Strain identifier

BacDive ID: 6506

Type strain: No

Species: Limosilactobacillus reuteri

Strain Designation: 15/7

Strain history: CIP <- 2008, DSMZ <- I.G. Abo-Elnaga: strain 15/7, Lactobacillus fermentum

NCBI tax ID(s): 1598 (species)

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General

@ref: 8428

BacDive-ID: 6506

DSM-Number: 20015

keywords: Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Limosilactobacillus reuteri 15/7 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from manure.

NCBI tax id

  • NCBI tax id: 1598
  • Matching level: species

strain history

@refhistory
8428<- I. G. Abo-Elnaga, 15/7 (Lactobacillus fermentum)
122171CIP <- 2008, DSMZ <- I.G. Abo-Elnaga: strain 15/7, Lactobacillus fermentum

doi: 10.13145/bacdive6506.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Limosilactobacillus
  • species: Limosilactobacillus reuteri
  • full scientific name: Limosilactobacillus reuteri (Kandler et al. 1982) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus reuteri

@ref: 8428

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Limosilactobacillus

species: Limosilactobacillus reuteri

full scientific name: Limosilactobacillus reuteri (Kandler et al. 1982) Zheng et al. 2020

strain designation: 15/7

type strain: no

Morphology

cell morphology

  • @ref: 122171
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8428MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
41024MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122171CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
8428positivegrowth37
41024positivegrowth37
122171positivegrowth30-45
122171nogrowth15
122171nogrowth22

Physiology and metabolism

oxygen tolerance

  • @ref: 122171
  • oxygen tolerance: facultative anaerobe

murein

  • @ref: 8428
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12217117632nitrate-reduction
12217116301nitrite-reduction
12217117632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin+builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite tests

  • @ref: 122171
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
122171oxidase-
122171alcohol dehydrogenase+1.1.1.1
122171catalase-1.11.1.6
122171lysine decarboxylase-4.1.1.18
122171ornithine decarboxylase-4.1.1.17
68382alpha-galactosidase-3.2.1.22

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122171-----+----++-+------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122171----+----+++------------+--+++/-+---+/------------+--

Isolation, sampling and environmental information

isolation

@refsample type
8428manure
122171Manure

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Gastrointestinal tract
  • Cat3: #Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
84281Risk group (German classification)
1221711Risk group (French classification)

Sequence information

GC content

  • @ref: 8428
  • GC-content: 40.8

External links

@ref: 8428

culture collection no.: DSM 20015, CIP 109822

Reference

@idauthorscataloguedoi/urltitle
8428Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20015)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20015
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41024Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7608
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
122171Curators of the CIPCollection of Institut Pasteur (CIP 109822)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109822