Strain identifier

BacDive ID: 6522

Type strain: Yes

Species: Lacticaseibacillus sharpeae

Strain Designation: 71

Strain history: CIP <- 1985, DSMZ <- N. Weiss: strain 71

NCBI tax ID(s): 1626 (species)

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General

@ref: 8854

BacDive-ID: 6522

DSM-Number: 20505

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive

description: Lacticaseibacillus sharpeae 71 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from municipal sewage.

NCBI tax id

  • NCBI tax id: 1626
  • Matching level: species

strain history

@refhistory
8854<- N. Weiss, 71
67770DSM 20505 <-- N. Weiss 71.
67771<- CCUG <- JCM <- DSM
122583CIP <- 1985, DSMZ <- N. Weiss: strain 71

doi: 10.13145/bacdive6522.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lacticaseibacillus
  • species: Lacticaseibacillus sharpeae
  • full scientific name: Lacticaseibacillus sharpeae (Weiss et al. 1982) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus sharpeae

@ref: 8854

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lacticaseibacillus

species: Lacticaseibacillus sharpeae

full scientific name: Lacticaseibacillus sharpeae (Weiss et al. 1982) Zheng et al. 2020

strain designation: 71

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
67771positive
69480no96.241
69480positive100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8854MRS MEDIUM WITH CYSTEINE (DSMZ Medium 232)yeshttps://mediadive.dsmz.de/medium/232Name: MRS MEDIUM WITH CYSTEINE (DSMZ Medium 232) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)2 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38090MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinusyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
122583CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperaturerange
8854positivegrowth30mesophilic
38090positivegrowth30mesophilic
54805positivegrowth30mesophilic
67770positivegrowth30mesophilic
67771positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8854anaerobe
8854microaerophile
54805anaerobe
67771anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.997

murein

  • @ref: 8854
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122583-------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
8854municipal sewage
54805Sewage,municipalMünchenGermanyDEUEurope
67770Municipal sewage
67771From municipal sewage
122583Environment, Municipal sewageMünchenGermanyDEUEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_3917.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1999;97_2406;98_2975;99_3917&stattab=map
  • Last taxonomy: Lacticaseibacillus sharpeae subclade
  • 16S sequence: LC065040
  • Sequence Identity:
  • Total samples: 2428
  • soil counts: 219
  • aquatic counts: 168
  • animal counts: 1937
  • plant counts: 104

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
88541Risk group (German classification)
1225831Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus sharpeae 16S-23S rRNA intergenic spacer regionAF074861221ena1291052
20218Lactobacillus sharpeae strain DSM 20505 16S ribosomal RNA gene, partial sequenceM588311522ena1626
20218Lactobacillus sharpeae gene for 16S rRNA, partial sequence, strain: JCM 1186AB289301652ena1291052
67770Lactobacillus sharpeae gene for 16S ribosomal RNA, partial sequence, strain: JCM 1186LC0650401473ena1291052

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus sharpeae JCM 1186 = DSM 205051291052.5wgspatric1291052
66792Lactobacillus sharpeae JCM 1186 = DSM 205051291052.4wgspatric1291052
66792Lacticaseibacillus sharpeae JCM 1186, DSM 205052681813031draftimg1291052
66792Lacticaseibacillus sharpeae DSM 205052667527799draftimg1291052
67770Lacticaseibacillus sharpeae JCM 1186 = DSM 20505GCA_001436225scaffoldncbi1291052
67770Lacticaseibacillus sharpeae JCM 1186 = DSM 20505GCA_001311055contigncbi1291052

GC content

@refGC-contentmethod
885452.7
6777053.4genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.103no
gram-positiveyes95.073yes
anaerobicno89.018no
aerobicno97.422no
halophileyes88.122no
spore-formingno94.773no
glucose-utilyes88.827no
flagellatedno97.392no
thermophileno99.759yes
glucose-fermentyes87.055no

External links

@ref: 8854

culture collection no.: DSM 20505, ATCC 49974, JCM 1186, NCDO 2590, NCIB 11720, CCUG 39466, NCIMB 11720, KCTC 3803, BCRC 11662, CIP 101266, KCTC 5051, LMG 9214, NCFB 2590, NRIC 1641, NRRL B-14855

straininfo link

  • @ref: 75994
  • straininfo: 3369

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8854Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20505)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20505
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38090Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12822
54805Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39466)https://www.ccug.se/strain?id=39466
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75994Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3369.1StrainInfo: A central database for resolving microbial strain identifiers
122583Curators of the CIPCollection of Institut Pasteur (CIP 101266)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20101266