Strain identifier

BacDive ID: 6599

Type strain: No

Species: Paucilactobacillus vaccinostercus

Strain Designation: MC11

Strain history: CIP <- 2002, CCUG, Lactobacillus durianis <- 2001, C. Vereecke/D.Janssens, LMG, Gent, Belgium <- M. Vancanneyt, Gent, Belgium

NCBI tax ID(s): 176291 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 6112

BacDive-ID: 6599

DSM-Number: 15802

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Paucilactobacillus vaccinostercus MC11 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from tempoyak.

NCBI tax id

  • NCBI tax id: 176291
  • Matching level: species

strain history

@refhistory
6112<- D. Janssens, LMG <- M. Vancanneyt, Univ. Ghent, Belgium <- J. J. Leisner
67770LMG 19193 <-- M. Vancanneyt R-9984 <-- J. Leisner MC11.
123770CIP <- 2002, CCUG, Lactobacillus durianis <- 2001, C. Vereecke/D.Janssens, LMG, Gent, Belgium <- M. Vancanneyt, Gent, Belgium

doi: 10.13145/bacdive6599.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Paucilactobacillus
  • species: Paucilactobacillus vaccinostercus
  • full scientific name: Paucilactobacillus vaccinostercus (Kozaki and Okada 1983) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus durianis
    20215Lactobacillus vaccinostercus

@ref: 6112

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Paucilactobacillus

species: Paucilactobacillus vaccinostercus

full scientific name: Paucilactobacillus vaccinostercus (Kozaki and Okada 1983) Zheng et al. 2020

strain designation: MC11

type strain: no

Morphology

cell morphology

  • @ref: 123770
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6112MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
39897MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
123770CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
6112positivegrowth30mesophilic
39897positivegrowth30mesophilic
56763positivegrowth28-37mesophilic
67770positivegrowth28mesophilic
123770positivegrowth22-37
123770nogrowth10psychrophilic
123770nogrowth15psychrophilic
123770nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6112microaerophile
56763anaerobe
123770facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123770nitrate-reduction17632
123770nitrite-reduction16301
123770nitrate+respiration17632

antibiotic resistance

  • @ref: 123770
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite tests

  • @ref: 123770
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
123770oxidase-
123770alcohol dehydrogenase-1.1.1.1
123770catalase-1.11.1.6
123770lysine decarboxylase-4.1.1.18
123770ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123770-----+++--++-++++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123770--+/-+++--+/-+/-+----------------+------------------+++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
6112tempoyakMalaysiaMYSAsia
56763TempoyakMalaysiaMYSAsia
123770TempoyakMalaysiaMYSAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Vegetable (incl. Grains)
#Engineered#Food production#Food
#Engineered#Food production#Fermented

taxonmaps

  • @ref: 69479
  • File name: preview.99_1677.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_563;97_653;98_1325;99_1677&stattab=map
  • Last taxonomy: Paucilactobacillus vaccinostercus
  • 16S sequence: AJ315640
  • Sequence Identity:
  • Total samples: 5611
  • soil counts: 307
  • aquatic counts: 795
  • animal counts: 4111
  • plant counts: 398

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
61121Risk group (German classification)
1237701Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus durianis strain LMG 19193 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequenceEU161604798ena176291
6112Lactobacillus durianis 16S rRNA gene, strain LMG 19193AJ3156401530ena176291

GC content

@refGC-contentmethod
611243.2-43.3
6777043.2high performance liquid chromatography (HPLC)

External links

@ref: 6112

culture collection no.: DSM 15802, CCUG 45405, CIP 107501, LMG 19193, JCM 12184

straininfo link

  • @ref: 76074
  • straininfo: 13289

literature

  • topic: Phylogeny
  • Pubmed-ID: 12054259
  • title: Lactobacillus durianis sp. nov., isolated from an acid-fermented condiment (tempoyak) in Malaysia.
  • authors: Leisner JJ, Vancanneyt M, Lefebvre K, Vandemeulebroecke K, Hoste B, Vilalta NE, Rusul G, Swings J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-3-927
  • year: 2002
  • mesh: DNA, Ribosomal/analysis, Electrophoresis, Gel, Pulsed-Field, Fermentation, Fruit/*microbiology, Lactic Acid/*metabolism, Lactobacillus/*classification/genetics/metabolism, Malaysia, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Plasmids/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6112Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15802)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15802
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39897Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4943
56763Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45405)https://www.ccug.se/strain?id=45405
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76074Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13289.1StrainInfo: A central database for resolving microbial strain identifiers
123770Curators of the CIPCollection of Institut Pasteur (CIP 107501)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107501