136
  • straininfo: 292427

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16957123Lactobacillus siliginis sp. nov., isolated from wheat sourdough in South Korea.Aslam Z, Im WT, Ten LN, Lee MJ, Kim KH, Lee STInt J Syst Evol Microbiol10.1099/ijs.0.64321-02006Base Composition, DNA, Bacterial/*analysis, *Food Microbiology, Korea, Lactobacillus/classification/*isolation & purification, Molecular Sequence Data, RNA, Ribosomal, 16S/*analysis/geneticsBiotechnology
Phylogeny28895518Lactobacillus curtus sp. nov., isolated from beer in Finland.Asakawa Y, Takesue N, Asano S, Shimotsu S, Iijima K, Suzuki K, Motoyama Y, Aizawa MInt J Syst Evol Microbiol10.1099/ijsem.0.0022202017Bacterial Typing Techniques, Base Composition, Beer/*microbiology, DNA, Bacterial/genetics, Finland, *Food Microbiology, Genes, Bacterial, Lactobacillus/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNABiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16467Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22696)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22696
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34027Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7100
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76136Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID292427.1StrainInfo: A central database for resolving microbial strain identifiers
122605Curators of the CIPCollection of Institut Pasteur (CIP 109367)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109367
124043Dr. Isabel Schober, Dr. Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy