Strain identifier

BacDive ID: 6537

Type strain: Yes

Species: Secundilactobacillus malefermentans

Strain Designation: D2 Mfl

Strain history: CIP <- 1989, NCFB <- T.K. Walker

NCBI tax ID(s): 176292 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 2298

BacDive-ID: 6537

DSM-Number: 5705

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Secundilactobacillus malefermentans D2 Mfl is a mesophilic bacterium that was isolated from beer.

NCBI tax id

  • NCBI tax id: 176292
  • Matching level: species

strain history

@refhistory
2298<- NCDO <- M.E. Sharpe <- T.K. Walker, D2 Mfl
67770NBRC 15905 <-- IFO 15905 <-- NRIC 1779 <-- NCDO 1410 <-- E. M. Sharpe <-- T. K. Walker.
120755CIP <- 1989, NCFB <- T.K. Walker

doi: 10.13145/bacdive6537.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Secundilactobacillus
  • species: Secundilactobacillus malefermentans
  • full scientific name: Secundilactobacillus malefermentans (Farrow et al. 1989 ex Russell and Walker 1953) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus malefermentans

@ref: 2298

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Secundilactobacillus

species: Secundilactobacillus malefermentans

full scientific name: Secundilactobacillus malefermentans (Farrow et al. 1989) Zheng et al. 2020

strain designation: D2 Mfl

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2298MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
39685MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120755CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
2298positivegrowth28mesophilic
39685positivegrowth30mesophilic
50897positivegrowth22psychrophilic
67770positivegrowth30mesophilic

Physiology and metabolism

murein

  • @ref: 2298
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG
2298-----+-----+----------------+------------------+-

Isolation, sampling and environmental information

isolation

@refsample type
2298beer
50897Beer
67770Beer
120755Other, Beer

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Beverage
#Engineered#Food production#Fermented
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1643.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_936;97_1089;98_1299;99_1643&stattab=map
  • Last taxonomy: Secundilactobacillus malefermentans
  • 16S sequence: LC383829
  • Sequence Identity:
  • Total samples: 2270
  • soil counts: 331
  • aquatic counts: 275
  • animal counts: 1580
  • plant counts: 84

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
22981Risk group (German classification)
1207551Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus malefermentans 16S rRNA gene, strain CECT 5928TAJ5757431525ena176292
20218Lactobacillus malefermentans partial 16S rRNA gene, strain type strain:DSM 5705AM1137831556ena176292
20218Lactobacillus malefermentans gene for 16S rRNA, partial sequence, strain: JCM 12497AB289194648ena176292
20218Lactobacillus malefermentans strain LMG 11455 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequenceEU161596841ena176292
20218Lactobacillus malefermentans gene for 16S rRNA, partial sequence, strain: NBRC 15905AB6809941491ena176292
67770Lactobacillus malefermentans JCM 12497 gene for 16S ribosomal RNA, partial sequenceLC3838291504ena176292

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus malefermentans DSM 5705 = KCTC 35481122149.3wgspatric1122149
66792Lactobacillus malefermentans KCTC 35481046594.4wgspatric1122149
66792Lactobacillus malefermentans strain ATCC 49373176292.3wgspatric176292
66792Secundilactobacillus malefermentans KCTC 35482513237370draftimg1122149
66792Secundilactobacillus malefermentans DSM 57052667527909draftimg1122149
67770Secundilactobacillus malefermentans DSM 5705 = KCTC 3548GCA_001436395contigncbi1122149
67770Schleiferilactobacillus malefermentans DSM 5705 = KCTC 3548 KCTC 3548GCA_000260775contigncbi1122149
67770Secundilactobacillus malefermentans ATCC 49373GCA_004354735scaffoldncbi176292

GC content

@refGC-contentmethod
229842.0
6777042thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.387no
flagellatedno98.159no
gram-positiveyes95.813no
anaerobicno94.193no
halophileyes91.047no
spore-formingno95.126no
thermophileno98.134no
glucose-utilyes89.947no
aerobicno96.965no
glucose-fermentyes83.018no

External links

@ref: 2298

culture collection no.: CCUG 32206, LMG 11455, CIP 103367, DSM 5705, ATCC 49373, NCDO 1410, JCM 12497, CECT 5928, IFO 15905, KCTC 3548, NBRC 15905, NCIMB 701410, NRIC 1779

straininfo link

  • @ref: 76009
  • straininfo: 3323

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics21914865Draft genome sequence of Lactobacillus malefermentans KCTC 3548.Kim DW, Choi SH, Kang A, Nam SH, Kim DS, Kim RN, Kim A, Park HSJ Bacteriol10.1128/JB.05710-112011Base Composition/genetics, Genome, Bacterial/*genetics, Lactobacillus/*genetics, Molecular Sequence Data
Phylogeny23378109Lactobacillus oryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica).Tohno M, Kitahara M, Irisawa T, Inoue H, Uegaki R, Ohkuma M, Tajima KInt J Syst Evol Microbiol10.1099/ijs.0.048918-02013Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, *Fermentation, Food Microbiology, Japan, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Oryza/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNABiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2298Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5705)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5705
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39685Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15157
50897Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32206)https://www.ccug.se/strain?id=32206
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76009Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3323.1StrainInfo: A central database for resolving microbial strain identifiers
120755Curators of the CIPCollection of Institut Pasteur (CIP 103367)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103367