Strain identifier

BacDive ID: 6509

Type strain: No

Species: Limosilactobacillus reuteri

Strain Designation: 65 A

Strain history: CIP <- 2008, DSMZ <- Raibaud: strain 65 A

NCBI tax ID(s): 1598 (species)

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General

@ref: 8487

BacDive-ID: 6509

DSM-Number: 20056

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Limosilactobacillus reuteri 65 A is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from rat faeces.

NCBI tax id

  • NCBI tax id: 1598
  • Matching level: species

strain history

@refhistory
8487<- Raibaud, 65 A
122173CIP <- 2008, DSMZ <- Raibaud: strain 65 A

doi: 10.13145/bacdive6509.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Limosilactobacillus
  • species: Limosilactobacillus reuteri
  • full scientific name: Limosilactobacillus reuteri (Kandler et al. 1982) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus reuteri

@ref: 8487

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Limosilactobacillus

species: Limosilactobacillus reuteri

full scientific name: Limosilactobacillus reuteri (Kandler et al. 1982) Zheng et al. 2020

strain designation: 65 A

type strain: no

Morphology

cell morphology

  • @ref: 122173
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8487MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
36837MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122173CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
8487positivegrowth30
36837positivegrowth37
122173positivegrowth25-45
122173nogrowth15

Physiology and metabolism

oxygen tolerance

  • @ref: 122173
  • oxygen tolerance: facultative anaerobe

murein

  • @ref: 8487
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12217317632nitrate-reduction
12217316301nitrite-reduction
12217317632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite tests

  • @ref: 122173
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
122173oxidase-
122173alcohol dehydrogenase+1.1.1.1
122173catalase-1.11.1.6
122173lysine decarboxylase-4.1.1.18
122173ornithine decarboxylase-4.1.1.17
68382alpha-galactosidase+3.2.1.22

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122173--++-+----++++-+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8487-----+/-----+/-+----------------++/-+/-+/----+/------------+/---
122173----+---++-----------------++++---+-----------+--

Isolation, sampling and environmental information

isolation

@refsample type
8487rat faeces
122173Animal, Rat, faeces

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
84871Risk group (German classification)
1221731Risk group (French classification)

Sequence information

GC content

  • @ref: 8487
  • GC-content: 39.8

External links

@ref: 8487

culture collection no.: DSM 20056, CIP 109825

straininfo link

  • @ref: 75981
  • straininfo: 49571

Reference

@idauthorscataloguedoi/urltitle
8487Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20056)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20056
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36837Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7611
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
75981Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49571.1StrainInfo: A central database for resolving microbial strain identifiers
122173Curators of the CIPCollection of Institut Pasteur (CIP 109825)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109825