Strain identifier

BacDive ID: 6468

Type strain: Yes

Species: Latilactobacillus graminis

Strain Designation: G90(1), G90

Strain history: CIP <- 1997, DSM <- R. Beck: strain G90

NCBI tax ID(s): 1423752 (strain), 60519 (species)

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General

@ref: 9029

BacDive-ID: 6468

DSM-Number: 20719

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Latilactobacillus graminis G90 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from grass silage.

NCBI tax id

NCBI tax idMatching level
60519species
1423752strain

strain history

@refhistory
9029<- R. Beck, G90(1)
67770NRIC 1775 <-- DSM 20719 <-- R. Beck G90(1).
119723CIP <- 1997, DSM <- R. Beck: strain G90

doi: 10.13145/bacdive6468.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Latilactobacillus
  • species: Latilactobacillus graminis
  • full scientific name: Latilactobacillus graminis (Beck et al. 1989) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus graminis

@ref: 9029

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Latilactobacillus

species: Latilactobacillus graminis

full scientific name: Latilactobacillus graminis (Beck et al. 1989) Zheng et al. 2020

strain designation: G90(1), G90

type strain: yes

Morphology

cell morphology

  • @ref: 119723
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9029MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
41545MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119723CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
9029positivegrowth30mesophilic
41545positivegrowth37mesophilic
50921positivegrowth37mesophilic
50921positivegrowth30-37mesophilic
67770positivegrowth30mesophilic
119723positivegrowth22-37
119723nogrowth10psychrophilic
119723nogrowth15psychrophilic
119723nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
50921anaerobe
50921microaerophile
119723facultative anaerobe

murein

  • @ref: 9029
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371maltose-builds acid from17306
68371cellobiose+builds acid from17057
68371esculin+builds acid from4853
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119723nitrate-reduction17632
119723nitrite-reduction16301
119723nitrate+respiration17632

metabolite tests

  • @ref: 119723
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
119723oxidase-
119723alcohol dehydrogenase-1.1.1.1
119723catalase-1.11.1.6
119723lysine decarboxylase-4.1.1.18
119723ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119723-----+----++--------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
9029------+---++++--------++++++-+--+------+-------+--
119723-----+/----+/-++/-+--------++/-+/-++/-+----+/-------+----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119723---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
9029grass silage
50921Grass silage
67770Grass silage
119723Grass silage

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Engineered#Biodegradation#Composting

taxonmaps

  • @ref: 69479
  • File name: preview.99_668.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_411;97_466;98_537;99_668&stattab=map
  • Last taxonomy: Latilactobacillus
  • 16S sequence: LC097076
  • Sequence Identity:
  • Total samples: 26696
  • soil counts: 1455
  • aquatic counts: 2674
  • animal counts: 21518
  • plant counts: 1049

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
90291Risk group (German classification)
1197231Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus graminis partial 16S rRNA gene, strain type strain:DSM 20719AM1137781548ena60519
20218Lactobacillus graminis 16S/23S ribosomal RNA small intergenic spacer region, complete sequenceU97130222ena60519
20218Lactobacillus graminis 16S/23S ribosomal RNA large intergenic spacer region, tRNA-Ile and tRNA-Ala genes, complete sequenceU97136437ena60519
20218Lactobacillus graminis gene for 16S rRNA, partial sequence, strain: JCM 9503AB289145651ena60519
20218Lactobacillus graminis partial 16S rRNA gene, strain LMG 9825TAJ6215511466ena60519
67770Lactobacillus graminis gene for 16S ribosomal RNA, partial sequence, strain: JCM 9503LC0970761511ena60519

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus graminis DSM 207191423752.3wgspatric1423752
66792Lactobacillus graminis DSM 207192671181128draftimg1423752
67770Latilactobacillus graminis DSM 20719GCA_001436415scaffoldncbi1423752

GC content

@refGC-contentmethod
902942.0
6777040.3genome sequence analysis
6777042thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno95.802no
gram-positiveyes94.107no
anaerobicno91.442no
halophileyes91.213no
spore-formingno94.599no
glucose-utilyes92.295no
thermophileno99.196yes
aerobicno98.47no
flagellatedno98.611no
glucose-fermentyes85.565no

External links

@ref: 9029

culture collection no.: DSM 20719, ATCC 51150, CIP 105164, NCIB 12808, CCUG 32238, JCM 9503, CECT 4017, KCTC 3542, LMG 9825, NCFB 2806, NCIMB 12808, NRIC 1775, NRRL B-14857

straininfo link

  • @ref: 75941
  • straininfo: 125622

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9029Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20719)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20719
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41545Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17153
50921Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32238)https://www.ccug.se/strain?id=32238
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75941Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID125622.1StrainInfo: A central database for resolving microbial strain identifiers
119723Curators of the CIPCollection of Institut Pasteur (CIP 105164)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105164