Strain identifier
BacDive ID: 6426
Type strain:
Species: Lacticaseibacillus rhamnosus
Strain Designation: GK1
Strain history: <- NCFB <- Hillingdon Hospital, Uxbridge, UK, GK1
NCBI tax ID(s): 47715 (species)
version 9 (current version)
General
@ref: 3375
BacDive-ID: 6426
DSM-Number: 8746
keywords: Bacteria
description: Lacticaseibacillus rhamnosus GK1 is a bacterium that was isolated from clinical source.
NCBI tax id
- NCBI tax id: 47715
- Matching level: species
strain history
- @ref: 3375
- history: <- NCFB <- Hillingdon Hospital, Uxbridge, UK, GK1
doi: 10.13145/bacdive6426.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Lactobacillaceae
- genus: Lacticaseibacillus
- species: Lacticaseibacillus rhamnosus
- full scientific name: Lacticaseibacillus rhamnosus (Hansen 1968) Zheng et al. 2020
synonyms
@ref synonym 20215 Lactobacillus casei subsp. rhamnosus 20215 Lactobacillus rhamnosus
@ref: 3375
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Lactobacillaceae
genus: Lacticaseibacillus
species: Lacticaseibacillus rhamnosus
full scientific name: Lacticaseibacillus rhamnosus (Hansen 1968) Zheng et al. 2020
strain designation: GK1
type strain: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
3375 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
3375 | MRS MEDIUM (DSMZ Medium 11) | yes | https://mediadive.dsmz.de/medium/11 | Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water |
culture temp
- @ref: 3375
- growth: positive
- type: growth
- temperature: 37
Physiology and metabolism
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68368 | 30849 | L-arabinose | + | fermentation |
68368 | 27613 | amygdalin | + | fermentation |
68368 | 28053 | melibiose | - | fermentation |
68368 | 17992 | sucrose | - | fermentation |
68368 | 30911 | sorbitol | - | fermentation |
68368 | 17268 | myo-inositol | - | fermentation |
68368 | 16899 | D-mannitol | + | fermentation |
68368 | 17634 | D-glucose | + | fermentation |
68368 | 5291 | gelatin | - | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | - | hydrolysis |
68368 | 16947 | citrate | - | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | - | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | yes |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | + | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68368 | cytochrome oxidase | - | 1.9.3.1 |
68368 | gelatinase | - | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | - | 3.2.1.23 |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3375 | - | - | - | - | - | - | - | - | - | + | - | + | + | - | - | +/- | - | - | + | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 3375
- sample type: clinical source
isolation source categories
- Cat1: #Infection
- Cat2: #Medical environment
- Cat3: #Clinic
Safety information
risk assessment
- @ref: 3375
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 3375
culture collection no.: DSM 8746, NCFB 2981
straininfo link
- @ref: 75901
- straininfo: 48538
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3375 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8746) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-8746 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
68368 | Automatically annotated from API 20E | |||||
75901 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48538.1 | StrainInfo: A central database for resolving microbial strain identifiers |