Strain identifier

BacDive ID: 6426

Type strain: No

Species: Lacticaseibacillus rhamnosus

Strain Designation: GK1

Strain history: <- NCFB <- Hillingdon Hospital, Uxbridge, UK, GK1

NCBI tax ID(s): 47715 (species)

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General

@ref: 3375

BacDive-ID: 6426

DSM-Number: 8746

keywords: Bacteria, mesophilic

description: Lacticaseibacillus rhamnosus GK1 is a mesophilic bacterium that was isolated from clinical source.

NCBI tax id

  • NCBI tax id: 47715
  • Matching level: species

strain history

  • @ref: 3375
  • history: <- NCFB <- Hillingdon Hospital, Uxbridge, UK, GK1

doi: 10.13145/bacdive6426.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lacticaseibacillus
  • species: Lacticaseibacillus rhamnosus
  • full scientific name: Lacticaseibacillus rhamnosus (Hansen 1968) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus casei subsp. rhamnosus
    20215Lactobacillus rhamnosus

@ref: 3375

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lacticaseibacillus

species: Lacticaseibacillus rhamnosus

full scientific name: Lacticaseibacillus rhamnosus (Hansen 1968) Zheng et al. 2020

strain designation: GK1

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3375COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
3375MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

  • @ref: 3375
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
3375---------+-++--+/---++-

Isolation, sampling and environmental information

isolation

  • @ref: 3375
  • sample type: clinical source

isolation source categories

  • Cat1: #Infection
  • Cat2: #Medical environment
  • Cat3: #Clinic

Safety information

risk assessment

  • @ref: 3375
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 3375

culture collection no.: DSM 8746, NCFB 2981

straininfo link

  • @ref: 75901
  • straininfo: 48538

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3375Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8746)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8746
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68368Automatically annotated from API 20E
75901Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48538.1StrainInfo: A central database for resolving microbial strain identifiers