Strain identifier

BacDive ID: 6573

Type strain: Yes

Species: Schleiferilactobacillus perolens

Strain Designation: L 532

Strain history: LMG 18936 <-- W. Back et al. L 532.

NCBI tax ID(s): 1423792 (strain), 100468 (species)

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General

@ref: 4798

BacDive-ID: 6573

DSM-Number: 12744

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Schleiferilactobacillus perolens L 532 is a mesophilic bacterium that was isolated from orange lemonade.

NCBI tax id

NCBI tax idMatching level
100468species
1423792strain

strain history

@refhistory
4798<- I. Bohak <- W. Back; L 532
67770LMG 18936 <-- W. Back et al. L 532.

doi: 10.13145/bacdive6573.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Schleiferilactobacillus
  • species: Schleiferilactobacillus perolens
  • full scientific name: Schleiferilactobacillus perolens (Back et al. 2000) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus perolens

@ref: 4798

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Schleiferilactobacillus

species: Schleiferilactobacillus perolens

full scientific name: Schleiferilactobacillus perolens (Back et al. 2000) Zheng et al. 2020

strain designation: L 532

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no96.042
69480100positive

Culture and growth conditions

culture medium

  • @ref: 4798
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
4798positivegrowth28mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.996

murein

  • @ref: 4798
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose+builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose+builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
4798----------++++--------++++++++----+----+-+--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4798orange lemonadeGermanyDEUEurope
67770Spoiled soft drink

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Beverage

taxonmaps

  • @ref: 69479
  • File name: preview.99_95280.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_3115;97_3816;98_5655;99_95280&stattab=map
  • Last taxonomy: Schleiferilactobacillus perolens
  • 16S sequence: LC383833
  • Sequence Identity:
  • Total samples: 139
  • soil counts: 2
  • aquatic counts: 11
  • animal counts: 112
  • plant counts: 14

Safety information

risk assessment

  • @ref: 4798
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
4798Lactobacillus perolens 16S rRNA gene, strain L532Y191671566ena100468
67770Lactobacillus perolens JCM 12534 gene for 16S ribosomal RNA, partial sequenceLC3838331510ena100468

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus perolens DSM 127441423792.3wgspatric1423792
66792Lactobacillus perolens DSM 127442700989135draftimg1423792
67770Schleiferilactobacillus perolens DSM 12744GCA_001435585contigncbi1423792

GC content

@refGC-contentmethod
479851thermal denaturation, midpoint method (Tm)
6777049.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno92.604no
gram-positiveyes95.596no
anaerobicno90.728no
halophileyes77.791no
spore-formingno91.345no
glucose-utilyes92.257no
thermophileno96.36no
aerobicno93.803no
flagellatedno97.228no
glucose-fermentyes89.106no

External links

@ref: 4798

culture collection no.: DSM 12744, LMG 18936, JCM 12534

straininfo link

  • @ref: 76047
  • straininfo: 13211

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10553287Lactobacillus perolens sp. nov., a soft drink spoilage bacterium.Back W, Bohak I, Ehrmann M, Ludwig W, Pot B, Kersters K, Schleifer KHSyst Appl Microbiol10.1016/S0723-2020(99)80042-31999Bacterial Proteins/analysis, Base Sequence, Beverages/*microbiology, *Citrus, Electrophoresis, Polyacrylamide Gel, *Food Microbiology, Lactobacillus/chemistry/*classification/genetics, Molecular Sequence Data, Oligonucleotide Probes, Phylogeny, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, Sequence Homology, Nucleic AcidEnzymology
Metabolism16261858Lactobacillus harbinensis sp. nov., consisted of strains isolated from traditional fermented vegetables 'Suan cai' in Harbin, Northeastern China and Lactobacillus perolens DSM 12745.Miyamoto M, Seto Y, Hao DH, Teshima T, Sun YB, Kabuki T, Yao LB, Nakajima HSyst Appl Microbiol10.1016/j.syapm.2005.04.0012005Acetates/metabolism, Arabinose/metabolism, Base Composition, China, DNA Probes, DNA, Bacterial/genetics, Ethanol/metabolism, Fermentation, *Food Microbiology, Gluconates/metabolism, Glucose/metabolism, Lactic Acid/biosynthesis, Lactobacillus/*classification/genetics/metabolism, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Vegetables/metabolism/*microbiologyPhylogeny
Phylogeny23002044Lactobacillus shenzhenensis sp. nov., isolated from a fermented dairy beverage.Zou Y, Liu F, Fang C, Wan D, Yang R, Su Q, Yang R, Zhao JInt J Syst Evol Microbiol10.1099/ijs.0.041111-02012Bacterial Typing Techniques, Base Composition, Beverages/*microbiology, China, DNA, Bacterial/genetics, Dairy Products/*microbiology, Fatty Acids/analysis, *Fermentation, Food Microbiology, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNABiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4798Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12744)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12744
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76047Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13211.1StrainInfo: A central database for resolving microbial strain identifiers